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Q9UXB1

- SYI_SULSO

UniProt

Q9UXB1 - SYI_SULSO

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Protein
Isoleucine--tRNA ligase
Gene
ileS, SSO0722, C10_008
Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Cofactori

Zinc By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei537 – 5371ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-684-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SSO0722
ORF Names:C10_008
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 986986Isoleucine--tRNA ligase
PRO_0000098593Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi273057.SSO0722.

Structurei

3D structure databases

ProteinModelPortaliQ9UXB1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi534 – 5385"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRYVEGKW.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UXB1-1 [UniParc]FASTAAdd to Basket

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MIKDCILRYQ RVLGKRVHDQ PGYDTHGLPI EVATEKLLGI SNKQEIIDKI    50
GVETFINKCK EFALSNADKM TQNFKNVGVF MDWERPYYTL DPSYISSSWS 100
VIKKAYEKGM LDKGTAVLHW CPRCETTLSD YEVSEYRDLE DPSIYVKFKI 150
KGEKNRYLLI WTTTPWTIPS NVFVMINKDY DYADVEVNGE ILVIAKDRVE 200
AVMKEASITN YKILRTYKGS ELIGIKYEHP LREFVSAQTK LDDFHQVVDA 250
GNIVTLTDGT GLVHSATGHG EEDFTVGQKY GFPVVMFVND RGEFTEEGGK 300
YKGLKVRDAS KAIISDLKSK NTLFFEGKIV HRYPVCWRCK TPLILRAIDQ 350
WFIRVTKIKD KMLNEIEKVN WIPDWGKSRI SNMVKELRDW VISRQRFWGT 400
PLPIWICERC NNVMVVGSKE ELESIAIDPV PNDLHRPWID NVRVKCNKCG 450
GVAKRIPDVA DVWFDSGVAF FASLGKDWQE KWKELGPVDL VLEGHDQLRG 500
WFFSLLRSGL ILLDRAPYTS VLVHGFMLDE QGREMHKSLG NYVEPSVVVE 550
KYGRDILRLW LLRNTTWEDA KFSWKALELT KRDLQIIWNT FVFASMYMNL 600
DNFEPDKYTL DDIIKYAKIE DLWILSRFNS MLKKVNESMK DYKVHEMTNY 650
LINFLIEDVS RFYIRLIRKR AWIEANTQDK IAMYYILYYI LKQWIILAST 700
IIPFISEKIY KSFVVNAKES VSMESSINYD ERFIDNELER AFEVAREINE 750
ASLNARAKAG IKLRWPLAKV YIFIENEDTL AKVGRIKDVL ISMLNAKDIE 800
ISKIEGFKSF SKYKVEPNRS IIGKEYKSMS PKIVEYIENN RDIIAMDILN 850
KKQHVAKIDN FDIILNASYV IISEETVEGF ISSKFSKGIV VISKEISESE 900
EEEGLIRDII RRIQFMRKQL KLNVLDYIEI SMKVPEERVK TIQKWEEFIK 950
SETRASNIIL GEAKGDITMD WDIEGESYII GIKKST 986
Length:986
Mass (Da):114,714
Last modified:May 1, 2000 - v1
Checksum:i0177A3C31280F5A1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y18930 Genomic DNA. Translation: CAB57581.1.
AE006641 Genomic DNA. Translation: AAK41020.1.
PIRiE90220.
RefSeqiNP_342230.1. NC_002754.1.
WP_010923054.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK41020; AAK41020; SSO0722.
GeneIDi1454987.
KEGGisso:SSO0722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y18930 Genomic DNA. Translation: CAB57581.1 .
AE006641 Genomic DNA. Translation: AAK41020.1 .
PIRi E90220.
RefSeqi NP_342230.1. NC_002754.1.
WP_010923054.1. NC_002754.1.

3D structure databases

ProteinModelPortali Q9UXB1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 273057.SSO0722.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK41020 ; AAK41020 ; SSO0722 .
GeneIDi 1454987.
KEGGi sso:SSO0722.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RYVEGKW.

Enzyme and pathway databases

BioCyci SSOL273057:GCH2-684-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Entry informationi

Entry nameiSYI_SULSO
AccessioniPrimary (citable) accession number: Q9UXB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi