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Q9UXC2 (RRP41_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exosome complex component Rrp41

EC=3.1.13.-
Gene names
Name:rrp41
Ordered Locus Names:SSO0735
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'->5' exoribonuclease activity. Can also synthesize heteropolymeric RNA-tails. Binds RNA. Ref.5 Ref.8 Ref.9

Subunit structure

Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits. Ref.5 Ref.7 Ref.8 Ref.9 Ref.11

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00591.

Sequence similarities

Belongs to the RNase PH family. Rrp41 subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
Exosome
   Molecular functionExonuclease
Hydrolase
Nuclease
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

exosome (RNase complex)

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionexoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Exosome complex component Rrp41 HAMAP-Rule MF_00591
PRO_0000139991

Experimental info

Mutagenesis981R → E: Abolishes exoribonuclease activity; when associated with E-99. Ref.7
Mutagenesis991R → E: Abolishes exoribonuclease activity; when associated with E-98. Ref.7
Mutagenesis1821D → A: Abolishes both exoribonuclease and polyadenylation activities. Ref.8

Secondary structure

........................................ 248
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9UXC2 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: EAB2C2C89DD5854C

FASTA24827,578
        10         20         30         40         50         60 
MREMLQVERP KLILDDGKRT DGRKPDELRS IKIELGVLKN ADGSAIFEMG NTKAIAAVYG 

        70         80         90        100        110        120 
PKEMHPRHLS LPDRAVLRVR YHMTPFSTDE RKNPAPSRRE IELSKVIREA LESAVLVELF 

       130        140        150        160        170        180 
PRTAIDVFTE ILQADAGSRL VSLMAASLAL ADAGIPMRDL IAGVAVGKAD GVIILDLNET 

       190        200        210        220        230        240 
EDMWGEADMP IAMMPSLNQV TLFQLNGSMT PDEFRQAFDL AVKGINIIYN LEREALKSKY 


VEFKEEGV 

« Hide

References

« Hide 'large scale' references
[1]"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2."
Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. expand/collapse author list , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W.
Genome 43:116-136(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]"The complete genome of the crenarchaeon Sulfolobus solfataricus P2."
She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. expand/collapse author list , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.
Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]"An exosome-like complex in Sulfolobus solfataricus."
Evguenieva-Hackenberg E., Walter P., Hochleitner E., Lottspeich F., Klug G.
EMBO Rep. 4:889-893(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EXOSOME.
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[4]"Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus."
Walter P., Klein F., Lorentzen E., Ilchmann A., Klug G., Evguenieva-Hackenberg E.
Mol. Microbiol. 62:1076-1089(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EXOSOME.
[5]"The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome."
Roppelt V., Klug G., Evguenieva-Hackenberg E.
FEBS Lett. 584:2931-2936(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[6]"Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus."
Witharana C., Roppelt V., Lochnit G., Klug G., Evguenieva-Hackenberg E.
Biochimie 94:1578-1587(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EXOSOME.
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[7]"Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core."
Lorentzen E., Conti E.
Mol. Cell 20:473-481(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH RRP42, SUBUNIT, MUTAGENESIS OF ARG-98 AND ARG-99.
[8]"The archaeal exosome core is a hexameric ring structure with three catalytic subunits."
Lorentzen E., Walter P., Fribourg S., Evguenieva-Hackenberg E., Klug G., Conti E.
Nat. Struct. Mol. Biol. 12:575-581(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH RRP42, FUNCTION, SUBUNIT, MUTAGENESIS OF ASP-182.
[9]"RNA channelling by the archaeal exosome."
Lorentzen E., Dziembowski A., Lindner D., Seraphin B., Conti E.
EMBO Rep. 8:470-476(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH RRP42 AND RRP4, FUNCTION, RNA-BINDING, SUBUNIT.
[10]"Crystal structure of the S. solfataricus archaeal exosome reveals conformational flexibility in the RNA-binding ring."
Lu C., Ding F., Ke A.
PLoS ONE 5:E8739-E8739(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 4-248 IN COMPLEX WITH RRP42 AND RRP4.
[11]"Crystal structure of a 9-subunit archaeal exosome in pre-catalytic states of the phosphorolytic reaction."
Lorentzen E., Conti E.
Archaea 2012:721869-721869(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF MUTANT ALA-182 IN COMPLEX WITH RRP42 AND RRP4, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y18930 Genomic DNA. Translation: CAB57569.1.
AE006641 Genomic DNA. Translation: AAK41031.1.
PIRH90221.
RefSeqNP_342241.1. NC_002754.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2BR2X-ray2.80B/D/F/H/J/L/N/P/R/T/V/X1-248[»]
2C37X-ray2.80B/D/F/H/J/L/N/P/R/T/V/X1-248[»]
2C38X-ray3.10B/D/F/H/J/L/N/P/R/T/V/X1-248[»]
2C39X-ray3.30B/D/F/H/J/L/N/P/R/T/V/X1-248[»]
2JE6X-ray1.60B1-248[»]
2JEAX-ray2.33B1-248[»]
2JEBX-ray2.40B1-248[»]
3L7ZX-ray2.41B/E/H4-248[»]
4BA1X-ray1.80B1-248[»]
4BA2X-ray2.50B1-248[»]
ProteinModelPortalQ9UXC2.
SMRQ9UXC2. Positions 1-248.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-58154N.
IntActQ9UXC2. 1 interaction.
MINTMINT-7891191.
STRING273057.SSO0735.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK41031; AAK41031; SSO0735.
GeneID1454998.
KEGGsso:SSO0735.

Phylogenomic databases

eggNOGCOG0689.
HOGENOMHOG000229515.
KOK11600.
OMAYRYNMAA.

Enzyme and pathway databases

BioCycSSOL273057:GCH2-696-MONOMER.

Family and domain databases

Gene3D3.30.230.70. 1 hit.
HAMAPMF_00591. Exosome_Rrp41.
InterProIPR011807. ExoRNase_exosome_complex.
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR027408. PNPase/RNase_PH_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PfamPF01138. RNase_PH. 1 hit.
PF03725. RNase_PH_C. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55666. SSF55666. 1 hit.
TIGRFAMsTIGR02065. ECX1. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ9UXC2.

Entry information

Entry nameRRP41_SULSO
AccessionPrimary (citable) accession number: Q9UXC2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references