Q9UYT4 (AMPM_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Ordered Locus Names:	PYRAB14230
ORF Names:	PAB1434

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Methionine aminopeptidase		PRO_0000148977
Sites
	Metal binding	82	1	Cobalt 1 By similarity
	Metal binding	93	1	Cobalt 1 By similarity
	Metal binding	93	1	Cobalt 2 By similarity
	Metal binding	153	1	Cobalt 2 By similarity
	Metal binding	187	1	Cobalt 2 By similarity
	Metal binding	280	1	Cobalt 1 By similarity
	Metal binding	280	1	Cobalt 2 By similarity
	Binding site	62	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UYT4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2670D3E7DE6BF7B3	FASTA	295	33,027
	10 20 30 40 50 60 MDVDKLIEAG KIAKKVREEA VKLAKPGVSL LELAEKIEGR IIELGAKPAF PVNLSLNEIA 70 80 90 100 110 120 AHYTPYKGDE TTLKEGDYLK IDIGVHIDGY IADTAVTVRV GMEEDDLMEA AREALESAIS 130 140 150 160 170 180 VARAGVEIKE LGRAIEDEIR KRGFNPIVNL SGHKIERYKL HAGISIPNIY RPHDNYKLRE 190 200 210 220 230 240 GDVFAIEPFA TTGAGQVIEV PPTLIYMYVR DAPVRMVQAR FLLAKIKREY KTLPFAYRWL 250 260 270 280 290 QGEMPEGQLK LALRTLEKSG ALYGYPVLRE IRNGLVTQFE HTIIVEKDSV IVTTE

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248287 Genomic DNA. Translation: CAB50328.1.
PIR	C75054.
RefSeq	NP_127098.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UYT4.
SMR	Q9UYT4. Positions 1-295.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002986; EBPYRP00000002917; EBPYRG00000002986.
GeneID	1496814.
GenomeReviews	Gene locus PYRAB14230 in contig AL096836_GR.
KEGG	pab:PAB1434.
NMPDR	fig|272844.1.peg.1507.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022572.
HOGENOM	HBG318153.
OMA	IEPFATN.
PhylomeDB	Q9UYT4.
ProtClustDB	PRK08671.
Enzyme and pathway databases
BioCyc	PABY272844:PAB1434-MONOMER.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002468. Pept_M24A_MAP2. IPR018349. Pept_M24A_MAP2_BS. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 2 hits. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01265.
PANTHER	PTHR10804:SF9. Pept_M24A_MAP2. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00501. Met_pdase_II. 1 hit.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_PYRAB

Accession

Primary (citable) accession number: Q9UYT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program