Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9UXB1 (SYI_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:SSO0722
ORF Names:C10_008
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length986 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Cofactor

Zinc By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 986986Isoleucine--tRNA ligase
PRO_0000098593

Regions

Motif534 – 5385"KMSKS" region

Sites

Binding site5371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9UXB1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 0177A3C31280F5A1

FASTA986114,714
        10         20         30         40         50         60 
MIKDCILRYQ RVLGKRVHDQ PGYDTHGLPI EVATEKLLGI SNKQEIIDKI GVETFINKCK 

        70         80         90        100        110        120 
EFALSNADKM TQNFKNVGVF MDWERPYYTL DPSYISSSWS VIKKAYEKGM LDKGTAVLHW 

       130        140        150        160        170        180 
CPRCETTLSD YEVSEYRDLE DPSIYVKFKI KGEKNRYLLI WTTTPWTIPS NVFVMINKDY 

       190        200        210        220        230        240 
DYADVEVNGE ILVIAKDRVE AVMKEASITN YKILRTYKGS ELIGIKYEHP LREFVSAQTK 

       250        260        270        280        290        300 
LDDFHQVVDA GNIVTLTDGT GLVHSATGHG EEDFTVGQKY GFPVVMFVND RGEFTEEGGK 

       310        320        330        340        350        360 
YKGLKVRDAS KAIISDLKSK NTLFFEGKIV HRYPVCWRCK TPLILRAIDQ WFIRVTKIKD 

       370        380        390        400        410        420 
KMLNEIEKVN WIPDWGKSRI SNMVKELRDW VISRQRFWGT PLPIWICERC NNVMVVGSKE 

       430        440        450        460        470        480 
ELESIAIDPV PNDLHRPWID NVRVKCNKCG GVAKRIPDVA DVWFDSGVAF FASLGKDWQE 

       490        500        510        520        530        540 
KWKELGPVDL VLEGHDQLRG WFFSLLRSGL ILLDRAPYTS VLVHGFMLDE QGREMHKSLG 

       550        560        570        580        590        600 
NYVEPSVVVE KYGRDILRLW LLRNTTWEDA KFSWKALELT KRDLQIIWNT FVFASMYMNL 

       610        620        630        640        650        660 
DNFEPDKYTL DDIIKYAKIE DLWILSRFNS MLKKVNESMK DYKVHEMTNY LINFLIEDVS 

       670        680        690        700        710        720 
RFYIRLIRKR AWIEANTQDK IAMYYILYYI LKQWIILAST IIPFISEKIY KSFVVNAKES 

       730        740        750        760        770        780 
VSMESSINYD ERFIDNELER AFEVAREINE ASLNARAKAG IKLRWPLAKV YIFIENEDTL 

       790        800        810        820        830        840 
AKVGRIKDVL ISMLNAKDIE ISKIEGFKSF SKYKVEPNRS IIGKEYKSMS PKIVEYIENN 

       850        860        870        880        890        900 
RDIIAMDILN KKQHVAKIDN FDIILNASYV IISEETVEGF ISSKFSKGIV VISKEISESE 

       910        920        930        940        950        960 
EEEGLIRDII RRIQFMRKQL KLNVLDYIEI SMKVPEERVK TIQKWEEFIK SETRASNIIL 

       970        980 
GEAKGDITMD WDIEGESYII GIKKST 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y18930 Genomic DNA. Translation: CAB57581.1.
AE006641 Genomic DNA. Translation: AAK41020.1.
PIRE90220.
RefSeqNP_342230.1. NC_002754.1.

3D structure databases

ProteinModelPortalQ9UXB1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273057.SSO0722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK41020; AAK41020; SSO0722.
GeneID1454987.
KEGGsso:SSO0722.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246403.
KOK01870.
OMARYVEGKW.

Enzyme and pathway databases

BioCycSSOL273057:GCH2-684-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYI_SULSO
AccessionPrimary (citable) accession number: Q9UXB1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries