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Protein

Glutamine synthetase

Gene

glnA

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Magnesium 1By similarity1
Metal bindingi124Magnesium 2By similarity1
Binding sitei172ATPBy similarity1
Metal bindingi177Magnesium 2By similarity1
Metal bindingi184Magnesium 2By similarity1
Binding sitei229L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi233Magnesium 1; via pros nitrogenBy similarity1
Binding sitei237ATPBy similarity1
Binding sitei283L-glutamateBy similarity1
Binding sitei289L-glutamateBy similarity1
Binding sitei301ATPBy similarity1
Binding sitei301L-glutamateBy similarity1
Binding sitei306ATPBy similarity1
Metal bindingi318Magnesium 1By similarity1
Binding sitei320L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi235 – 237ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:PYRAB16090
ORF Names:PAB1292
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532081 – 439Glutamine synthetaseAdd BLAST439

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Protein-protein interaction databases

STRINGi272844.PAB1292.

Structurei

3D structure databases

ProteinModelPortaliQ9UY99.
SMRiQ9UY99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
HOGENOMiHOG000005156.
KOiK01915.
OMAiIEAAWNT.
OrthoDBiPOG093Z00GE.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UY99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVSEVKSGA TRSPKFVQLI FVDINGMPKG MEIPASRLQE AIEDGISFDG
60 70 80 90 100
SSVPGFQGIE DSDLIFKADP DTYVEVPWDN VARVYGYIYK DGKPYGADPR
110 120 130 140 150
GVLKRVIEKL AEMGIKAYIG PEPEFYLFKK NGSWELEIPD VGGYFDILTL
160 170 180 190 200
DKAKDIKREI AEYMPSFGLV PEVLHHEVGK AQHEIDFRYD EALKTADNII
210 220 230 240 250
SFKYIVKAVA EVHGLYATFM PKPIYGMPGN GMHLHISLWK EGENIFKGEE
260 270 280 290 300
GLSETALHFI GGLLKHAKAL TAITNPTVNS YKRLVPGYEA PVYISWGYKN
310 320 330 340 350
RSALIRVPAF WGNGARIEYR CPDPSANPYF AFAAILMAGL DGIKHKVEPF
360 370 380 390 400
AYVEENVYEM DEGKRKELGI DTLPGSLGEA LDELEKDKVV REALGEAYKN
410 420 430
FIEYKRKEWE SYLEYLEAKH LPKDTKRVTE WELERYFFI
Length:439
Mass (Da):49,743
Last modified:May 1, 2000 - v1
Checksum:i62CCFD3970A98AF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50513.1.
HE613800 Genomic DNA. Translation: CCE71069.1.
PIRiC75009.
RefSeqiWP_010868727.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB50513; CAB50513; PAB1292.
GeneIDi1495898.
KEGGipab:PAB1292.
PATRICifig|272844.11.peg.1718.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1A_PYRAB
AccessioniPrimary (citable) accession number: Q9UY99
Secondary accession number(s): G8ZJW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: June 7, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families