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Q9UX04 (PYRDB_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit
Short name=DHOD B
Short name=DHODase B
Short name=DHOdehase B
EC=1.3.1.14
Alternative name(s):
Dihydrdoorotate oxidase B
Orotate reductase (NADH)
Gene names
Name:pyrD
Ordered Locus Names:SSO0610
ORF Names:C08_040
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with NAD+ as electron acceptor By similarity. HAMAP MF_00224

Catalytic activity

(S)-dihydroorotate + NAD+ = orotate + NADH. HAMAP MF_00224

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP MF_00224

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1. HAMAP MF_00224

Subunit structure

Heterotetramer of 2 PyrK and 2 PyrD type B subunits By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_00224.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Cellular componentCytoplasm
   LigandFMN
Flavoprotein
NAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' pyrimidine base biosynthetic process

Inferred from electronic annotation. Source: InterPro

UMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydroorotate oxidase activity

Inferred from electronic annotation. Source: InterPro

orotate reductase (NADH) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit HAMAP MF_00224
PRO_0000148417

Regions

Nucleotide binding42 – 432FMN By similarity
Nucleotide binding229 – 2302FMN By similarity
Nucleotide binding251 – 2522FMN By similarity
Region67 – 715Substrate binding By similarity
Region178 – 1792Substrate binding By similarity

Sites

Active site1201Nucleophile
Binding site171FMN By similarity
Binding site421Substrate By similarity
Binding site1171FMN By similarity
Binding site1171Substrate By similarity
Binding site1521FMN By similarity
Binding site1771FMN; via carbonyl oxygen By similarity
Binding site2031FMN; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9UX04 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 65AA1512509A78CC

FASTA29031,154
        10         20         30         40         50         60 
MITIKNITLN DPLIIASGII PDVPNYVETI CEKYKPSAIT TKTVTLNPLE PHKPPTVIKL 

        70         80         90        100        110        120 
HDGIYMNAIG LGNPGAKAIN EIGVLCPLIV SVGGSSINEI KEVVEVVQSK AKIIEINVSS 

       130        140        150        160        170        180 
PNRKGYGESL SKLIGDIIES VKSVTKLPVF VKLGPWDNVI ELAGKALEKG ADGLTLINTI 

       190        200        210        220        230        240 
KGLIIDVETF KPILYYGTGG VSGRCLYPIA LRIIKDVYEE YGVDIIGVGG VYDWTDVIGM 

       250        260        270        280        290 
LAAGAKLVGL GTVLIEKGFS VIEEIRKGLQ SYLLEKGLKF EDIIGISVRK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y18930 Genomic DNA. Translation: CAB57690.1.
AE006641 Genomic DNA. Translation: AAK40921.1.
PIRB90208.
RefSeqNP_342131.1. NC_002754.1.

3D structure databases

ProteinModelPortalQ9UX04.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1454888.
GenomeReviewsGene locus SSO0610 in contig AE006641_GR.
KEGGsso:SSO0610.
NMPDRfig|273057.1.peg.554.

Phylogenomic databases

HOGENOMHBG472415.
OMAHAKGYGM.
ProtClustDBCLSK802790.

Enzyme and pathway databases

BioCycSSOL273057:SSO0610-MONOMER.

Family and domain databases

HAMAPMF_00224. DHO_dh_type1.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR005720. Dihydroorotate_DH.
IPR024920. Dihydroorotate_DH_1.
IPR012135. Dihydroorotate_DH_1_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK00226.
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01037. PyrD_sub1_fam. 1 hit.
PROSITEPS00911. DHODEHASE_1. False negative.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRDB_SULSO
AccessionPrimary (citable) accession number: Q9UX04
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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