Q9UX21 (PURQ_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 1
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase I
Short name=FGAM synthase I

Gene names

Name:	purQ
Ordered Locus Names:	SSO0628
ORF Names:	C08_022

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00421
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00421
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00421.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Phosphoribosylformylglycinamidine synthase 1 HAMAP MF_00421		PRO_0000100619
Regions
	Domain	1 – 224	224	Glutamine amidotransferase type-1
Sites
	Active site	84	1	Nucleophile By similarity
	Active site	196	1	By similarity
	Active site	198	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UX21 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8D8C25A34ED62D76	FASTA	224	24,686
	10 20 30 40 50 60 MIAIIKFPGT TCETDVYKAL IEAGVPTVIV KYKDFDPDRY NGVILPGGFS FGDYLRAGSI 70 80 90 100 110 120 AASTETMKKV KQMAEDGKIV IGICNGFQIL VESGLLKGAL LPNLKLRFIS KWVYLKVIRA 130 140 150 160 170 180 DTILTKGLDK KIIRMPIAHA EGRYYVDDID YAKTHMVLQY CDENGNISED VNPNGSLLNI 190 200 210 220 ASIANEEGNV IGMMPHPERA SFKLTSIDGT VDGLILLRRL GEWA

References

[1]

"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2." Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W. Genome 43:116-136(2000) [PubMed: 10701121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

[2]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18930 Genomic DNA. Translation: CAB57673.1. AE006641 Genomic DNA. Translation: AAK40939.1.
PIR	D90210.
RefSeq	NP_342149.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q9UX21.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1454906.
GenomeReviews	Gene locus SSO0628 in contig AE006641_GR.
KEGG	sso:SSO0628.
NMPDR	fig|273057.1.peg.572.
Phylogenomic databases
HOGENOM	HBG302712.
OMA	FPGTNCD.
ProtClustDB	PRK03619.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0628-MONOMER.
Family and domain databases
HAMAP	MF_00421. PurQ. [Tree]
InterPro	IPR017926. GATASE_1. IPR010075. PRibForGlyAmidine_synth_I. IPR002818. ThiJ/PfpI. [Graphical view]
KO	K01952.
Pfam	PF01965. DJ-1_PfpI. 1 hit. [Graphical view]
PIRSF	PIRSF001586. FGAM_synth_I. 1 hit.
TIGRFAMs	TIGR01737. FGAM_synth_I. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURQ_SULSO

Accession

Primary (citable) accession number: Q9UX21

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program