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Q9UWW1

- PRIL_SULSO

UniProt

Q9UWW1 - PRIL_SULSO

Protein

DNA primase large subunit PriL

Gene

priL

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.1 PublicationUniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.1 PublicationUniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi216 – 2161Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi280 – 2801Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi289 – 2891Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi293 – 2931Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. DNA primase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication, Transcription

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciSSOL273057:GCH2-536-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA primase large subunit PriLUniRule annotation
    Gene namesi
    Name:priLUniRule annotation
    Synonyms:priB
    Ordered Locus Names:SSO0557
    ORF Names:C21_042
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001974: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 307307DNA primase large subunit PriLPRO_0000046787Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of a small subunit (PriS) and a large subunit (PriL).2 PublicationsUniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ORF-c40_009Q9UXG03EBI-8081454,EBI-8081318From a different organism.

    Protein-protein interaction databases

    DIPiDIP-42888N.
    IntActiQ9UWW1. 2 interactions.
    MINTiMINT-1896335.
    STRINGi273057.SSO0557.

    Structurei

    Secondary structure

    1
    307
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni5 – 73
    Helixi9 – 113
    Helixi14 – 196
    Helixi27 – 337
    Helixi36 – 5015
    Helixi57 – 593
    Beta strandi60 – 623
    Helixi63 – 7715
    Helixi80 – 9819
    Helixi103 – 11311
    Beta strandi118 – 1269
    Beta strandi128 – 14114
    Helixi142 – 1487
    Turni149 – 1513
    Helixi154 – 1563
    Beta strandi167 – 1704
    Helixi172 – 19019
    Helixi192 – 1954
    Helixi201 – 2088

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZT2X-ray3.33B/D1-212[»]
    ProteinModelPortaliQ9UWW1.
    SMRiQ9UWW1. Positions 3-209.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9UWW1.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eukaryotic-type primase large subunit family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2219.
    HOGENOMiHOG000108364.
    KOiK02685.
    OMAiRCHDLEG.

    Family and domain databases

    HAMAPiMF_00701. DNA_primase_lrg_arc.
    InterProiIPR023642. DNA_primase_lsu_arc.
    IPR007238. DNA_primase_lsu_euk/arc.
    [Graphical view]
    PfamiPF04104. DNA_primase_lrg. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9UWW1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALDVKKYPF IKSLDDELKK YGGGITLTDL LLNSTTLIDQ AKDRIQKTKS    50
    GDELPHYVSY NEPVLVFYTT LLSLAILNDV KLIRRYAYAE AKQFRSLLHT 100
    ENEENLLEIS KLLDLKINRC DPIKFYLEKK RRIIQKEFCV HFIDYLKYTK 150
    DLKEDWKLSG QILHKGYVYL DKNQLIGLIA ESIKSKIVEM IRPLNLKEIP 200
    EKLKSLIERR GIIPPCIENI LAKEKLNEEE IRTLITFYID IGKGLSGIVS 250
    IMKKYNVSNV EDLYRKYRGD KGTRYIVYSC AKMKQLGLCV SSCNVKNPLQ 300
    LYFLSNE 307
    Length:307
    Mass (Da):35,734
    Last modified:May 1, 2000 - v1
    Checksum:i5EF09A913B4ABAC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y18930 Genomic DNA. Translation: CAB57742.1.
    AE006641 Genomic DNA. Translation: AAK40874.1.
    PIRiC90202.
    RefSeqiNP_342084.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK40874; AAK40874; SSO0557.
    GeneIDi1454841.
    KEGGisso:SSO0557.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y18930 Genomic DNA. Translation: CAB57742.1 .
    AE006641 Genomic DNA. Translation: AAK40874.1 .
    PIRi C90202.
    RefSeqi NP_342084.1. NC_002754.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZT2 X-ray 3.33 B/D 1-212 [» ]
    ProteinModelPortali Q9UWW1.
    SMRi Q9UWW1. Positions 3-209.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-42888N.
    IntActi Q9UWW1. 2 interactions.
    MINTi MINT-1896335.
    STRINGi 273057.SSO0557.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK40874 ; AAK40874 ; SSO0557 .
    GeneIDi 1454841.
    KEGGi sso:SSO0557.

    Phylogenomic databases

    eggNOGi COG2219.
    HOGENOMi HOG000108364.
    KOi K02685.
    OMAi RCHDLEG.

    Enzyme and pathway databases

    BioCyci SSOL273057:GCH2-536-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q9UWW1.

    Family and domain databases

    HAMAPi MF_00701. DNA_primase_lrg_arc.
    InterProi IPR023642. DNA_primase_lsu_arc.
    IPR007238. DNA_primase_lsu_euk/arc.
    [Graphical view ]
    Pfami PF04104. DNA_primase_lrg. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
    3. "The heterodimeric primase of the hyperthermophilic archaeon Sulfolobus solfataricus possesses DNA and RNA primase, polymerase and 3'-terminal nucleotidyl transferase activities."
      Lao-Sirieix S.H., Bell S.D.
      J. Mol. Biol. 344:1251-1263(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, GENE NAME.
    4. "An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis."
      Klinge S., Hirst J., Maman J.D., Krude T., Pellegrini L.
      Nat. Struct. Mol. Biol. 14:875-877(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IRON-SULFUR-BINDING, COFACTOR.
    5. Cited for: X-RAY CRYSTALLOGRAPHY (3.33 ANGSTROMS) OF 1-212, SUBUNIT.

    Entry informationi

    Entry nameiPRIL_SULSO
    AccessioniPrimary (citable) accession number: Q9UWW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2003
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3