Q9UYR1 (GYAR_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxylate reductase
EC=1.1.1.26

Gene names

Name:	gyaR
Ordered Locus Names:	PYRAB14460
ORF Names:	PAB2374

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Glycolate + NAD+ = glyoxylate + NADH. HAMAP MF_00776
Subunit structure	Homodimer By similarity. HAMAP MF_00776
Subcellular location	Cytoplasm By similarity HAMAP MF_00776.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Glyoxylate reductase HAMAP MF_00776		PRO_0000075947
Regions
	Nucleotide binding	159 – 162	4	NADP By similarity
	Nucleotide binding	181 – 183	3	NADP By similarity
	Nucleotide binding	240 – 242	3	NADP By similarity
	Nucleotide binding	289 – 291	3	NADP By similarity
Sites
	Active site	242	1	By similarity
	Active site	271	1	By similarity
	Active site	289	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UYR1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 32CEA1CB7126A0B4	FASTA	335	38,270
	10 20 30 40 50 60 MSKPRVFITR EIPEVGIEML EKEFEVEVWE DEREIPREIL LEKVKDVDAL VTMLSERIDR 70 80 90 100 110 120 EVFERAPRLR IVANYAVGYD NIDVEEATKR GIYVTNTPGV LTDATADLAF ALLLATARHL 130 140 150 160 170 180 VKGDKFTRSG EWKKRGVAWH PKWFLGYDVY GKTIGIIGFG RIGQAIAKRA RGFDMRILYY 190 200 210 220 230 240 SRTRKPEVEK ELNAEFKPLD ELLRESDFVV LAVPLNKETY HMINEERLKM MKRTAILINV 250 260 270 280 290 300 ARGKVIDTKA LIKALKEGWI AGAGLDVYEE EPYYNEELFS LDNVVLTPHI GSATFGAREG 310 320 330 MAKLVAENLI AFKRGEVPPT LVNREVLKVR KPGFQ

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248287 Genomic DNA. Translation: CAB50351.1.
PIR	B75057.
RefSeq	NP_127121.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UYR1.
SMR	Q9UYR1. Positions 3-334.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002504; EBPYRP00000002435; EBPYRG00000002504.
GeneID	1495721.
GenomeReviews	Gene locus PYRAB14460 in contig AL096836_GR.
KEGG	pab:PAB2374.
NMPDR	fig|272844.1.peg.1531.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022341.
HOGENOM	HBG731446.
OMA	TYSVGYD.
PhylomeDB	Q9UYR1.
ProtClustDB	PRK13243.
Enzyme and pathway databases
BioCyc	PABY272844:PAB2374-MONOMER.
Family and domain databases
HAMAP	MF_00776. GyaR. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR023519. Glyoxylate_reductase_GyaR. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K00015.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYAR_PYRAB

Accession

Primary (citable) accession number: Q9UYR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program