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Protein

GPN-loop GTPase PAB0955

Gene

PYRAB14380

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Small GTPase that may be involved in genome maintenance. Has weak intrinsic GTPase activity but displays no ATPase activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei67 – 671Stabilizes the phosphate intermediate; shared with dimeric partner1 Publication
Binding sitei224 – 2241GTP; via amide nitrogen1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156GTP1 Publication
Nucleotide bindingi165 – 1684GTP1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GPN-loop GTPase PAB09551 Publication (EC:3.6.5.-1 Publication)
Gene namesi
Ordered Locus Names:PYRAB14380Imported
ORF Names:PAB0955Imported
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248GPN-loop GTPase PAB0955PRO_0000431297Add
BLAST

Interactioni

Subunit structurei

Homodimer (PubMed:16510996). Interacts with DNA topoisomerase VI subunit B (top6B), DNA primase DnaG and RF-C (PubMed:17468740).2 Publications

Protein-protein interaction databases

MINTiMINT-4791347.
STRINGi272844.PAB0955.

Structurei

Secondary structure

248
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi1 – 66Combined sources
Helixi13 – 2412Combined sources
Turni25 – 273Combined sources
Beta strandi30 – 345Combined sources
Beta strandi47 – 493Combined sources
Helixi50 – 523Combined sources
Helixi56 – 605Combined sources
Turni61 – 633Combined sources
Helixi66 – 7813Combined sources
Helixi81 – 9414Combined sources
Beta strandi96 – 1016Combined sources
Helixi106 – 1116Combined sources
Helixi113 – 1208Combined sources
Beta strandi122 – 1243Combined sources
Beta strandi126 – 1316Combined sources
Helixi133 – 1353Combined sources
Helixi139 – 15618Combined sources
Beta strandi160 – 1645Combined sources
Helixi167 – 1693Combined sources
Helixi172 – 18312Combined sources
Helixi185 – 19410Combined sources
Helixi198 – 21316Combined sources
Turni224 – 2263Combined sources
Helixi230 – 24415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YR6X-ray2.15A1-248[»]
1YR7X-ray2.08A1-248[»]
1YR8X-ray2.40A1-248[»]
1YR9X-ray2.80A1-248[»]
1YRAX-ray2.30A/B1-248[»]
1YRBX-ray1.75A/B1-248[»]
2OXRX-ray2.40A1-248[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UYR9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi65 – 673Gly-Pro-Asn (GPN)-loop; involved in dimer interface1 Publication

Sequence similaritiesi

Belongs to the GPN-loop GTPase family.Curated

Phylogenomic databases

eggNOGiarCOG01225. Archaea.
COG1100. LUCA.
HOGENOMiHOG000110575.
KOiK06883.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR004130. Gpn.
IPR030228. Gpn3.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR21231. PTHR21231. 1 hit.
PTHR21231:SF7. PTHR21231:SF7. 1 hit.
PfamiPF03029. ATP_bind_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UYR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVVFVGTAG SGKTTLTGEF GRYLEDNYKV AYVNLDTGVK ELPYEPSIDV
60 70 80 90 100
REFVTVEEIM REGYGPNGAI VESYDRLMEK FNEYLNKILR LEKENDYVLI
110 120 130 140 150
DTPGQMETFL FHEFGVRLME NLPYPLVVYI SDPEILKKPN DYCFVRFFAL
160 170 180 190 200
LIDLRLGATT IPALNKVDLL SEEEKERHRK YFEDIDYLTA RLKLDPSMQG
210 220 230 240
LMAYKMCSMM TEVLPPVRVL YLSAKTREGF EDLETLAYEH YCTCGDLT
Length:248
Mass (Da):28,697
Last modified:November 26, 2014 - v2
Checksum:iF88FB5473E40B0D3
GO

Sequence cautioni

The sequence CAB50343.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248287 Genomic DNA. Translation: CAB50343.1. Different initiation.
HE613800 Genomic DNA. Translation: CCE70884.1.
PIRiB75056.
RefSeqiWP_048147021.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB50343; CAB50343; PAB0955.
GeneIDi1495713.
KEGGipab:PAB0955.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248287 Genomic DNA. Translation: CAB50343.1. Different initiation.
HE613800 Genomic DNA. Translation: CCE70884.1.
PIRiB75056.
RefSeqiWP_048147021.1. NC_000868.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YR6X-ray2.15A1-248[»]
1YR7X-ray2.08A1-248[»]
1YR8X-ray2.40A1-248[»]
1YR9X-ray2.80A1-248[»]
1YRAX-ray2.30A/B1-248[»]
1YRBX-ray1.75A/B1-248[»]
2OXRX-ray2.40A1-248[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4791347.
STRINGi272844.PAB0955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB50343; CAB50343; PAB0955.
GeneIDi1495713.
KEGGipab:PAB0955.

Phylogenomic databases

eggNOGiarCOG01225. Archaea.
COG1100. LUCA.
HOGENOMiHOG000110575.
KOiK06883.

Miscellaneous databases

EvolutionaryTraceiQ9UYR9.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR004130. Gpn.
IPR030228. Gpn3.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR21231. PTHR21231. 1 hit.
PTHR21231:SF7. PTHR21231:SF7. 1 hit.
PfamiPF03029. ATP_bind_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.
  3. "Expression, purification, crystallization and preliminary crystallographic analysis of the PAB0955 gene product."
    Gras S., Fernandez B., Chaumont V., Carpentier P., Armengaud J., Housset D.
    Acta Crystallogr. F 61:208-211(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 1-248 IN COMPLEX WITH GTP, CATALYTIC ACTIVITY, INTERACTION WITH TOP6B; DNAG AND RF-C.

Entry informationi

Entry nameiGPN_PYRAB
AccessioniPrimary (citable) accession number: Q9UYR9
Secondary accession number(s): G8ZIK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 26, 2014
Last sequence update: November 26, 2014
Last modified: January 20, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.