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Q9UWW1 (PRIL_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA primase large subunit PriL

EC=2.7.7.-
Gene names
Name:priL
Synonyms:priB
Ordered Locus Names:SSO0557
ORF Names:C21_042
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Can also use dNTPs to synthesize DNA. In addition, possesses a template-independent 3'-terminal nucleotidyl transferase activity. Ref.3

Cofactor

Binds 1 4Fe-4S cluster. Ref.4

Subunit structure

Heterodimer of a small subunit (PriS) and a large subunit (PriL). Ref.3 Ref.5

Sequence similarities

Belongs to the eukaryotic-type primase large subunit family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ORF-c40_009Q9UXG03EBI-8081454,EBI-8081318From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307DNA primase large subunit PriL HAMAP-Rule MF_00701
PRO_0000046787

Sites

Metal binding2161Iron-sulfur (4Fe-4S) By similarity
Metal binding2801Iron-sulfur (4Fe-4S) By similarity
Metal binding2891Iron-sulfur (4Fe-4S) By similarity
Metal binding2931Iron-sulfur (4Fe-4S) By similarity

Secondary structure

................................... 307
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9UWW1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5EF09A913B4ABAC3

FASTA30735,734
        10         20         30         40         50         60 
MALDVKKYPF IKSLDDELKK YGGGITLTDL LLNSTTLIDQ AKDRIQKTKS GDELPHYVSY 

        70         80         90        100        110        120 
NEPVLVFYTT LLSLAILNDV KLIRRYAYAE AKQFRSLLHT ENEENLLEIS KLLDLKINRC 

       130        140        150        160        170        180 
DPIKFYLEKK RRIIQKEFCV HFIDYLKYTK DLKEDWKLSG QILHKGYVYL DKNQLIGLIA 

       190        200        210        220        230        240 
ESIKSKIVEM IRPLNLKEIP EKLKSLIERR GIIPPCIENI LAKEKLNEEE IRTLITFYID 

       250        260        270        280        290        300 
IGKGLSGIVS IMKKYNVSNV EDLYRKYRGD KGTRYIVYSC AKMKQLGLCV SSCNVKNPLQ 


LYFLSNE 

« Hide

References

« Hide 'large scale' references
[1]"Gene content and organization of a 281-kbp contig from the genome of the extremely thermophilic archaeon, Sulfolobus solfataricus P2."
Charlebois R.L., Singh R.K., Chan-Weiher C.C.-Y., Allard G., Chow C., Confalonieri F., Curtis B., Duguet M., Erauso G., Faguy D., Gaasterland T., Garrett R.A., Gordon P., Jeffries A.C., Kozera C., Kushwaha N., Lafleur E., Medina N. expand/collapse author list , Peng X., Penny S.L., She Q., St Jean A., van der Oost J., Young F., Zivanovic Y., Doolittle W.F., Ragan M.A., Sensen C.W.
Genome 43:116-136(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]"The complete genome of the crenarchaeon Sulfolobus solfataricus P2."
She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. expand/collapse author list , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.
Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]"The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities."
Lao-Sirieix S.H., Bell S.D.
J. Mol. Biol. 344:1251-1263(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, GENE NAME.
[4]"An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis."
Klinge S., Hirst J., Maman J.D., Krude T., Pellegrini L.
Nat. Struct. Mol. Biol. 14:875-877(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IRON-SULFUR-BINDING, COFACTOR.
[5]"Structure of the heterodimeric core primase."
Lao-Sirieix S.H., Nookala R.K., Roversi P., Bell S.D., Pellegrini L.
Nat. Struct. Mol. Biol. 12:1137-1144(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.33 ANGSTROMS) OF 1-212, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y18930 Genomic DNA. Translation: CAB57742.1.
AE006641 Genomic DNA. Translation: AAK40874.1.
PIRC90202.
RefSeqNP_342084.1. NC_002754.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZT2X-ray3.33B/D1-212[»]
ProteinModelPortalQ9UWW1.
SMRQ9UWW1. Positions 3-209.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-42888N.
IntActQ9UWW1. 2 interactions.
MINTMINT-1896335.
STRING273057.SSO0557.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK40874; AAK40874; SSO0557.
GeneID1454841.
KEGGsso:SSO0557.

Phylogenomic databases

eggNOGCOG2219.
HOGENOMHOG000108364.
KOK02685.
OMAHHERFTI.
ProtClustDBPRK09568.

Enzyme and pathway databases

BioCycSSOL273057:GCH2-536-MONOMER.

Family and domain databases

HAMAPMF_00701. DNA_primase_lrg_arc.
InterProIPR023642. DNA_primase_lsu_arc.
IPR007238. DNA_primase_lsu_euk/arc.
[Graphical view]
PfamPF04104. DNA_primase_lrg. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9UWW1.

Entry information

Entry namePRIL_SULSO
AccessionPrimary (citable) accession number: Q9UWW1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: May 1, 2000
Last modified: February 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references