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Q9UXB1

- SYI_SULSO

UniProt

Q9UXB1 - SYI_SULSO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.By similarity

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Cofactori

    Zinc.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei537 – 5371ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-684-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:ileS
    Ordered Locus Names:SSO0722
    ORF Names:C10_008
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 986986Isoleucine--tRNA ligasePRO_0000098593Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi273057.SSO0722.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UXB1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi534 – 5385"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRYVEGKW.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9UXB1-1 [UniParc]FASTAAdd to Basket

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    MIKDCILRYQ RVLGKRVHDQ PGYDTHGLPI EVATEKLLGI SNKQEIIDKI    50
    GVETFINKCK EFALSNADKM TQNFKNVGVF MDWERPYYTL DPSYISSSWS 100
    VIKKAYEKGM LDKGTAVLHW CPRCETTLSD YEVSEYRDLE DPSIYVKFKI 150
    KGEKNRYLLI WTTTPWTIPS NVFVMINKDY DYADVEVNGE ILVIAKDRVE 200
    AVMKEASITN YKILRTYKGS ELIGIKYEHP LREFVSAQTK LDDFHQVVDA 250
    GNIVTLTDGT GLVHSATGHG EEDFTVGQKY GFPVVMFVND RGEFTEEGGK 300
    YKGLKVRDAS KAIISDLKSK NTLFFEGKIV HRYPVCWRCK TPLILRAIDQ 350
    WFIRVTKIKD KMLNEIEKVN WIPDWGKSRI SNMVKELRDW VISRQRFWGT 400
    PLPIWICERC NNVMVVGSKE ELESIAIDPV PNDLHRPWID NVRVKCNKCG 450
    GVAKRIPDVA DVWFDSGVAF FASLGKDWQE KWKELGPVDL VLEGHDQLRG 500
    WFFSLLRSGL ILLDRAPYTS VLVHGFMLDE QGREMHKSLG NYVEPSVVVE 550
    KYGRDILRLW LLRNTTWEDA KFSWKALELT KRDLQIIWNT FVFASMYMNL 600
    DNFEPDKYTL DDIIKYAKIE DLWILSRFNS MLKKVNESMK DYKVHEMTNY 650
    LINFLIEDVS RFYIRLIRKR AWIEANTQDK IAMYYILYYI LKQWIILAST 700
    IIPFISEKIY KSFVVNAKES VSMESSINYD ERFIDNELER AFEVAREINE 750
    ASLNARAKAG IKLRWPLAKV YIFIENEDTL AKVGRIKDVL ISMLNAKDIE 800
    ISKIEGFKSF SKYKVEPNRS IIGKEYKSMS PKIVEYIENN RDIIAMDILN 850
    KKQHVAKIDN FDIILNASYV IISEETVEGF ISSKFSKGIV VISKEISESE 900
    EEEGLIRDII RRIQFMRKQL KLNVLDYIEI SMKVPEERVK TIQKWEEFIK 950
    SETRASNIIL GEAKGDITMD WDIEGESYII GIKKST 986
    Length:986
    Mass (Da):114,714
    Last modified:May 1, 2000 - v1
    Checksum:i0177A3C31280F5A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y18930 Genomic DNA. Translation: CAB57581.1.
    AE006641 Genomic DNA. Translation: AAK41020.1.
    PIRiE90220.
    RefSeqiNP_342230.1. NC_002754.1.
    WP_010923054.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK41020; AAK41020; SSO0722.
    GeneIDi1454987.
    KEGGisso:SSO0722.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y18930 Genomic DNA. Translation: CAB57581.1 .
    AE006641 Genomic DNA. Translation: AAK41020.1 .
    PIRi E90220.
    RefSeqi NP_342230.1. NC_002754.1.
    WP_010923054.1. NC_002754.1.

    3D structure databases

    ProteinModelPortali Q9UXB1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 273057.SSO0722.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK41020 ; AAK41020 ; SSO0722 .
    GeneIDi 1454987.
    KEGGi sso:SSO0722.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RYVEGKW.

    Enzyme and pathway databases

    BioCyci SSOL273057:GCH2-684-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

    Entry informationi

    Entry nameiSYI_SULSO
    AccessioniPrimary (citable) accession number: Q9UXB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3