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Protein

KEOPS complex subunit Bud32

Gene

bud32

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Bud32 has ATPase activity in the context of the KEOPS complex and likely plays a supporting role to the catalytic subunit Kae1.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27ATPPROSITE-ProRule annotation1
Active sitei127Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi4 – 12ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • tRNA threonylcarbamoyladenosine modification Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Kinase, Serine/threonine-protein kinase, Transferase
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPABY272844:G1GT8-1752-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
KEOPS complex subunit Bud32 (EC:3.6.-.-1 Publication)
Alternative name(s):
Atypical serine/threonine protein kinase Bud32 (EC:2.7.11.11 Publication)
Gene namesi
Name:bud32
Ordered Locus Names:PAB1047, PYRAB15890
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • EKC/KEOPS complex Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi127D → R: Completely abolishes ATPase activity of the KEOPS complex and completely impairs the tRNA modification activity of the complex. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004303221 – 220KEOPS complex subunit Bud32Add BLAST220

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.2 Publications

Protein-protein interaction databases

STRINGi272844.PAB1047

Structurei

3D structure databases

ProteinModelPortaliQ9UYB9
SMRiQ9UYB9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 220Protein kinasePROSITE-ProRule annotationAdd BLAST220

Domaini

This protein is considered an atypical serine/threonine kinase, because it lacks the conventional structural elements necessary for the substrate recognition as well as a lysine residue that in all other serine/threonine kinases participates in the catalytic event (By similarity). Bud32 has protein kinase activity in vitro, but in the context of the KEOPS complex, the catalytic subunit Kae1 switches the activity of Bud32 from kinase into ATPase (PubMed:23945934).By similarity1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01185 Archaea
COG3642 LUCA
HOGENOMiHOG000226425
KOiK08851
OMAiHKLYMEY
OrthoDBiPOG093Z09WH

Family and domain databases

InterProiView protein in InterPro
IPR022495 Bud32
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008266 Tyr_kinase_AS
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
TIGRFAMsiTIGR03724 arch_bud32, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UYB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIKQGAEA KIYLAEFSEL YFDYPIKVIV KERIKKRYRI PEIDLKLRKE
60 70 80 90 100
RTIREARILR RAKEFGVNVP YVFEVDTKNM IIVMEYIEGE RLKELLEKLP
110 120 130 140 150
MEERLKVCRE VGRQIGKLHE AGIVHGDLTT SNMILREGKV YFIDFGLAEF
160 170 180 190 200
DDTIEAQGVD LHLLKRAMES THYKWFERGF EEVLKGYIEI RGEDKGREIR
210 220
EKIREIELRG RYRERSWITQ
Length:220
Mass (Da):26,343
Last modified:May 1, 2000 - v1
Checksum:iA37A9382419FEF75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA Translation: CAB50493.1
HE613800 Genomic DNA Translation: CCE71048.1
PIRiG75006
RefSeqiWP_010868707.1, NC_000868.1

Genome annotation databases

EnsemblBacteriaiCAB50493; CAB50493; PAB1047
GeneIDi1495877
KEGGipab:PAB1047
PATRICifig|272844.11.peg.1695

Similar proteinsi

Entry informationi

Entry nameiBUD32_PYRAB
AccessioniPrimary (citable) accession number: Q9UYB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 2014
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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