Q9UX09 (PYRE_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	SSO0615
ORF Names:	C08_035

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110790
Regions
	Region	111 – 119	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	86	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	90	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	92	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	115	1	Orotate By similarity
	Binding site	143	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UX09 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E4CF07445B7FEBF4	FASTA	195	21,614
	10 20 30 40 50 60 MNFAEVLLER KLLLIGSFVL TSGKVSPYYL DLRPLPNYPE FYDIVNQAIK KAKDIPHDII 70 80 90 100 110 120 VGIATGGVPL SAFIACNLKE PMGYIRIEKK GHGTNRTLEL DVKGKRVLLV DDVATTGVSI 130 140 150 160 170 180 EKATLEILNG GGKVSDALVI IDRQEGASQR LEKLGVKLHS LFKISEILDE LLKSDKLKDN 190 EKKSILDYLV KNVEK

References

[1]

"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2." Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W. Genome 43:116-136(2000) [PubMed: 10701121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

[2]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18930 Genomic DNA. Translation: CAB57685.1. AE006641 Genomic DNA. Translation: AAK40926.1.
PIR	G90208.
RefSeq	NP_342136.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q9UX09.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1454893.
GenomeReviews	Gene locus SSO0615 in contig AE006641_GR.
KEGG	sso:SSO0615.
NMPDR	fig|273057.1.peg.559.
Phylogenomic databases
HOGENOM	HBG404341.
OMA	LSYPRIR.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0615-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_SULSO

Accession

Primary (citable) accession number: Q9UX09

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program