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Protein

Methionine aminopeptidase

Gene

map

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62SubstrateUniRule annotation1
Metal bindingi82Divalent metal cation 1UniRule annotation1
Metal bindingi93Divalent metal cation 1UniRule annotation1
Metal bindingi93Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi153Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei161SubstrateUniRule annotation1
Metal bindingi187Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi280Divalent metal cation 1UniRule annotation1
Metal bindingi280Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidaseUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAPUniRule annotation
Short name:
MetAPUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:mapUniRule annotation
Ordered Locus Names:PYRAB14230
ORF Names:PAB1434
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489771 – 295Methionine aminopeptidaseAdd BLAST295

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272844.PAB1434.

Structurei

3D structure databases

ProteinModelPortaliQ9UYT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01001. Archaea.
COG0024. LUCA.
HOGENOMiHOG000226277.
KOiK01265.
OMAiERYKLHA.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_01975. MetAP_2_arc. 1 hit.
InterProiIPR028595. MetAP_archaeal.
IPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UYT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVDKLIEAG KIAKKVREEA VKLAKPGVSL LELAEKIEGR IIELGAKPAF
60 70 80 90 100
PVNLSLNEIA AHYTPYKGDE TTLKEGDYLK IDIGVHIDGY IADTAVTVRV
110 120 130 140 150
GMEEDDLMEA AREALESAIS VARAGVEIKE LGRAIEDEIR KRGFNPIVNL
160 170 180 190 200
SGHKIERYKL HAGISIPNIY RPHDNYKLRE GDVFAIEPFA TTGAGQVIEV
210 220 230 240 250
PPTLIYMYVR DAPVRMVQAR FLLAKIKREY KTLPFAYRWL QGEMPEGQLK
260 270 280 290
LALRTLEKSG ALYGYPVLRE IRNGLVTQFE HTIIVEKDSV IVTTE
Length:295
Mass (Da):33,027
Last modified:May 1, 2000 - v1
Checksum:i2670D3E7DE6BF7B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248287 Genomic DNA. Translation: CAB50328.1.
HE613800 Genomic DNA. Translation: CCE70868.1.
PIRiC75054.
RefSeqiWP_010868538.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB50328; CAB50328; PAB1434.
GeneIDi1496814.
KEGGipab:PAB1434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248287 Genomic DNA. Translation: CAB50328.1.
HE613800 Genomic DNA. Translation: CCE70868.1.
PIRiC75054.
RefSeqiWP_010868538.1. NC_000868.1.

3D structure databases

ProteinModelPortaliQ9UYT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB1434.

Protein family/group databases

MEROPSiM24.035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB50328; CAB50328; PAB1434.
GeneIDi1496814.
KEGGipab:PAB1434.

Phylogenomic databases

eggNOGiarCOG01001. Archaea.
COG0024. LUCA.
HOGENOMiHOG000226277.
KOiK01265.
OMAiERYKLHA.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_01975. MetAP_2_arc. 1 hit.
InterProiIPR028595. MetAP_archaeal.
IPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAP2_PYRAB
AccessioniPrimary (citable) accession number: Q9UYT4
Secondary accession number(s): G8ZHN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.