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Q9UXC2

- RRP41_SULSO

UniProt

Q9UXC2 - RRP41_SULSO

Protein

Exosome complex component Rrp41

Gene

rrp41

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'->5' exoribonuclease activity. Can also synthesize heteropolymeric RNA-tails. Binds RNA.3 PublicationsUniRule annotation

    GO - Molecular functioni

    1. exoribonuclease activity, producing 5'-phosphomonoesters Source: InterPro

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-696-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exosome complex component Rrp41UniRule annotation (EC:3.1.13.-UniRule annotation)
    Gene namesi
    Name:rrp41UniRule annotation
    Ordered Locus Names:SSO0735
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. exosome (RNase complex) Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Exosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi98 – 981R → E: Abolishes exoribonuclease activity; when associated with E-99. 1 Publication
    Mutagenesisi99 – 991R → E: Abolishes exoribonuclease activity; when associated with E-98. 1 Publication
    Mutagenesisi182 – 1821D → A: Abolishes both exoribonuclease and polyadenylation activities. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 248248Exosome complex component Rrp41PRO_0000139991Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.6 PublicationsUniRule annotation

    Protein-protein interaction databases

    DIPiDIP-58154N.
    IntActiQ9UXC2. 1 interaction.
    MINTiMINT-7891191.
    STRINGi273057.SSO0735.

    Structurei

    Secondary structure

    1
    248
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi15 – 173
    Beta strandi31 – 366
    Beta strandi39 – 4911
    Beta strandi52 – 6312
    Helixi67 – 693
    Beta strandi72 – 743
    Beta strandi76 – 838
    Beta strandi87 – 904
    Helixi98 – 11215
    Helixi117 – 1193
    Beta strandi123 – 13311
    Helixi138 – 15215
    Beta strandi157 – 1593
    Beta strandi162 – 1698
    Beta strandi172 – 1765
    Helixi179 – 1846
    Beta strandi185 – 19410
    Helixi195 – 1973
    Beta strandi199 – 2079
    Helixi211 – 23727
    Helixi238 – 2403

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2BR2X-ray2.80B/D/F/H/J/L/N/P/R/T/V/X1-248[»]
    2C37X-ray2.80B/D/F/H/J/L/N/P/R/T/V/X1-248[»]
    2C38X-ray3.10B/D/F/H/J/L/N/P/R/T/V/X1-248[»]
    2C39X-ray3.30B/D/F/H/J/L/N/P/R/T/V/X1-248[»]
    2JE6X-ray1.60B1-248[»]
    2JEAX-ray2.33B1-248[»]
    2JEBX-ray2.40B1-248[»]
    3L7ZX-ray2.41B/E/H4-248[»]
    4BA1X-ray1.80B1-248[»]
    4BA2X-ray2.50B1-248[»]
    ProteinModelPortaliQ9UXC2.
    SMRiQ9UXC2. Positions 1-248.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9UXC2.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNase PH family. Rrp41 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0689.
    HOGENOMiHOG000229515.
    KOiK11600.
    OMAiYRYNMAA.

    Family and domain databases

    Gene3Di3.30.230.70. 1 hit.
    HAMAPiMF_00591. Exosome_Rrp41.
    InterProiIPR011807. ExoRNase_exosome_complex.
    IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR027408. PNPase/RNase_PH_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view]
    PfamiPF01138. RNase_PH. 1 hit.
    PF03725. RNase_PH_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55666. SSF55666. 1 hit.
    TIGRFAMsiTIGR02065. ECX1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9UXC2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREMLQVERP KLILDDGKRT DGRKPDELRS IKIELGVLKN ADGSAIFEMG    50
    NTKAIAAVYG PKEMHPRHLS LPDRAVLRVR YHMTPFSTDE RKNPAPSRRE 100
    IELSKVIREA LESAVLVELF PRTAIDVFTE ILQADAGSRL VSLMAASLAL 150
    ADAGIPMRDL IAGVAVGKAD GVIILDLNET EDMWGEADMP IAMMPSLNQV 200
    TLFQLNGSMT PDEFRQAFDL AVKGINIIYN LEREALKSKY VEFKEEGV 248
    Length:248
    Mass (Da):27,578
    Last modified:May 1, 2000 - v1
    Checksum:iEAB2C2C89DD5854C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y18930 Genomic DNA. Translation: CAB57569.1.
    AE006641 Genomic DNA. Translation: AAK41031.1.
    PIRiH90221.
    RefSeqiNP_342241.1. NC_002754.1.
    WP_010923057.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK41031; AAK41031; SSO0735.
    GeneIDi1454998.
    KEGGisso:SSO0735.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y18930 Genomic DNA. Translation: CAB57569.1 .
    AE006641 Genomic DNA. Translation: AAK41031.1 .
    PIRi H90221.
    RefSeqi NP_342241.1. NC_002754.1.
    WP_010923057.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2BR2 X-ray 2.80 B/D/F/H/J/L/N/P/R/T/V/X 1-248 [» ]
    2C37 X-ray 2.80 B/D/F/H/J/L/N/P/R/T/V/X 1-248 [» ]
    2C38 X-ray 3.10 B/D/F/H/J/L/N/P/R/T/V/X 1-248 [» ]
    2C39 X-ray 3.30 B/D/F/H/J/L/N/P/R/T/V/X 1-248 [» ]
    2JE6 X-ray 1.60 B 1-248 [» ]
    2JEA X-ray 2.33 B 1-248 [» ]
    2JEB X-ray 2.40 B 1-248 [» ]
    3L7Z X-ray 2.41 B/E/H 4-248 [» ]
    4BA1 X-ray 1.80 B 1-248 [» ]
    4BA2 X-ray 2.50 B 1-248 [» ]
    ProteinModelPortali Q9UXC2.
    SMRi Q9UXC2. Positions 1-248.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-58154N.
    IntActi Q9UXC2. 1 interaction.
    MINTi MINT-7891191.
    STRINGi 273057.SSO0735.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK41031 ; AAK41031 ; SSO0735 .
    GeneIDi 1454998.
    KEGGi sso:SSO0735.

    Phylogenomic databases

    eggNOGi COG0689.
    HOGENOMi HOG000229515.
    KOi K11600.
    OMAi YRYNMAA.

    Enzyme and pathway databases

    BioCyci SSOL273057:GCH2-696-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9UXC2.

    Family and domain databases

    Gene3Di 3.30.230.70. 1 hit.
    HAMAPi MF_00591. Exosome_Rrp41.
    InterProi IPR011807. ExoRNase_exosome_complex.
    IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR027408. PNPase/RNase_PH_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view ]
    Pfami PF01138. RNase_PH. 1 hit.
    PF03725. RNase_PH_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55666. SSF55666. 1 hit.
    TIGRFAMsi TIGR02065. ECX1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    3. Cited for: INTERACTION WITH EXOSOME.
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    4. "Characterization of native and reconstituted exosome complexes from the hyperthermophilic archaeon Sulfolobus solfataricus."
      Walter P., Klein F., Lorentzen E., Ilchmann A., Klug G., Evguenieva-Hackenberg E.
      Mol. Microbiol. 62:1076-1089(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EXOSOME.
    5. "The evolutionarily conserved subunits Rrp4 and Csl4 confer different substrate specificities to the archaeal exosome."
      Roppelt V., Klug G., Evguenieva-Hackenberg E.
      FEBS Lett. 584:2931-2936(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    6. "Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus."
      Witharana C., Roppelt V., Lochnit G., Klug G., Evguenieva-Hackenberg E.
      Biochimie 94:1578-1587(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EXOSOME.
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    7. "Structural basis of 3' end RNA recognition and exoribonucleolytic cleavage by an exosome RNase PH core."
      Lorentzen E., Conti E.
      Mol. Cell 20:473-481(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH RRP42, SUBUNIT, MUTAGENESIS OF ARG-98 AND ARG-99.
    8. "The archaeal exosome core is a hexameric ring structure with three catalytic subunits."
      Lorentzen E., Walter P., Fribourg S., Evguenieva-Hackenberg E., Klug G., Conti E.
      Nat. Struct. Mol. Biol. 12:575-581(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH RRP42, FUNCTION, SUBUNIT, MUTAGENESIS OF ASP-182.
    9. Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEX WITH RRP42 AND RRP4, FUNCTION, RNA-BINDING, SUBUNIT.
    10. "Crystal structure of the S. solfataricus archaeal exosome reveals conformational flexibility in the RNA-binding ring."
      Lu C., Ding F., Ke A.
      PLoS ONE 5:E8739-E8739(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.41 ANGSTROMS) OF 4-248 IN COMPLEX WITH RRP42 AND RRP4.
    11. "Crystal structure of a 9-subunit archaeal exosome in pre-catalytic states of the phosphorolytic reaction."
      Lorentzen E., Conti E.
      Archaea 2012:721869-721869(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF MUTANT ALA-182 IN COMPLEX WITH RRP42 AND RRP4, SUBUNIT.

    Entry informationi

    Entry nameiRRP41_SULSO
    AccessioniPrimary (citable) accession number: Q9UXC2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 25, 2003
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3