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Protein

tRNA (cytidine(56)-2'-O)-methyltransferase

Gene

PYRAB15770

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytidine(56) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(56) in tRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • tRNA methyltransferase activity Source: UniProtKB

GO - Biological processi

  • tRNA nucleoside ribose methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.206. 5242.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(56)-2'-O)-methyltransferase (EC:2.1.1.206)
Alternative name(s):
tRNA ribose 2'-O-methyltransferase aTrm56
Gene namesi
Ordered Locus Names:PYRAB15770
ORF Names:PAB1040
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001469331 – 198tRNA (cytidine(56)-2'-O)-methyltransferaseAdd BLAST198

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272844.PAB1040.

Structurei

3D structure databases

ProteinModelPortaliQ9UYD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni110 – 114S-adenosyl-L-methionine bindingBy similarity5
Regioni128 – 135S-adenosyl-L-methionine bindingBy similarity8

Sequence similaritiesi

Belongs to the aTrm56 family.Curated

Phylogenomic databases

eggNOGiarCOG01857. Archaea.
COG1303. LUCA.
HOGENOMiHOG000015482.
KOiK07254.
OMAiLGHRPER.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00077. tRNA_methyltr_aTrm56. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR029026. tRNA_m1G_MTases_N.
IPR002845. tRNA_mtfrase_aTrm56.
[Graphical view]
PfamiPF01994. Trm56. 1 hit.
[Graphical view]
PIRSFiPIRSF016123. UCP016123. 1 hit.
ProDomiPD016584. DUF127. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF75217. SSF75217. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UYD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVVLRLGHR PERDKRVTTH VALTARAFGA DGIIIVSEEV DLKVKESVED
60 70 80 90 100
VVERWGGPFF VKFEKSWRKV MKEFDGVKVH LTMYGIHIDD IIDELREKLR
110 120 130 140 150
EGRDFMVIVG AEKVPREVYE LADYNVAIGN QPHSEVAALA VFLDRLLEGK
160 170 180 190
GLRKEFKGAK LKIIPQARGK MVVEVQKDAK QAEASGEGAS RKNGQLPS
Length:198
Mass (Da):22,262
Last modified:May 1, 2000 - v1
Checksum:iD126B48B3A0D5AAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50481.1.
HE613800 Genomic DNA. Translation: CCE71034.1.
PIRiC75005.
RefSeqiWP_010868694.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB50481; CAB50481; PAB1040.
GeneIDi1495863.
KEGGipab:PAB1040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50481.1.
HE613800 Genomic DNA. Translation: CCE71034.1.
PIRiC75005.
RefSeqiWP_010868694.1. NC_000868.1.

3D structure databases

ProteinModelPortaliQ9UYD1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB1040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB50481; CAB50481; PAB1040.
GeneIDi1495863.
KEGGipab:PAB1040.

Phylogenomic databases

eggNOGiarCOG01857. Archaea.
COG1303. LUCA.
HOGENOMiHOG000015482.
KOiK07254.
OMAiLGHRPER.

Enzyme and pathway databases

BRENDAi2.1.1.206. 5242.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_00077. tRNA_methyltr_aTrm56. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR029026. tRNA_m1G_MTases_N.
IPR002845. tRNA_mtfrase_aTrm56.
[Graphical view]
PfamiPF01994. Trm56. 1 hit.
[Graphical view]
PIRSFiPIRSF016123. UCP016123. 1 hit.
ProDomiPD016584. DUF127. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF75217. SSF75217. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRM56_PYRAB
AccessioniPrimary (citable) accession number: Q9UYD1
Secondary accession number(s): G8ZJ01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.