Q9UY36 (SYA_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	PYRAB16720
ORF Names:	PAB1245

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	914 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 914	914	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000075271
Sites
	Metal binding	613	1	Zinc By similarity
	Metal binding	617	1	Zinc By similarity
	Metal binding	717	1	Zinc By similarity
	Metal binding	721	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UY36 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C4B449FE9E03B4E1	FASTA	914	104,858
	10 20 30 40 50 60 MEFIMKTRMF EEEGWIRKKC KVCGKPFWTL DPDRETCGDP PCDEYQFIGK PGIPKKYTLD 70 80 90 100 110 120 EMREKFLKFF EKHEVYPHGR VKRYPVLPRW RDDVLLVGAS IMDFQPWVIS GEADPPANPL 130 140 150 160 170 180 VISQPSIRFT DIDNVGITGR HFTIFEMMAH HAFNYPGKPI YWIDETVELA FEFFTKELKM 190 200 210 220 230 240 KPEDITFKEN PWAGGGNAGP AFEVLYRGLE VATLVFMQYK KAPENAPEDQ VVIIKGDRYV 250 260 270 280 290 300 PMETKVVDTG YGLERLVWMS QGTPTAYDAV LGYVVEPLKR MAGVEKIDER ILMENSRLAG 310 320 330 340 350 360 MFDIEDMGDL KLLRKKVAEK VGISVEELEK AIRPYELIYA IADHTKALTF MLADGVIPSN 370 380 390 400 410 420 VKAGYLARLL IRKSIRHLKE LGLEVPLSEI VALHIKELHK TFPEFKEMED VILEIIDLEE 430 440 450 460 470 480 KKYSETLKRG SDLVRREIAK LKKKGMSEIP LEKLITFYES HGLTPELVKE IAEKEGIKVH 490 500 510 520 530 540 VPDNFYSIVA KEAEKEKEEK EEEVVDFELV KDLPETRTLY YEDPFMKEFE ARVLRVIGDW 550 560 570 580 590 600 IVLDQTAFYP EGGGQPYDTG VLFVNGSEVK VTNVQKVGKV IVHKVENPGL FKEGMEVRGR 610 620 630 640 650 660 IDWDRRIQHM RHHTGTHVLM GALVRVLGKH VWQAGSQLTT DWARLDISHY KRISDEELRE 670 680 690 700 710 720 IERLANRIVM EDRKVTWEWL PRTEAEQRYG FRLYQGGVVP GRVIRVVKIE DWDVQACGGT 730 740 750 760 770 780 HLPSTGLVGP IKILRTERIQ DGVERIIFAC GEAAIKEWQK EREILKKASQ VLRVPPEKLP 790 800 810 820 830 840 ETAERFFNEW KEARKEVEKL KKELAKLLVY ELEAKVQKVK EYEFIGEIVE GSMDDLREAV 850 860 870 880 890 900 ERLKKPNRIV VLVSKEGYFA ISVGDNVNLN ANDLAKKLTS IAGGGGGGRK DVAQGRIKDV 910 SKAKEAIESI VKLL

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248288 Genomic DNA. Translation: CAB50576.1.
PIR	B75017.
RefSeq	NP_127346.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UY36.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000003041; EBPYRP00000002972; EBPYRG00000003041.
GeneID	1495969.
GenomeReviews	Gene locus PYRAB16720 in contig AL096836_GR.
KEGG	pab:PAB1245.
NMPDR	fig|272844.1.peg.1779.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022658.
HOGENOM	HBG392147.
OMA	MFTNSGM.
PhylomeDB	Q9UY36.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	PABY272844:PAB1245-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_PYRAB

Accession

Primary (citable) accession number: Q9UY36

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program