Q9UY11 (SYW_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	PYRAB16970
ORF Names:	PAB1111

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus abyssi

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Tryptophan--tRNA ligase HAMAP-Rule MF_00140		PRO_0000136727
Regions
	Motif	82 – 90	9	"HIGH" region HAMAP-Rule MF_00140
	Motif	253 – 257	5	"KMSKS" region HAMAP-Rule MF_00140

Sequences

	Sequence		Length	Mass (Da)
	Q9UY11 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 4C29D01414976B12	FASTA	385	45,100
	10 20 30 40 50 60 MVEDFKVTPW EVEGVVDYNK LIEHFGTSPL TEELLEKTAE LTKSELPLFF RRKFFFSHRD 70 80 90 100 110 120 YDKVLQDYEE GRGFFLYTGR GPSGPMHIGH IIPFFATKWL QEKFGVNLYI QITDDEKFLF 130 140 150 160 170 180 KENLTFEDTK HWAYENILDI IAVGFDPDKT FIFQNSEFTK IYEMAIPIAK KINFSMAKAV 190 200 210 220 230 240 FGFTEQSKIG MIFFPAIQIA PTFFEKRRCL IPAAIDQDPY WRLQRDFAES LGYYKTAAIH 250 260 270 280 290 300 SKFVPSLTSL SGKMSASKPE TAIYLTDSPE DVEKKVWKFA LTGGRPTLKE QREKGGEPEK 310 320 330 340 350 360 CVVFKWLEIF FEEDDKKLKE RYYACKNGEL TCGECKRYLI SKIQEFLKEH QKRRKKAEKQ 370 380 IEKFKYTGKL AQEMWDKAIP EPLKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.
[2]	"Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638." Gao J., Wang J. Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248288 Genomic DNA. Translation: CAB50601.1. HE613800 Genomic DNA. Translation: CCE71167.1.
PIR	C75020.
RefSeq	NP_127372.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UY11.
ModBase	Search...
Protein-protein interaction databases
STRING	272844.PAB1111.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB50601; CAB50601; PAB1111.
GeneID	1495996.
KEGG	pab:PAB1111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000224742.
KO	K01867.
OMA	KTGGRET.
ProtClustDB	PRK12285.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_A. Trp_tRNA_synth_A.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR020653. Tryptophan-tRNA-ligase_arc. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_PYRAB

Accession

Primary (citable) accession number: Q9UY11
Secondary accession number(s): G8ZK60

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program