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Q9UYS8 (TOP1_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:PYRAB14290
ORF Names:PAB1430
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length685 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 685685DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145180

Regions

Domain1 – 141141Toprim
Zinc finger611 – 63929C4-type 1 HAMAP-Rule MF_00952
Zinc finger658 – 68225C4-type 2; atypical HAMAP-Rule MF_00952
Region196 – 2016Interaction with DNA By similarity

Sites

Active site3171O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding71Magnesium 1; catalytic By similarity
Metal binding1071Magnesium 1; catalytic By similarity
Metal binding1071Magnesium 2 By similarity
Metal binding1091Magnesium 2 By similarity
Site521Interaction with DNA By similarity
Site1661Interaction with DNA By similarity
Site1701Interaction with DNA By similarity
Site3191Interaction with DNA By similarity
Site5071Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9UYS8 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 4888C43AB87B485D

FASTA68578,196
        10         20         30         40         50         60 
MILVIAEKPN VARKIAGALS ERRPIRKTIF GVPYYEVFRD GKKLIVASAV GHLYGLAPKQ 

        70         80         90        100        110        120 
DFFGYPIFDI EWVPVYIAEK NKDYAKDYIK ALSVLAKRVK EFIVACDYDT EGEVIGYTAL 

       130        140        150        160        170        180 
KYACGVDPSR AKRMKFSALT KRDLLNAWRN LEPTINFGMA NAGIARHVLD WYWGVNLSRA 

       190        200        210        220        230        240 
LTHAIKRASG KWVVLSTGRV QGPTLKFLVE REREIQSFVP KPYWVIKLVI EKNGQKLVAN 

       250        260        270        280        290        300 
YEKDKIWSEE EAKKIVTEVK KSKARVSNIE VKRQKRNPPV PFDLGTLQRE AYSAFGFSPK 

       310        320        330        340        350        360 
KTLDIAQSLY EKGFTSYPRT ESQKLPKNLN FRLIIQNISR MPQYRPYAHL LLGMPELKPV 

       370        380        390        400        410        420 
EGKKEDPAHP AIYPTGEIPG PGDLSKDEEK LYDMIVRRFL ALFMEPAVRE SVKVTILAGP 

       430        440        450        460        470        480 
HKFMLSGART VKQGWLSVYG KYIKFDEVTL PEFFIGERVR VLQVRREKKK TKPPARYSPA 

       490        500        510        520        530        540 
AVIKKMEDLG IGTKATRAQI LETLYQRGYI EGKKSIKVTP LGMKVIETLE KYVPEIISVE 

       550        560        570        580        590        600 
LTREFERKMD LIMQGKLTKE EVIEEAKVKL TKILEEFKKR ELEIGLELAR IVVGDVKDEI 

       610        620        630        640        650        660 
KEEKPIVVGK CPKCGGDLIV KYNKKTGKRF VGCSNWPKCD VTYPILQRGE IIPTNKTCCN 

       670        680 
GAPVVIIRDK GRDIEICLDI KCKKW 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248287 Genomic DNA. Translation: CAB50334.1.
HE613800 Genomic DNA. Translation: CCE70874.1.
PIRA75055.
RefSeqNP_127104.1. NC_000868.1.

3D structure databases

ProteinModelPortalQ9UYS8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB1430.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB50334; CAB50334; PAB1430.
GeneID1496820.
KEGGpab:PAB1430.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004019.
KOK03168.
OMAYIESDPP.
ProtClustDBPRK05776.

Family and domain databases

Gene3D1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005739. TopoI_arch.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 2 hits.
TIGRFAMsTIGR01057. topA_arch. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_PYRAB
AccessionPrimary (citable) accession number: Q9UYS8
Secondary accession number(s): G8ZHN7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: February 19, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families