Q9UW86 (PP1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1
EC=3.1.3.16

Gene names

Name:	ppp-1
ORF Names:	NCU00043

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage checkpoint Inferred from electronic annotation. Source: EnsemblFungi DNA replication checkpoint Inferred from electronic annotation. Source: EnsemblFungi ascospore formation Inferred from electronic annotation. Source: EnsemblFungi cell budding Inferred from electronic annotation. Source: EnsemblFungi cellular ion homeostasis Inferred from electronic annotation. Source: EnsemblFungi chromosome segregation Inferred from electronic annotation. Source: EnsemblFungi glycogen metabolic process Inferred from electronic annotation. Source: EnsemblFungi histone dephosphorylation Inferred from electronic annotation. Source: EnsemblFungi meiosis Inferred from electronic annotation. Source: EnsemblFungi mitotic spindle assembly checkpoint Inferred from electronic annotation. Source: EnsemblFungi protein localization to kinetochore Inferred from electronic annotation. Source: EnsemblFungi rRNA processing Inferred from electronic annotation. Source: EnsemblFungi regulation of carbohydrate metabolic process Inferred from electronic annotation. Source: EnsemblFungi regulation of cell shape during vegetative growth phase Inferred from electronic annotation. Source: EnsemblFungi replication fork processing Inferred from electronic annotation. Source: EnsemblFungi response to heat Inferred from electronic annotation. Source: EnsemblFungi termination of RNA polymerase II transcription, exosome-dependent Inferred from electronic annotation. Source: EnsemblFungi termination of RNA polymerase II transcription, poly(A)-coupled Inferred from electronic annotation. Source: EnsemblFungi
Cellular_component	cellular bud neck Inferred from electronic annotation. Source: EnsemblFungi condensed nuclear chromosome kinetochore Inferred from electronic annotation. Source: EnsemblFungi mRNA cleavage and polyadenylation specificity factor complex Inferred from electronic annotation. Source: EnsemblFungi mating projection base Inferred from electronic annotation. Source: EnsemblFungi nucleolus Inferred from electronic annotation. Source: EnsemblFungi protein phosphatase type 1 complex Inferred from electronic annotation. Source: EnsemblFungi spindle pole body Inferred from electronic annotation. Source: EnsemblFungi
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: EnsemblFungi
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Serine/threonine-protein phosphatase PP1		PRO_0000058817
Sites
	Active site	125	1	Proton donor By similarity
	Metal binding	64	1	Iron By similarity
	Metal binding	66	1	Iron By similarity
	Metal binding	92	1	Iron By similarity
	Metal binding	92	1	Manganese By similarity
	Metal binding	124	1	Manganese By similarity
	Metal binding	173	1	Manganese By similarity
	Metal binding	248	1	Manganese By similarity
Experimental info
	Sequence conflict	154	1	D → E in AAC05275. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9UW86 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 333A728F20860AB1	FASTA	308	35,568
	10 20 30 40 50 60 MADHTEVDLD SIIDRLLEVR GSRPGKQVQL LEAEIRYLCT KAREIFISQP ILLELEAPIK 70 80 90 100 110 120 ICGDIHGQYY DLLRLFEYGG FPPEANYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFI 130 140 150 160 170 180 LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIIDEKIF TMHGGLSPDL 190 200 210 220 230 240 NSMEQIRRVM RPTDIPDCGL LCDLLWSDPD KDITGWSEND RGVSFTFGPD VVSRFLQKHD 250 260 270 280 290 300 MDLICRAHQV VEDGYEFFSK RQLVTLFSAP NYCGEFDNAG AMMSVDESLL CSFQILKPAE KKQKFGRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Searching for the role of protein phosphatases in eukaryotic microorganisms." da-Silva A.M., Zapella P.D.A., Andrioli L.P.M., Campanha R.B., Fiorini L.C., Etchebehere L.C., da-Costa-Maia J.C., Terenzi H.F. Braz. J. Med. Biol. Res. 32:835-839(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"Expression of protein phosphatase 1 during the asexual development of Neurospora crassa." Zeke T., Kokai E., Szoor B., Yatzkan E., Yarden O., Szirak K., Feher Z., Bagossi P., Gergely P., Dombradi V. Comp. Biochem. Physiol. 134B:161-170(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 74-OR23-1VA / FGSC 2489.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF049853 mRNA. Translation: AAC05275.1. AF124149 Genomic DNA. Translation: AAD47567.1. AABX02000001 Genomic DNA. Translation: EAA26918.2.
RefSeq	XP_956154.2. XM_951061.2.
UniGene	Ncr.4075.
3D structure databases
ProteinModelPortal	Q9UW86.
SMR	Q9UW86. Positions 1-305.
ModBase	Search...
Protein-protein interaction databases
STRING	5141.NCU00043.1.
Proteomic databases
PRIDE	Q9UW86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000000314; EFNCRP00000000314; EFNCRG00000000314.
GeneID	3872306.
KEGG	ncr:NCU00043.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172697.
KO	K06269.
OrthoDB	EOG4MGWH2.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP1_NEUCR

Accession

Primary (citable) accession number: Q9UW86
Secondary accession number(s): O59927, Q7RV51

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program