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Q9UWW1

- PRIL_SULSO

UniProt

Q9UWW1 - PRIL_SULSO

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Protein

DNA primase large subunit PriL

Gene

priL

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.1 PublicationUniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.1 PublicationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi216 – 2161Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi280 – 2801Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi289 – 2891Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi293 – 2931Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. DNA primase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Transcription

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-536-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase large subunit PriLUniRule annotation
Gene namesi
Name:priLUniRule annotation
Synonyms:priB
Ordered Locus Names:SSO0557
ORF Names:C21_042
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001974: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 307307DNA primase large subunit PriLPRO_0000046787Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a small subunit (PriS) and a large subunit (PriL).2 PublicationsUniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
ORF-c40_009Q9UXG03EBI-8081454,EBI-8081318From a different organism.

Protein-protein interaction databases

DIPiDIP-42888N.
IntActiQ9UWW1. 2 interactions.
MINTiMINT-1896335.
STRINGi273057.SSO0557.

Structurei

Secondary structure

1
307
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni5 – 73Combined sources
Helixi9 – 113Combined sources
Helixi14 – 196Combined sources
Helixi27 – 337Combined sources
Helixi36 – 5015Combined sources
Helixi57 – 593Combined sources
Beta strandi60 – 623Combined sources
Helixi63 – 7715Combined sources
Helixi80 – 9819Combined sources
Helixi103 – 11311Combined sources
Beta strandi118 – 1269Combined sources
Beta strandi128 – 14114Combined sources
Helixi142 – 1487Combined sources
Turni149 – 1513Combined sources
Helixi154 – 1563Combined sources
Beta strandi167 – 1704Combined sources
Helixi172 – 19019Combined sources
Helixi192 – 1954Combined sources
Helixi201 – 2088Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZT2X-ray3.33B/D1-212[»]
ProteinModelPortaliQ9UWW1.
SMRiQ9UWW1. Positions 3-209.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UWW1.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic-type primase large subunit family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2219.
HOGENOMiHOG000108364.
InParanoidiQ9UWW1.
KOiK02685.
OMAiRCHDLEG.

Family and domain databases

HAMAPiMF_00701. DNA_primase_lrg_arc.
InterProiIPR023642. DNA_primase_lsu_arc.
IPR007238. DNA_primase_lsu_euk/arc.
[Graphical view]
PfamiPF04104. DNA_primase_lrg. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UWW1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALDVKKYPF IKSLDDELKK YGGGITLTDL LLNSTTLIDQ AKDRIQKTKS
60 70 80 90 100
GDELPHYVSY NEPVLVFYTT LLSLAILNDV KLIRRYAYAE AKQFRSLLHT
110 120 130 140 150
ENEENLLEIS KLLDLKINRC DPIKFYLEKK RRIIQKEFCV HFIDYLKYTK
160 170 180 190 200
DLKEDWKLSG QILHKGYVYL DKNQLIGLIA ESIKSKIVEM IRPLNLKEIP
210 220 230 240 250
EKLKSLIERR GIIPPCIENI LAKEKLNEEE IRTLITFYID IGKGLSGIVS
260 270 280 290 300
IMKKYNVSNV EDLYRKYRGD KGTRYIVYSC AKMKQLGLCV SSCNVKNPLQ

LYFLSNE
Length:307
Mass (Da):35,734
Last modified:May 1, 2000 - v1
Checksum:i5EF09A913B4ABAC3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y18930 Genomic DNA. Translation: CAB57742.1.
AE006641 Genomic DNA. Translation: AAK40874.1.
PIRiC90202.
RefSeqiNP_342084.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40874; AAK40874; SSO0557.
GeneIDi1454841.
KEGGisso:SSO0557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y18930 Genomic DNA. Translation: CAB57742.1 .
AE006641 Genomic DNA. Translation: AAK40874.1 .
PIRi C90202.
RefSeqi NP_342084.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZT2 X-ray 3.33 B/D 1-212 [» ]
ProteinModelPortali Q9UWW1.
SMRi Q9UWW1. Positions 3-209.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-42888N.
IntActi Q9UWW1. 2 interactions.
MINTi MINT-1896335.
STRINGi 273057.SSO0557.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK40874 ; AAK40874 ; SSO0557 .
GeneIDi 1454841.
KEGGi sso:SSO0557.

Phylogenomic databases

eggNOGi COG2219.
HOGENOMi HOG000108364.
InParanoidi Q9UWW1.
KOi K02685.
OMAi RCHDLEG.

Enzyme and pathway databases

BioCyci SSOL273057:GCH2-536-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9UWW1.

Family and domain databases

HAMAPi MF_00701. DNA_primase_lrg_arc.
InterProi IPR023642. DNA_primase_lsu_arc.
IPR007238. DNA_primase_lsu_euk/arc.
[Graphical view ]
Pfami PF04104. DNA_primase_lrg. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
  3. "The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities."
    Lao-Sirieix S.H., Bell S.D.
    J. Mol. Biol. 344:1251-1263(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, GENE NAME.
  4. "An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis."
    Klinge S., Hirst J., Maman J.D., Krude T., Pellegrini L.
    Nat. Struct. Mol. Biol. 14:875-877(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IRON-SULFUR-BINDING, COFACTOR.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (3.33 ANGSTROMS) OF 1-212, SUBUNIT.

Entry informationi

Entry nameiPRIL_SULSO
AccessioniPrimary (citable) accession number: Q9UWW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3