Q9UX10 (PYRF_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotidine 5'-phosphate decarboxylase
EC=4.1.1.23

Alternative name(s):
OMP decarboxylase
Short name=OMPDCase
Short name=OMPdecase

Gene names

Name:	pyrF
Ordered Locus Names:	SSO0616
ORF Names:	C08_034

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) By similarity. HAMAP MF_01200_A
Catalytic activity	Orotidine 5'-phosphate = UMP + CO2. HAMAP MF_01200_A
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2. HAMAP MF_01200_A
Subunit structure	Homodimer By similarity. HAMAP MF_01200_A
Sequence similarities	Belongs to the OMP decarboxylase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: InterPro 'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Orotidine 5'-phosphate decarboxylase HAMAP MF_01200_A		PRO_0000134618
Regions
	Region	59 – 68	10	Substrate binding By similarity
	Region	164 – 174	11	Substrate binding By similarity
Sites
	Active site	61	1	Proton donor By similarity
	Binding site	11	1	Substrate By similarity
	Binding site	30	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	187	1	Substrate; via amide nitrogen By similarity
	Binding site	188	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UX10 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 48F3E83DB0F043FF	FASTA	222	24,701
	10 20 30 40 50 60 MLKSRVILAM DKPLSYQVLK EMENELYGIK VGLPLVLDLG VDKTRELLIG LDVEEIIVDF 70 80 90 100 110 120 KLADIGYIMK SIVERLSFAN SFIAHSFIGV KGSLDELKRY LDANSKNLYL VAVMSHEGWS 130 140 150 160 170 180 TLFADYIKNV IREISPKGIV VGGTKLDHIT QYRRDFEKMT IVSPGMGSQG GSYGDAVCAG 190 200 210 220 ADYEIIGRSI YNAGNPLTAL RTINKIIEDK VMKCKGAIFR KK

References

[1]	"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2." Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W. Genome 43:116-136(2000) [PubMed: 10701121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18930 Genomic DNA. Translation: CAB57684.1. AE006641 Genomic DNA. Translation: AAK40927.1.
PIR	H90208.
RefSeq	NP_342137.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q9UX10.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1454894.
GenomeReviews	Gene locus SSO0616 in contig AE006641_GR.
KEGG	sso:SSO0616.
NMPDR	fig|273057.1.peg.560.
Phylogenomic databases
HOGENOM	HBG625253.
OMA	TEMSHPG.
ProtClustDB	CLSK802793.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0616-MONOMER.
Family and domain databases
HAMAP	MF_01200_A. OMPdecase_type1_A. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase. IPR001754. OMPdeCOase_dom. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01591.
Pfam	PF00215. OMPdecase. 1 hit. [Graphical view]
SMART	SM00934. OMPdecase. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
PROSITE	PS00156. OMPDECASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRF_SULSO

Accession

Primary (citable) accession number: Q9UX10

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program