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Q9UWY6 (DGGGP_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Digeranylgeranylglyceryl phosphate synthase

Short name=DGGGP synthase
Short name=DGGGPS
EC=2.5.1.42
Alternative name(s):
(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase
Geranylgeranylglycerol-phosphate geranylgeranyltransferase
Gene names
Name:ubiA-2
Ordered Locus Names:SSO0583
ORF Names:C21_016
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length282 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids. Can not use other prenyl donors, i.e. farnesyl diphosphate (FPP) and phytyl diphosphate. Moreover, 4-hydroxybenzoate, 1,4-dihydroxy 2-naphthoate, homogentisate, and alpha-glycerophosphate do not function as prenyl acceptor substrates. Ref.3

Catalytic activity

Geranylgeranyl diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate = diphosphate + 2,3-bis-O-(geranylgeranyl)glycerol 1-phosphate. Ref.3

Cofactor

Magnesium. Can also use Ca2+, but less efficiently. Ref.3

Enzyme regulation

Inhibited by EDTA in vitro. Ref.3

Pathway

Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP-Rule MF_01286

Subcellular location

Cell membrane; Multi-pass membrane protein Probable Ref.3.

Sequence similarities

Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 6.0. HAMAP-Rule MF_01286

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 282282Digeranylgeranylglyceryl phosphate synthase HAMAP-Rule MF_01286
PRO_0000350691

Regions

Transmembrane15 – 3521Helical; Potential
Transmembrane36 – 5621Helical; Potential
Transmembrane81 – 10020Helical; Potential
Transmembrane104 – 12118Helical; Potential
Transmembrane131 – 15121Helical; Potential
Transmembrane159 – 17921Helical; Potential
Transmembrane201 – 22121Helical; Potential
Transmembrane222 – 24221Helical; Potential
Transmembrane260 – 28021Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q9UWY6 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 0E98AFDD6590B052

FASTA28230,915
        10         20         30         40         50         60 
MSLKSYMQLV RIHNVIGAAL GAIMGFLVSS QWYLELKGIL LSALVVGLIA AGGYVINDVY 

        70         80         90        100        110        120 
DVEIDKINKP YRPIPSGKIS VNKAKALSIA LFIIGIALSI LLNIYALVIA LVTAIGLIYY 

       130        140        150        160        170        180 
AKDLKKTGFY GNLLVATTTA LSIFYGGLAF FSDNWLLRII IPTLYAFFLT LIREIVKGIE 

       190        200        210        220        230        240 
DYNGDSLNNV KTLATTLGIN KSWRIAKILL VLLLIISPLP FFIGFNLIYL ILLILVFIPF 

       250        260        270        280 
TILSIIQKET IEGASKARTY LKISAISGII AFLLGSLPFF KG 

« Hide

References

« Hide 'large scale' references
[1]"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2."
Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. expand/collapse author list , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W.
Genome 43:116-136(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]"The complete genome of the crenarchaeon Sulfolobus solfataricus P2."
She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. expand/collapse author list , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.
Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]"(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus. Molecular cloning and characterization of a membrane-intrinsic prenyltransferase involved in the biosynthesis of archaeal ether-linked membrane lipids."
Hemmi H., Shibuya K., Takahashi Y., Nakayama T., Nishino T.
J. Biol. Chem. 279:50197-50203(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, COFACTOR, PH DEPENDENCE, ENZYME REGULATION, SUBCELLULAR LOCATION.
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y18930 Genomic DNA. Translation: CAB57717.1.
AE006641 Genomic DNA. Translation: AAK40896.1.
PIRA99205.
RefSeqNP_342106.1. NC_002754.1.

3D structure databases

ProteinModelPortalQ9UWY6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING273057.SSO0583.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK40896; AAK40896; SSO0583.
GeneID1454863.
KEGGsso:SSO0583.

Phylogenomic databases

eggNOGCOG0382.
HOGENOMHOG000152645.
KOK17105.
OMARTYLELM.
ProtClustDBPRK09573.

Enzyme and pathway databases

BioCycSSOL273057:GCH2-558-MONOMER.
UniPathwayUPA00940.

Family and domain databases

HAMAPMF_01286. DGGGP_synth.
InterProIPR023547. DGGGP_synth.
IPR000537. UbiA_prenyltransferase.
[Graphical view]
PfamPF01040. UbiA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDGGGP_SULSO
AccessionPrimary (citable) accession number: Q9UWY6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: May 1, 2000
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways