Reviewed, UniProtKB/Swiss-Prot Q9UYS8 (TOP1_PYRAB)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	PYRAB14290
ORF Names:	PAB1430

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 685	685	DNA topoisomerase 1		PRO_0000145180
Regions
	Zinc finger	611 – 639	29	C4-type 1
	Zinc finger	658 – 682	25	C4-type 2; atypical
Sites
	Active site	317	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UYS8-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 4888C43AB87B485D	FASTA	685	78,196
	10 20 30 40 50 60 MILVIAEKPN VARKIAGALS ERRPIRKTIF GVPYYEVFRD GKKLIVASAV GHLYGLAPKQ 70 80 90 100 110 120 DFFGYPIFDI EWVPVYIAEK NKDYAKDYIK ALSVLAKRVK EFIVACDYDT EGEVIGYTAL 130 140 150 160 170 180 KYACGVDPSR AKRMKFSALT KRDLLNAWRN LEPTINFGMA NAGIARHVLD WYWGVNLSRA 190 200 210 220 230 240 LTHAIKRASG KWVVLSTGRV QGPTLKFLVE REREIQSFVP KPYWVIKLVI EKNGQKLVAN 250 260 270 280 290 300 YEKDKIWSEE EAKKIVTEVK KSKARVSNIE VKRQKRNPPV PFDLGTLQRE AYSAFGFSPK 310 320 330 340 350 360 KTLDIAQSLY EKGFTSYPRT ESQKLPKNLN FRLIIQNISR MPQYRPYAHL LLGMPELKPV 370 380 390 400 410 420 EGKKEDPAHP AIYPTGEIPG PGDLSKDEEK LYDMIVRRFL ALFMEPAVRE SVKVTILAGP 430 440 450 460 470 480 HKFMLSGART VKQGWLSVYG KYIKFDEVTL PEFFIGERVR VLQVRREKKK TKPPARYSPA 490 500 510 520 530 540 AVIKKMEDLG IGTKATRAQI LETLYQRGYI EGKKSIKVTP LGMKVIETLE KYVPEIISVE 550 560 570 580 590 600 LTREFERKMD LIMQGKLTKE EVIEEAKVKL TKILEEFKKR ELEIGLELAR IVVGDVKDEI 610 620 630 640 650 660 KEEKPIVVGK CPKCGGDLIV KYNKKTGKRF VGCSNWPKCD VTYPILQRGE IIPTNKTCCN 670 680 GAPVVIIRDK GRDIEICLDI KCKKW

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ248287 Genomic DNA. Translation: CAB50334.1.
PIR	A75055.
RefSeq	NP_127104.1.
3D structure databases
HSSP	HSSP built from PDB template 1CY1 based on UniProtKB P06612.
ModBase	Search...
Genome annotation databases
GeneID	1496820.
GenomeReviews	Gene locus PYRAB14290 in contig AL096836_GR.
KEGG	pab:PAB1430.
NMPDR	fig|272844.1.peg.1513.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9UYS8.
OMA	LIICEKP.
Enzyme and pathway databases
BioCyc	PABY272844:PAB1430-MON.
BRENDA	5.99.1.2. 262861.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005739. TopoI_arch. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01057. topA_arch. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_PYRAB

Accession

Primary (citable) accession number: Q9UYS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)