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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity).By similarity

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei537 – 5371ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-684-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SSO0722
ORF Names:C10_008
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 986986Isoleucine--tRNA ligasePRO_0000098593Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi273057.SSO0722.

Structurei

3D structure databases

ProteinModelPortaliQ9UXB1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi534 – 5385"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
InParanoidiQ9UXB1.
KOiK01870.
OMAiVLGDWDN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UXB1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKDCILRYQ RVLGKRVHDQ PGYDTHGLPI EVATEKLLGI SNKQEIIDKI
60 70 80 90 100
GVETFINKCK EFALSNADKM TQNFKNVGVF MDWERPYYTL DPSYISSSWS
110 120 130 140 150
VIKKAYEKGM LDKGTAVLHW CPRCETTLSD YEVSEYRDLE DPSIYVKFKI
160 170 180 190 200
KGEKNRYLLI WTTTPWTIPS NVFVMINKDY DYADVEVNGE ILVIAKDRVE
210 220 230 240 250
AVMKEASITN YKILRTYKGS ELIGIKYEHP LREFVSAQTK LDDFHQVVDA
260 270 280 290 300
GNIVTLTDGT GLVHSATGHG EEDFTVGQKY GFPVVMFVND RGEFTEEGGK
310 320 330 340 350
YKGLKVRDAS KAIISDLKSK NTLFFEGKIV HRYPVCWRCK TPLILRAIDQ
360 370 380 390 400
WFIRVTKIKD KMLNEIEKVN WIPDWGKSRI SNMVKELRDW VISRQRFWGT
410 420 430 440 450
PLPIWICERC NNVMVVGSKE ELESIAIDPV PNDLHRPWID NVRVKCNKCG
460 470 480 490 500
GVAKRIPDVA DVWFDSGVAF FASLGKDWQE KWKELGPVDL VLEGHDQLRG
510 520 530 540 550
WFFSLLRSGL ILLDRAPYTS VLVHGFMLDE QGREMHKSLG NYVEPSVVVE
560 570 580 590 600
KYGRDILRLW LLRNTTWEDA KFSWKALELT KRDLQIIWNT FVFASMYMNL
610 620 630 640 650
DNFEPDKYTL DDIIKYAKIE DLWILSRFNS MLKKVNESMK DYKVHEMTNY
660 670 680 690 700
LINFLIEDVS RFYIRLIRKR AWIEANTQDK IAMYYILYYI LKQWIILAST
710 720 730 740 750
IIPFISEKIY KSFVVNAKES VSMESSINYD ERFIDNELER AFEVAREINE
760 770 780 790 800
ASLNARAKAG IKLRWPLAKV YIFIENEDTL AKVGRIKDVL ISMLNAKDIE
810 820 830 840 850
ISKIEGFKSF SKYKVEPNRS IIGKEYKSMS PKIVEYIENN RDIIAMDILN
860 870 880 890 900
KKQHVAKIDN FDIILNASYV IISEETVEGF ISSKFSKGIV VISKEISESE
910 920 930 940 950
EEEGLIRDII RRIQFMRKQL KLNVLDYIEI SMKVPEERVK TIQKWEEFIK
960 970 980
SETRASNIIL GEAKGDITMD WDIEGESYII GIKKST
Length:986
Mass (Da):114,714
Last modified:May 1, 2000 - v1
Checksum:i0177A3C31280F5A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18930 Genomic DNA. Translation: CAB57581.1.
AE006641 Genomic DNA. Translation: AAK41020.1.
PIRiE90220.
RefSeqiNP_342230.1. NC_002754.1.
WP_010923054.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41020; AAK41020; SSO0722.
GeneIDi1454987.
KEGGisso:SSO0722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18930 Genomic DNA. Translation: CAB57581.1.
AE006641 Genomic DNA. Translation: AAK41020.1.
PIRiE90220.
RefSeqiNP_342230.1. NC_002754.1.
WP_010923054.1. NC_002754.1.

3D structure databases

ProteinModelPortaliQ9UXB1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO0722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK41020; AAK41020; SSO0722.
GeneIDi1454987.
KEGGisso:SSO0722.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
InParanoidiQ9UXB1.
KOiK01870.
OMAiVLGDWDN.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-684-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Entry informationi

Entry nameiSYI_SULSO
AccessioniPrimary (citable) accession number: Q9UXB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.