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Q9UY71 (PUR2_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:PYRAB16370
ORF Names:PAB1271
OrganismPyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]
Taxonomic identifier272844 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151514

Regions

Domain108 – 316209ATP-grasp
Nucleotide binding135 – 19460ATP By similarity

Sites

Metal binding2741Magnesium or manganese 1 By similarity
Metal binding2861Magnesium or manganese 1 By similarity
Metal binding2861Magnesium or manganese 2 By similarity
Metal binding2881Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9UY71 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: EBE04A02E06EC18E

FASTA43848,748
        10         20         30         40         50         60 
MRVLLVGGGG REHAIGEALA RGGAELYVVS KHKNPGLARI SRDYGISKET DVEKVVRFAR 

        70         80         90        100        110        120 
MWNVDLAFIG PEAPLEAGIV NALEREGIPT VGPTKEAARL ETNKAWAREF MERNDIPGRK 

       130        140        150        160        170        180 
LFKVFDDVEE MKAWIDEYGK PVVVKPLGLT GGKGVKVVGY QLKDNEEAKE YAEYLIKKDG 

       190        200        210        220        230        240 
KVLIEERTDG VEFTFQVFSD GRNVIPMPLV QDYPHAYEGD VGPITGGMGS YSCSNHILPF 

       250        260        270        280        290        300 
ITKEDWKMAL RTLEETIKAM RKEGYPYKGI LYGQFMLSRE GPVIIEYNAR FGDPEAINVL 

       310        320        330        340        350        360 
SILEDNLVEI AEGIVKGRVR GAKFQEKATV VKYLAPKGYP ENPIRGAEIM VNEEAIKEEG 

       370        380        390        400        410        420 
AKIVYASVDE NMKLLGSRAL AMVGVADTLE EAEKIAEAGI RHVRGPVFYR KDVGTKESIE 

       430 
KRIRTMKELG KEFEPNSC 

« Hide

References

« Hide 'large scale' references
[1]"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi."
Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P.
Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GE5 / Orsay.
[2]"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
Gao J., Wang J.
Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: GE5 / Orsay.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ248288 Genomic DNA. Translation: CAB50541.1.
HE613800 Genomic DNA. Translation: CCE71098.1.
PIRG75012.
RefSeqNP_127311.1. NC_000868.1.

3D structure databases

ProteinModelPortalQ9UY71.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272844.PAB1271.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB50541; CAB50541; PAB1271.
GeneID1495927.
KEGGpab:PAB1271.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033464.
KOK01945.
OMAYKGFLYA.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_PYRAB
AccessionPrimary (citable) accession number: Q9UY71
Secondary accession number(s): G8ZJZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways