Q9UWN9 (Q9UWN9_SULSH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Name:	TreZ EMBL AAF17553.1
Synonyms:	treZ EMBL AAM81589.1

Organism

Sulfolobus shibatae EMBL AAF17553.1

Taxonomic identifier

2286 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	253	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	284	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	378	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	Q9UWN9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8C0F0F38A5AE2846	FASTA	559	64,619
	10 20 30 40 50 60 MTFGYKIDGN EVIFTLWAPY QKSVKLKVLE KGLYEMERDD KGYFTITLNN VKVGDRYKYV 70 80 90 100 110 120 LDDASEIPDP ASRYQPEGVH GPSQIIQESK EFNNETFLKK EDLIIYEIHV GTFAPEGTFE 130 140 150 160 170 180 GVIRKLDYLK DLGITAIEIM PIAQFPGKRD WGYDGVYLYA VQNSYGGPEG FRKLVDEAHM 190 200 210 220 230 240 KGLGVILDVV YNHVGPEGNY MVKLGPYFSQ KYKTPWGLTF NFDDAESDEV RKFILENVEY 250 260 270 280 290 300 WIKEYNVDGF RLDAVHSIID TSPKHILEEI ADVVHKYNRI VIAESDLNDP RVVNPKEKCG 310 320 330 340 350 360 YNIDAQWVDD FHHSIHAYLT GERQGYYLDF GNLDNIVKSY KDVFVYDGKY SKFRRKTHGK 370 380 390 400 410 420 PVGELDGCNF VVYIQNHDQV GNRGKGERII KLVDKESYKN AAALYILSSI ISMIFMGEEY 430 440 450 460 470 480 GEQKPFYFFS DFSDTKLIQG LREGRKKENG QDTDPQDEST FNASKLSWKI DEEIFSFYKI 490 500 510 520 530 540 LIKMRNELSI ACDRRVYVEN GENWLIIKGR EYLSLCVFSR SSIEVKYSGT LLLSSNNSFP 550 QHIEEGQYEF DKGFALYKL

References

[1]	"Maltooligosyl trehalose trehalohydrolase from Sulfolobus shibatae." Weiting Y., Wei C., Hui W., Li L., Cheng J. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Cloning and sequence analysis of genes encoding enzymes for trehalose biosynthesis from thermophilic archaebacterium Sulfolobus shibatae B12." Wu J., Yu W.T., Chen W., Liu L., Wang H., Wang S.X. Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: B12 EMBL AAM81589.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF201335 Genomic DNA. Translation: AAF17553.1. AY121427 Genomic DNA. Translation: AAM81589.1.
3D structure databases
HSSP	HSSP built from PDB template 1EH9 based on UniProtKB Q55088.
ProteinModelPortal	Q9UWN9.
SMR	Q9UWN9. Positions 2-558.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR015156. Maltooligo_trehalose_arc_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF09071. Alpha-amyl_C. 1 hit. PF00128. Alpha-amylase. 2 hits. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q9UWN9_SULSH

Accession

Primary (citable) accession number: Q9UWN9

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)