Q9UXW6 (PURL_PYRAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	PYRAB17420
ORF Names:	PAB1201

Organism

Pyrococcus abyssi (strain GE5 / Orsay) [Complete proteome] [HAMAP]

Taxonomic identifier

272844 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100519
Regions
	Nucleotide binding	89 – 100	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9UXW6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8D75A4AEEB102499	FASTA	705	77,867
	10 20 30 40 50 60 MLPREEKIIR ERLGREPNEV EKAMLEVMWS EHVSYKSSRK WLKLLPTKNE HVILGPGEDA 70 80 90 100 110 120 GVVKFDESTW IVIGIESHNH PSAVEPYGGA ATGIGGIVRD ILCMGARPIA LLDPIRFGPL 130 140 150 160 170 180 EKEKNRYLFE YVVKGIADYG NRIGVPTVGG ETEFDESLDN YTLVNVVCVG IMKPEHLVHS 190 200 210 220 230 240 YVTKPGLKLV LVGNRTGRDG IHGVTFASEE LSENAEEEDR SAVQIPDPFT EKLLIEATLE 250 260 270 280 290 300 AVYTGKVKAL KDLGGGGLTC AASEMAGKKG LGAVIYADRV PLREPGMTPL EVMISESQER 310 320 330 340 350 360 MLFAVEPEDV EELAKIFEKY ELEWAVVGEI IEEPRFVVYW KGDKVADLPI ELLTNVPTIE 370 380 390 400 410 420 WPMKEYKLEE DVETPDIALS KAFDLVWSSP NIVAKRWVWE QYDHEVQGRT VVKPGFDAAV 430 440 450 460 470 480 LKINGEYGLA ITSDGNPSYC YLNPYHGAMG TVAEVVRNLV SVGAKPLALV DNLNFASPER 490 500 510 520 530 540 PEVYWSFVET VKGLADAAKA FDLAYVSGNV SFYNEVVDRP VKPTPVVAGI GKVKLKDIPR 550 560 570 580 590 600 GPRDGDVIAL IGSTRRELGG SELYRVLGIK GGIAPRVNLE EEKGNALAIL NLIENDLVTF 610 620 630 640 650 660 VHDVSRGGVA VALAELSAWF NVGVKAKFTS SFKSIDFAFS ESHGRYIITL PEDKVEEAKE 670 680 690 700 IAKISIVGRV GGDNFALEVN GEKVEKDIEE LSRIYWNYMY DLLEL

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ248288 Genomic DNA. Translation: CAB50647.1.
PIR	A75026.
RefSeq	NP_127418.1. NC_000868.1.
3D structure databases
ProteinModelPortal	Q9UXW6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBPYRT00000002204; EBPYRP00000002135; EBPYRG00000002204.
GeneID	1496044.
GenomeReviews	Gene locus PYRAB17420 in contig AL096836_GR.
KEGG	pab:PAB1201.
NMPDR	fig|272844.1.peg.1854.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000022555.
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q9UXW6.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	PABY272844:PAB1201-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PYRAB

Accession

Primary (citable) accession number: Q9UXW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program