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Protein

DNA primase large subunit PriL

Gene

priL

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair. Possesses a template-independent 3'-terminal nucleotidyl transferase activity.UniRule annotation1 Publication

Cofactori

[4Fe-4S] clusterUniRule annotation1 PublicationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi216Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi280Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi289Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi293Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Transcription

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.7.B16. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase large subunit PriLUniRule annotation
Gene namesi
Name:priLUniRule annotation
Synonyms:priB
Ordered Locus Names:SSO0557
ORF Names:C21_042
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000467871 – 307DNA primase large subunit PriLAdd BLAST307

Interactioni

Subunit structurei

Heterodimer of a small subunit (PriS) and a large subunit (PriL).UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ORF-c40_009Q9UXG03EBI-8081454,EBI-8081318From a different organism.

Protein-protein interaction databases

DIPiDIP-42888N.
IntActiQ9UWW1. 2 interactors.
MINTiMINT-1896335.
STRINGi273057.SSO0557.

Structurei

Secondary structure

1307
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni5 – 7Combined sources3
Helixi9 – 11Combined sources3
Helixi14 – 19Combined sources6
Helixi27 – 33Combined sources7
Helixi36 – 50Combined sources15
Helixi57 – 59Combined sources3
Beta strandi60 – 62Combined sources3
Helixi63 – 77Combined sources15
Helixi80 – 98Combined sources19
Helixi103 – 113Combined sources11
Beta strandi118 – 126Combined sources9
Beta strandi128 – 141Combined sources14
Helixi142 – 148Combined sources7
Turni149 – 151Combined sources3
Helixi154 – 156Combined sources3
Beta strandi167 – 170Combined sources4
Helixi172 – 190Combined sources19
Helixi192 – 195Combined sources4
Helixi201 – 208Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZT2X-ray3.33B/D1-212[»]
ProteinModelPortaliQ9UWW1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UWW1.

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic-type primase large subunit family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG03013. Archaea.
COG2219. LUCA.
HOGENOMiHOG000108364.
InParanoidiQ9UWW1.
KOiK18882.
OMAiHHERFTI.

Family and domain databases

HAMAPiMF_00701. DNA_primase_lrg_arc. 1 hit.
InterProiIPR007238. DNA_primase_lsu_euk/arc.
IPR023642. DNA_primase_lsu_PriL.
[Graphical view]
PfamiPF04104. DNA_primase_lrg. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UWW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALDVKKYPF IKSLDDELKK YGGGITLTDL LLNSTTLIDQ AKDRIQKTKS
60 70 80 90 100
GDELPHYVSY NEPVLVFYTT LLSLAILNDV KLIRRYAYAE AKQFRSLLHT
110 120 130 140 150
ENEENLLEIS KLLDLKINRC DPIKFYLEKK RRIIQKEFCV HFIDYLKYTK
160 170 180 190 200
DLKEDWKLSG QILHKGYVYL DKNQLIGLIA ESIKSKIVEM IRPLNLKEIP
210 220 230 240 250
EKLKSLIERR GIIPPCIENI LAKEKLNEEE IRTLITFYID IGKGLSGIVS
260 270 280 290 300
IMKKYNVSNV EDLYRKYRGD KGTRYIVYSC AKMKQLGLCV SSCNVKNPLQ

LYFLSNE
Length:307
Mass (Da):35,734
Last modified:May 1, 2000 - v1
Checksum:i5EF09A913B4ABAC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18930 Genomic DNA. Translation: CAB57742.1.
AE006641 Genomic DNA. Translation: AAK40874.1.
PIRiC90202.
RefSeqiWP_009991067.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK40874; AAK40874; SSO0557.
GeneIDi27426865.
KEGGisso:SSO0557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18930 Genomic DNA. Translation: CAB57742.1.
AE006641 Genomic DNA. Translation: AAK40874.1.
PIRiC90202.
RefSeqiWP_009991067.1. NC_002754.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZT2X-ray3.33B/D1-212[»]
ProteinModelPortaliQ9UWW1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-42888N.
IntActiQ9UWW1. 2 interactors.
MINTiMINT-1896335.
STRINGi273057.SSO0557.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK40874; AAK40874; SSO0557.
GeneIDi27426865.
KEGGisso:SSO0557.

Phylogenomic databases

eggNOGiarCOG03013. Archaea.
COG2219. LUCA.
HOGENOMiHOG000108364.
InParanoidiQ9UWW1.
KOiK18882.
OMAiHHERFTI.

Enzyme and pathway databases

BRENDAi2.7.7.B16. 6163.

Miscellaneous databases

EvolutionaryTraceiQ9UWW1.

Family and domain databases

HAMAPiMF_00701. DNA_primase_lrg_arc. 1 hit.
InterProiIPR007238. DNA_primase_lsu_euk/arc.
IPR023642. DNA_primase_lsu_PriL.
[Graphical view]
PfamiPF04104. DNA_primase_lrg. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRIL_SULSO
AccessioniPrimary (citable) accession number: Q9UWW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.