Q9UXP0 (MER_METTI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydromethanopterin reductase
EC=1.5.99.11

Alternative name(s):
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase

Gene names

Name:	mer
Synonyms:	ffdA

Organism

Methanolobus tindarius

Taxonomic identifier

2221 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanolobus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reduction of methylene-H4MPT to methyl-H4MPT By similarity. HAMAP MF_01091
Catalytic activity	5-methyltetrahydromethanopterin + coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420. HAMAP MF_01091
Pathway	One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2. HAMAP MF_01091
Subcellular location	Cytoplasm By similarity HAMAP MF_01091.
Sequence similarities	Belongs to the mer family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	5,10-methylenetetrahydromethanopterin reductase HAMAP MF_01091		PRO_0000084811

Sequences

	Sequence		Length	Mass (Da)
	Q9UXP0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 16F3AB9733A45D82	FASTA	326	34,043
	10 20 30 40 50 60 MTFGIEFVPS DPVLKIAHYA KLAEQQGFDN VWITDHYNNR DVYSTLTVLA MNTNSIKLGT 70 80 90 100 110 120 GVTNPYTRNA AITASSIGSL NEISGGRAIL GLGPGEQATF DAMGISWEQP LTTTKESIAA 130 140 150 160 170 180 IRGFLAGEKV TMDGDMIKFG GAKMAFKAGD VPIYMGAQGP KMLELAGEVS DGVLINASHP 190 200 210 220 230 240 KDFEVAVKQI AAGAKKAGRD PKEVDVAAYA CFSIDKDAAK AKSAAQIVVA FIVAGSPDMV 250 260 270 280 290 300 LERHGIDPAA KADIGGAIAK GDFGALMGGM VTDSMMDAFS ICGTPDDCKA RINELLDIGV 310 320 TQIVAGSPIG PNKEKAIKLI GKEIIG

References

[1]	"The F420H2-dehydrogenase from Methanolobus tindarius: cloning of the ffd operon and expression of the genes in Escherichia coli." Westenberg D.J., Braune A., Ruppert C., Mueller V., Herzberg C., Gottschalk G., Blaut M. FEMS Microbiol. Lett. 170:389-398(1999) [PubMed: 9933933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 2278.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ011519 Genomic DNA. Translation: CAB56639.1.
PIR	T45226.
3D structure databases
ProteinModelPortal	Q9UXP0.
SMR	Q9UXP0. Positions 1-326.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01091. F420_mer. [Tree]
InterPro	IPR011251. Luciferase-like_dom. IPR019946. MeH4methanopterin_reductase. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
SUPFAM	SSF51679. Luciferase_like. 1 hit.
TIGRFAMs	TIGR03555. F420_mer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MER_METTI

Accession

Primary (citable) accession number: Q9UXP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	May 1, 2000
Last modified:	October 19, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program