Reviewed, UniProtKB/Swiss-Prot Q9UXU7 (VATA_PYRAB)

Names and origin

Protein names

Recommended name:
    V-type ATP synthase alpha chain
    EC=3.6.3.14
Alternative name(s):
    V-ATPase subunit A
Cleaved into the following chain:
    1- Recommended name:
            Pab atpA intein
        Alternative name(s):
            Pab VMA intein

Gene names

Name:	atpA
Ordered Locus Names:	PYRAB17610
ORF Names:	PAB2378

Organism

Pyrococcus abyssi [Complete proteome] [HAMAP]

Taxonomic identifier

29292 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	1017 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit. HAMAP MF_00309
Catalytic activity	ATP + H2O + H+(In) = ADP + phosphate + H+(Out). HAMAP MF_00309
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation Potential.
Miscellaneous	The intein interrupts the ATP-binding site. HAMAP MF_00309
Sequence similarities	Belongs to the ATPase alpha/beta chains family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro plasma membrane ATP synthesis coupled proton transport Inferred from electronic annotation. Source: HAMAP
Cellular component	proton-transporting two-sector ATPase complex, catalytic domain Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: InterPro hydrogen ion transporting ATP synthase activity, rotational mechanism Inferred from electronic annotation. Source: HAMAP proton-transporting ATPase activity, rotational mechanism Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	V-type ATP synthase alpha chain, 1st part Potential		PRO_0000002461
	Chain	241 – 669	429	Pab atpA intein Potential		PRO_0000002462
	Chain	670 – 1017	348	V-type ATP synthase alpha chain, 2nd part Potential		PRO_0000002463
Regions
	Domain	396 – 529	134	DOD-type homing endonuclease

Sequences

	Sequence		Length	Mass (Da)
	Q9UXU7-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 0F2008895F3E407A	FASTA	1,017	114,354
	10 20 30 40 50 60 MVAKGRIIRV TGPLVVADGM KGAKMYEVVR VGELGLIGEI IRLEGDKAVI QVYEETAGVR 70 80 90 100 110 120 PGEPVIGTGS SLSVELGPGL LTSIYDGIQR PLEVIREKTG DFIARGVTAP ALPRDKKWHF 130 140 150 160 170 180 IPKVKVGDKV VGGDIIGEVP ETSIITHKIM VPPGIEGEIV EIAEEGEYTI EEVIAKVKTP 190 200 210 220 230 240 SGEIKELKMY QRWPVRVKRP YKEKLPPEVP LITGQRVIDT FFPQAKGGTA AIPGPFGSGK 250 260 270 280 290 300 CVDGDTLVLT KEFGLIKIKD LYKILDGKGK KTVNGNEEWT ELERPITLYG YKDGKIVEIK 310 320 330 340 350 360 ATHVYKGFSA GMIEIRTRTG RKIKVTPIHK LFTGRVTKNG LEIREVMAKD LKKGDRIIVA 370 380 390 400 410 420 KKIDGGERVK LNIRVEQKRG KKIRIPDVLD EKLAEFLGYL IADGTLKPRT VAIYNNDESL 430 440 450 460 470 480 LRRANELANE LFNIEGKIVK GRTVKALLIH SKALVEFFSK LGVPRNKKAR TWKVPKELLI 490 500 510 520 530 540 SEPEVVKAFI KAYIMCDGYY DENKGEIEIV TASEEAAYGF SYLLAKLGIY AIIREKIIGD 550 560 570 580 590 600 KVYYRVVISG ESNLEKLGIE RVGRGYTSYD IVPVEVEELY NALGRPYAEL KRAGIEIHNY 610 620 630 640 650 660 LSGENMSYEM FRKFAKFVGM EEIAENHLTH VLFDEIVEIR YISEGQEVYD VTTETHNFIG 670 680 690 700 710 720 GNMPTLLHNT VTQHQLAKWS DAQVVIYIGC GERGNEMTDV LEEFPKLKDP KTGKPLMERT 730 740 750 760 770 780 VLIANTSNMP VAAREASIYT GITIAEYFRD MGYDVALMAD STSRWAEALR EISGRLEEMP 790 800 810 820 830 840 GEEGYPAYLA SKLAEFYERA GRVVTLGSDY RVGSVSVIGA VSPPGGDFSE PVVQNTLRVV 850 860 870 880 890 900 KVFWALDADL ARRRHFPAIN WLTSYSLYVD AVKDWWHKNV DPEWKAMRDK AMELLQKESE 910 920 930 940 950 960 LQEIVRIVGP DALPERERAI LLVARMLRED YLQQDAFDEV DTYCPPEKQV TMMRVLLNFY 970 980 990 1000 1010 DKTMEAISRG VPLEEIAKLP VREEIGRMKF EPDVGKIKAL IDKTNEQFEE LFKKYGA

References

[1]

"An integrated analysis of the genome of the hyperthermophilic archaeon Pyrococcus abyssi." Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O., Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J., Weissenbach J., Zivanovic Y., Forterre P. Mol. Microbiol. 47:1495-1512(2003) [PubMed: 12622808] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GE5 / Orsay.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ248288 Genomic DNA. Translation: CAB50666.1.
PIR	D75028.
RefSeq	NP_127437.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1496064.
GenomeReviews	Gene locus PYRAB17610 in contig AL096836_GR.
KEGG	pab:PAB2378.
NMPDR	fig|272844.1.peg.1874.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9UXU7.
OMA	HNCGERG
Enzyme and pathway databases
BioCyc	PABY272844:PAB2378-MON.
BRENDA	3.6.3.14. 262861.
Family and domain databases
HAMAP	MF_00309. Divergent sequence. [Tree]
InterPro	IPR018118. ATPase_F1/A1-cplx_a/bsu_N. IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C. IPR004100. ATPase_F1/V1/A1-cplx_a/bsu_N. IPR020003. ATPase_F1/V1/A1_a/bsu_AS. IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. [Graphical view]
Pfam	PF00006. ATP-synt_ab. 1 hit. PF00306. ATP-synt_ab_C. 1 hit. PF02874. ATP-synt_ab_N. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS00152. ATPASE_ALPHA_BETA. 1 hit. PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VATA_PYRAB

Accession

Primary (citable) accession number: Q9UXU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)