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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831SubstrateUniRule annotation
Binding sitei219 – 2191Substrate; via amide nitrogenUniRule annotation
Metal bindingi226 – 2261ManganeseUniRule annotation
Binding sitei226 – 2261SubstrateUniRule annotation
Metal bindingi245 – 2451Manganese; via tele nitrogenUniRule annotation
Binding sitei267 – 2671SubstrateUniRule annotation
Active sitei269 – 2691UniRule annotation
Metal bindingi286 – 2861ManganeseUniRule annotation
Binding sitei383 – 3831GTPUniRule annotation
Binding sitei415 – 4151GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:PYRAB16550
ORF Names:PAB1253
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 618618Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103617Add
BLAST

Proteomic databases

PRIDEiQ9UY53.

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB1253.

Structurei

3D structure databases

ProteinModelPortaliQ9UY53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni381 – 3833Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UY53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKLKRFLPE DQYEKLAAIN NPYLHEFLAE WIEWLKPSKV FVCTDSPEDE
60 70 80 90 100
EYVRWKALYY GEEKMLEMPR HTVHYDNYYD QARDKANTKL LVPKGVTLPF
110 120 130 140 150
LNTMDREEGL KEIREIMKGI MKGKELFICF FVLGPRNSIF TIPAVQLTDS
160 170 180 190 200
AYVAHSEFLL YRKGYEEFKR LGPTKNFLKF VHSAGELDER KTSKNLDKRR
210 220 230 240 250
IYIDLVDETV YSANTQYGGN TIGLKKLAFR LTIQRAVREG WLSEHMFLMR
260 270 280 290 300
VNGPNGRKTY FTGAYPSMCG KTSTAMIPWE NIVGDDLVFI KNVDGTARAV
310 320 330 340 350
NVEIGVFGII EGINEKDDPI IWQVLHSPVE IIFSNVLVKD GKPYWNGMGI
360 370 380 390 400
EIPDEGENHS GKWWRGKRDK EGNEIPPSHK NARFTVRLEA FPNLDKEALE
410 420 430 440 450
NPCGVEVGGM IFGGRDPDTW PPVREAFDWE HGVITMGASL ESETTAATLG
460 470 480 490 500
KEGVRAFNPM SILDFLSVHL GDYIRNYLEF GRKLKKKPKI FAVNYFLREN
510 520 530 540 550
GKWLNEKLDK AVWLKWMELR VHGDVEAIET PIGYIPKYED LRELFKQVLN
560 570 580 590 600
KEYKKEDYEK QFKIRVPELL AKIDRIWNIY EPIGNIPEEL FKQLEEERER
610
LLKAREKYGD YISPFSLL
Length:618
Mass (Da):71,880
Last modified:May 1, 2000 - v1
Checksum:i88E045DC671956E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50559.1.
HE613800 Genomic DNA. Translation: CCE71123.1.
PIRiA75015.
RefSeqiNP_127329.1. NC_000868.1.
WP_010868773.1. NC_000868.1.

Genome annotation databases

EnsemblBacteriaiCAB50559; CAB50559; PAB1253.
GeneIDi1495952.
KEGGipab:PAB1253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50559.1.
HE613800 Genomic DNA. Translation: CCE71123.1.
PIRiA75015.
RefSeqiNP_127329.1. NC_000868.1.
WP_010868773.1. NC_000868.1.

3D structure databases

ProteinModelPortaliQ9UY53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB1253.

Proteomic databases

PRIDEiQ9UY53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB50559; CAB50559; PAB1253.
GeneIDi1495952.
KEGGipab:PAB1253.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiPCKG_PYRAB
AccessioniPrimary (citable) accession number: Q9UY53
Secondary accession number(s): G8ZK16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: May 27, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.