Q9UX05 (PYRC_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	SSO0611
ORF Names:	C08_039

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220_A
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00220_A
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220_A
Subunit structure	Homodimer By similarity. HAMAP MF_00220_A
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Dihydroorotase HAMAP MF_00220_A		PRO_0000147280
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	160	1	Zinc 2 By similarity
	Metal binding	197	1	Zinc 2 By similarity
	Metal binding	259	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UX05 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F66C94D6988E41E7	FASTA	390	44,333
	10 20 30 40 50 60 MMILWIMGKA YLSKEIRDVC INFDRRIKEI KSICKPDIAL PKGTLILPGA IDLHTHIRGL 70 80 90 100 110 120 KLAYKEDVVS GTSEASYGGI TLIGDMPNSV PFVNTMETIT AKLREFEYYS RVDYFVYSGV 130 140 150 160 170 180 TKDLKKVDKF PIAGYKIFPE DLEKEETLEV LKSMKLKILH PEVPLALRGN RKLRLNIWYE 190 200 210 220 230 240 IGALYYVKGY QNVHITHATN IRTVRLAKEL GFTVDITPHH LLVDREKECL TKVNPPIRDT 250 260 270 280 290 300 NERLWLLQAI NEVDTVVSDH APHASFEKQQ PYEICPPGIA ALSFTVPFIL TLVSKGIISI 310 320 330 340 350 360 DRAVELISTN PARILNIPYG EIKENNYANF TIIQFKDWRY STKYSKVIET PLDGFPLRAS 370 380 390 IYMTIIQGKV GSLEGEVFPV KGINPFGENK

References

[1]

"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2." Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W. Genome 43:116-136(2000) [PubMed: 10701121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

[2]

"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18930 Genomic DNA. Translation: CAB57689.1. AE006641 Genomic DNA. Translation: AAK40922.1.
PIR	C90208.
RefSeq	NP_342132.1. NC_002754.1.
3D structure databases
ProteinModelPortal	Q9UX05.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1454889.
GenomeReviews	Gene locus SSO0611 in contig AE006641_GR.
KEGG	sso:SSO0611.
NMPDR	fig|273057.1.peg.555.
Phylogenomic databases
HOGENOM	HBG724623.
OMA	NMLVDDP.
ProtClustDB	PRK00369.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0611-MONOMER.
Family and domain databases
HAMAP	MF_00220_A. PyrC_type2_A. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOase. IPR002195. Dihydroorotase_CS. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PROSITE	PS00482. DIHYDROOROTASE_1. False negative. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_SULSO

Accession

Primary (citable) accession number: Q9UX05

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program