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Protein

V-type ATP synthase alpha chain

Gene

atpA

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. endonuclease activity Source: InterPro
  3. hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances Source: InterPro

GO - Biological processi

  1. ATP biosynthetic process Source: UniProtKB-KW
  2. ATP hydrolysis coupled proton transport Source: InterPro
  3. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
V-type ATP synthase alpha chain (EC:3.6.3.14)
Alternative name(s):
V-ATPase subunit A
Cleaved into the following chain:
Alternative name(s):
Pab VMA intein
Gene namesi
Name:atpA
Ordered Locus Names:PYRAB17610
ORF Names:PAB2378
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000000810 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. proton-transporting two-sector ATPase complex, catalytic domain Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240V-type ATP synthase alpha chain, 1st partSequence AnalysisPRO_0000002461Add
BLAST
Chaini241 – 669429Pab AtpA inteinSequence AnalysisPRO_0000002462Add
BLAST
Chaini670 – 1017348V-type ATP synthase alpha chain, 2nd partSequence AnalysisPRO_0000002463Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB2378.

Structurei

3D structure databases

ProteinModelPortaliQ9UXU7.
SMRiQ9UXU7. Positions 60-287, 670-1017.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini396 – 529134DOD-type homing endonucleaseAdd
BLAST

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000161057.
KOiK02117.
OMAiVHNCGER.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
2.170.16.10. 3 hits.
3.10.28.10. 2 hits.
3.40.50.300. 3 hits.
HAMAPiMF_00309. ATP_synth_A_arch.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 2 hits.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9UXU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAKGRIIRV TGPLVVADGM KGAKMYEVVR VGELGLIGEI IRLEGDKAVI
60 70 80 90 100
QVYEETAGVR PGEPVIGTGS SLSVELGPGL LTSIYDGIQR PLEVIREKTG
110 120 130 140 150
DFIARGVTAP ALPRDKKWHF IPKVKVGDKV VGGDIIGEVP ETSIITHKIM
160 170 180 190 200
VPPGIEGEIV EIAEEGEYTI EEVIAKVKTP SGEIKELKMY QRWPVRVKRP
210 220 230 240 250
YKEKLPPEVP LITGQRVIDT FFPQAKGGTA AIPGPFGSGK CVDGDTLVLT
260 270 280 290 300
KEFGLIKIKD LYKILDGKGK KTVNGNEEWT ELERPITLYG YKDGKIVEIK
310 320 330 340 350
ATHVYKGFSA GMIEIRTRTG RKIKVTPIHK LFTGRVTKNG LEIREVMAKD
360 370 380 390 400
LKKGDRIIVA KKIDGGERVK LNIRVEQKRG KKIRIPDVLD EKLAEFLGYL
410 420 430 440 450
IADGTLKPRT VAIYNNDESL LRRANELANE LFNIEGKIVK GRTVKALLIH
460 470 480 490 500
SKALVEFFSK LGVPRNKKAR TWKVPKELLI SEPEVVKAFI KAYIMCDGYY
510 520 530 540 550
DENKGEIEIV TASEEAAYGF SYLLAKLGIY AIIREKIIGD KVYYRVVISG
560 570 580 590 600
ESNLEKLGIE RVGRGYTSYD IVPVEVEELY NALGRPYAEL KRAGIEIHNY
610 620 630 640 650
LSGENMSYEM FRKFAKFVGM EEIAENHLTH VLFDEIVEIR YISEGQEVYD
660 670 680 690 700
VTTETHNFIG GNMPTLLHNT VTQHQLAKWS DAQVVIYIGC GERGNEMTDV
710 720 730 740 750
LEEFPKLKDP KTGKPLMERT VLIANTSNMP VAAREASIYT GITIAEYFRD
760 770 780 790 800
MGYDVALMAD STSRWAEALR EISGRLEEMP GEEGYPAYLA SKLAEFYERA
810 820 830 840 850
GRVVTLGSDY RVGSVSVIGA VSPPGGDFSE PVVQNTLRVV KVFWALDADL
860 870 880 890 900
ARRRHFPAIN WLTSYSLYVD AVKDWWHKNV DPEWKAMRDK AMELLQKESE
910 920 930 940 950
LQEIVRIVGP DALPERERAI LLVARMLRED YLQQDAFDEV DTYCPPEKQV
960 970 980 990 1000
TMMRVLLNFY DKTMEAISRG VPLEEIAKLP VREEIGRMKF EPDVGKIKAL
1010
IDKTNEQFEE LFKKYGA
Length:1,017
Mass (Da):114,354
Last modified:May 1, 2000 - v1
Checksum:i0F2008895F3E407A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50666.1.
HE613800 Genomic DNA. Translation: CCE71235.1.
PIRiD75028.
RefSeqiNP_127437.1. NC_000868.1.
WP_010868880.1. NC_000868.1.

Genome annotation databases

GeneIDi1496064.
KEGGipab:PAB2378.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248288 Genomic DNA. Translation: CAB50666.1.
HE613800 Genomic DNA. Translation: CCE71235.1.
PIRiD75028.
RefSeqiNP_127437.1. NC_000868.1.
WP_010868880.1. NC_000868.1.

3D structure databases

ProteinModelPortaliQ9UXU7.
SMRiQ9UXU7. Positions 60-287, 670-1017.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272844.PAB2378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1496064.
KEGGipab:PAB2378.

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000161057.
KOiK02117.
OMAiVHNCGER.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
2.170.16.10. 3 hits.
3.10.28.10. 2 hits.
3.40.50.300. 3 hits.
HAMAPiMF_00309. ATP_synth_A_arch.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR000793. ATPase_F1/V1/A1-cplx_a/bsu_C.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR024034. ATPase_F1_bsu/V1_C.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR027417. P-loop_NTPase.
IPR022878. V-ATPase_asu.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 2 hits.
PF00306. ATP-synt_ab_C. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF47917. SSF47917. 1 hit.
SSF50615. SSF50615. 1 hit.
SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GE5 / Orsay.
  2. "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and Pyrococcus furiosus DSM 3638."
    Gao J., Wang J.
    Curr. Microbiol. 64:118-129(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: GE5 / Orsay.

Entry informationi

Entry nameiVATA_PYRAB
AccessioniPrimary (citable) accession number: Q9UXU7
Secondary accession number(s): G8ZKU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: April 29, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The intein interrupts the ATP-binding site.

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.