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Protein

KEOPS complex subunit Bud32

Gene

bud32

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Bud32 has ATPase activity in the context of the KEOPS complex and likely plays a supporting role to the catalytic subunit Kae1.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271ATPPROSITE-ProRule annotation
Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi4 – 129ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATPase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
KEOPS complex subunit Bud32 (EC:3.6.-.-)
Alternative name(s):
Atypical serine/threonine protein kinase Bud32 (EC:2.7.11.1)
Gene namesi
Name:bud32
Ordered Locus Names:PAB1047, PYRAB15890
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810 Componenti: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. EKC/KEOPS complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi127 – 1271D → R: Completely abolishes ATPase activity of the KEOPS complex and completely impairs the tRNA modification activity of the complex. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220KEOPS complex subunit Bud32PRO_0000430322Add
BLAST

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.2 Publications

Protein-protein interaction databases

STRINGi272844.PAB1047.

Structurei

3D structure databases

ProteinModelPortaliQ9UYB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 220220Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG3642.
HOGENOMiHOG000226425.
KOiK07174.
OMAiPPLEQLN.

Family and domain databases

InterProiIPR022495. Bud32.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERiPTHR12209. PTHR12209. 1 hit.
PfamiPF01163. RIO1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UYB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIKQGAEA KIYLAEFSEL YFDYPIKVIV KERIKKRYRI PEIDLKLRKE
60 70 80 90 100
RTIREARILR RAKEFGVNVP YVFEVDTKNM IIVMEYIEGE RLKELLEKLP
110 120 130 140 150
MEERLKVCRE VGRQIGKLHE AGIVHGDLTT SNMILREGKV YFIDFGLAEF
160 170 180 190 200
DDTIEAQGVD LHLLKRAMES THYKWFERGF EEVLKGYIEI RGEDKGREIR
210 220
EKIREIELRG RYRERSWITQ
Length:220
Mass (Da):26,343
Last modified:April 30, 2000 - v1
Checksum:iA37A9382419FEF75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50493.1.
HE613800 Genomic DNA. Translation: CCE71048.1.
PIRiG75006.
RefSeqiNP_127263.1. NC_000868.1.
WP_010868707.1. NC_000868.1.

Genome annotation databases

GeneIDi1495877.
KEGGipab:PAB1047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50493.1.
HE613800 Genomic DNA. Translation: CCE71048.1.
PIRiG75006.
RefSeqiNP_127263.1. NC_000868.1.
WP_010868707.1. NC_000868.1.

3D structure databases

ProteinModelPortaliQ9UYB9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB1047.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1495877.
KEGGipab:PAB1047.

Phylogenomic databases

eggNOGiCOG3642.
HOGENOMiHOG000226425.
KOiK07174.
OMAiPPLEQLN.

Family and domain databases

InterProiIPR022495. Bud32.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR018934. RIO_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PANTHERiPTHR12209. PTHR12209. 1 hit.
PfamiPF01163. RIO1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.
  3. "In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya."
    Perrochia L., Crozat E., Hecker A., Zhang W., Bareille J., Collinet B., van Tilbeurgh H., Forterre P., Basta T.
    Nucleic Acids Res. 41:1953-1964(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN T(6)A TRNA MODIFICATION, SUBUNIT.
  4. "Functional assignment of KEOPS/EKC complex subunits in the biosynthesis of the universal t6A tRNA modification."
    Perrochia L., Guetta D., Hecker A., Forterre P., Basta T.
    Nucleic Acids Res. 41:9484-9499(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE KEOPS COMPLEX, CATALYTIC ACTIVITY, SUBUNIT, MUTAGENESIS OF ASP-127.

Entry informationi

Entry nameiBUD32_PYRAB
AccessioniPrimary (citable) accession number: Q9UYB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 2014
Last sequence update: April 30, 2000
Last modified: March 31, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is considered an atypical serine/threonine kinase, because it lacks the conventional structural elements necessary for the substrate recognition as well as a lysine residue that in all other serine/threonine kinases participates in the catalytic event (By similarity). Bud32 has protein kinase activity in vitro, but in the context of the KEOPS complex, the catalytic subunit Kae1 switches the activity of Bud32 from kinase into ATPase (PubMed:23945934).By similarity1 Publication

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.