Reviewed, UniProtKB/Swiss-Prot Q9UYC6 (LONH_PYRAB)

Names and origin

Protein names

Recommended name:
    Putative protease La homolog
    EC=3.4.21.-
Cleaved into the following chain:
    1- Recommended name:
            Pab lon intein

Gene names

Ordered Locus Names:	PYRAB15820
ORF Names:	PAB1313

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	998 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	Intron homing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease Protease Serine protease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro transcription factor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Putative protease La homolog, 1st part Potential		PRO_0000026726
	Chain	221 – 553	333	Pab lon intein Potential		PRO_0000026727
	Chain	554 – 998	445	Putative protease La homolog, 2nd part Potential		PRO_0000026728
Regions
	Domain	370 – 496	127	DOD-type homing endonuclease
	Nucleotide binding	69 – 76	8	ATP Potential
Sites
	Active site	859	1	By similarity
	Active site	902	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UYC6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BDB15A8B00D135F9	FASTA	998	111,704
	10 20 30 40 50 60 MLKFYKMGIK RLSGEMTMGE ERMDLGIEFE TTEEIPVPER LIDQVIGQDH AVEVIKTAAK 70 80 90 100 110 120 QRRHVLLIGE PGTGKSMLGQ AMAELLPTED LEDILVFPNP EDENMPRIKT VPAGQGRRIV 130 140 150 160 170 180 EEYKRKAKEQ ENIRFYLLFF VFFIVAMAVF MSRGDPNTLL LGVFVILIAL MVTANMRFRT 190 200 210 220 230 240 QAMVPKLLVD NSGRKRAPFV DATGAHAGAL LGDVRHDPFQ CFSGEETVVI RENGEVKVLR 250 260 270 280 290 300 LKDFVEKALE KPSGEGLDGD VKVVYHDFRN ENVEVLTKDG FTKLLYANKR IGKQKLRRVV 310 320 330 340 350 360 NLEKDYWFAL TPDHKVYTTD GLKEAGEITE KDELISVPIT VFDCEDEDLK KIGLLPLTSD 370 380 390 400 410 420 DERLRKIATL MGILFNGGSI DEGLGVLTLK SERSVIEKFV ITLKELFGKF EYEIIKEENT 430 440 450 460 470 480 ILKTRDPRII KFLVGLGAPI EGKDLKMPWW VKLKPSLFLA FLEGFRAHIV EQLVDDPNKN 490 500 510 520 530 540 LPFFQELSWY LGLFGIKADI KVEEVGDKHK IIFDAGRLDV DKQFIETWED VEVTYNLTTE 550 560 570 580 590 600 KGNLLANGLF VKNSGGLGTP AHLRVEPGMI HRAHKGVLFI DEIATLSLKM QQSLLTAMQE 610 620 630 640 650 660 KKFPITGQSE LSSGAMVRTE PVPCDFILVA AGNLDTIEKM HPALRSRIRG YGYEVYMRTT 670 680 690 700 710 720 MPDTVENRRK LVQFVAQEVK KDGRIPHFTR DAVEEIIREA QRRAGRKGHL TLRLRDLGGV 730 740 750 760 770 780 VRAAGDIAVR KGKKYVTRED VLEALQMAKP LEKQLADWYI ERKKEYQVIR TEGGEIGRVN 790 800 810 820 830 840 GLAIIGEQSG IVLPIEAIVA PAASKEEGKI IVTGKLGEIA REAVLNVSAI IKRYKGEDIS 850 860 870 880 890 900 RYDIHVQFLQ TYEGVEGDSA SISVATAVIS ALEEIPVRQD VAMTGSLSVR GEVLPVGGVT 910 920 930 940 950 960 PKIEAAIEAG IKKVIIPKAN EKDVFLSPDK REKIEIIPVE RIDEVLEVAL VESEKKKELI 970 980 990 KRIRETLPLG VSESAGSETL HEHGRDSGSA LSVEESKA

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ248288 Genomic DNA. Translation: CAB50486.1.
PIR	H75005.
RefSeq	NP_127256.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S16.005.
Genome annotation databases
GeneID	1495869.
GenomeReviews	Gene locus PYRAB15820 in contig AL096836_GR.
KEGG	pab:PAB1313.
NMPDR	fig|272844.1.peg.1679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9UYC6.
OMA	ELSSGAM
Enzyme and pathway databases
BioCyc	PABY272844:PAB1313-MON.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR011704. ATPase_AAA-5. IPR003587. Hedgehog_hint_N. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR004663. Pept_S16_Archaea. IPR008269. Pept_S16_C. IPR001984. Peptidase_S16_C. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002078. RNA_pol_sigma_54_int. [Graphical view]
Pfam	PF07728. AAA_5. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR00764. lon_rel. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS01046. LON_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LONH_PYRAB

Accession

Primary (citable) accession number: Q9UYC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)