Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Poly(A) polymerase PAPa

Gene

PAPA

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (By similarity).By similarity

Miscellaneous

The C.albicans mating-type-like (MTL) locus contains, in addition to the genes for the regulatory proteins (MTLA1, MTLA2, MTLALPHA1 and MTLALPHA2), a and alpha idiomorphs of a phosphatidylinositol kinase (PIKA and PIKALPHA), a poly(A) polymerase (PAPA and PAPALPHA) and an oxysterol binding protein-like protein (OBPA and OBPALPHA).

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Magnesium 1; catalyticBy similarity1
Metal bindingi99Magnesium 2; catalyticBy similarity1
Metal bindingi101Magnesium 1; catalyticBy similarity1
Metal bindingi101Magnesium 2; catalyticBy similarity1
Metal bindingi153Magnesium 2; catalyticBy similarity1
Binding sitei153ATPBy similarity1
Binding sitei214ATPBy similarity1
Binding sitei223ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 88ATPBy similarity3
Nucleotide bindingi99 – 101ATPBy similarity3
Nucleotide bindingi232 – 233ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • polynucleotide adenylyltransferase activity Source: CGD
  • RNA binding Source: CGD

GO - Biological processi

  • mRNA polyadenylation Source: CGD
  • single-species biofilm formation on inanimate substrate Source: CGD

Keywordsi

Molecular functionRNA-binding, Transferase
Biological processmRNA processing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase PAPa (EC:2.7.7.19)
Alternative name(s):
Polynucleotide adenylyltransferase a
Gene namesi
Name:PAPA
Synonyms:PAP99
Ordered Locus Names:CAALFM_C501780WA
ORF Names:CaO19.3197
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome 5

Organism-specific databases

CGDiCAL0000194576 PAP1
EuPathDBiFungiDB:C5_01780W_A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516191 – 555Poly(A) polymerase PAPaAdd BLAST555

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei139Interaction with RNABy similarity1
Sitei144Interaction with RNABy similarity1
Sitei313Interaction with RNABy similarity1
Sitei314Interaction with RNABy similarity1
Sitei386Interaction with RNABy similarity1
Sitei391Interaction with RNABy similarity1
Sitei487Interaction with RNABy similarity1

Structurei

3D structure databases

ProteinModelPortaliQ9UW26
SMRiQ9UW26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

InParanoidiQ9UW26
KOiK14376
OrthoDBiEOG092C1VMQ

Family and domain databases

InterProiView protein in InterPro
IPR011068 NuclTrfase_I-like_C
IPR007012 PolA_pol_cen_dom
IPR007010 PolA_pol_RNA-bd_dom
IPR014492 PolyA_polymerase
IPR002934 Polymerase_NTP_transf_dom
PANTHERiPTHR10682 PTHR10682, 1 hit
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF04928 PAP_central, 1 hit
PF04926 PAP_RNA-bind, 1 hit
PIRSFiPIRSF018425 PolyA_polymerase, 1 hit
SUPFAMiSSF55003 SSF55003, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UW26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNQAYGVTP PISVANSTPK ENELNDSLIK ELKSRGSFES ETATKKRVEV
60 70 80 90 100
LNILQSMTEE FVYKVSIKKN ISEGMARDVG GKIFTFGSYR LGVYGPGSDI
110 120 130 140 150
DTLVVVPKHV SRNDFFEVFY ELLKGRSELE EIAPVPDAFV PIIKIEFAGI
160 170 180 190 200
SIDLIFARLD IPRVPRDLTL DDKNLLKNID EKDLRALNGT RVTDEILQLV
210 220 230 240 250
PKPTVFKHAL RCIKMWAQQR AVYGNIFGFP GGVAWAMLVA RICQLYPNAV
260 270 280 290 300
SAVIVEKFFH IYSQWAWPQP VLLKQIEDGP LQVRVWNPRL YALDRQHRMP
310 320 330 340 350
VITPAYPSMC ATHNITSSTQ KVILSEFQRG IELMNDINVG KKSWSDLLER
360 370 380 390 400
HDFFFRYKFY LCIVAATRST YAEHLKYSGM VESKLRLLVQ KLELVEGIEL
410 420 430 440 450
AHPYVKSFEN GYYCDNAEEA HEIMNLYGTS KGDDRVKGVL HAENNDNNKE
460 470 480 490 500
NVENKVELHM TKLFIGLKLD LSKEGEKKLD IQYPCAEFFN ICKGWQDFDS
510 520 530 540 550
EKHFIQIKNV KLYDLSDDVY VDGETRPIKI AKRKRAVSKN EGKKKPKSVG

TVSAA
Length:555
Mass (Da):63,289
Last modified:May 1, 2000 - v1
Checksum:i5C76DA500616042A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti447N → K in BAA36217 (Ref. 2) Curated1
Sequence conflicti506Q → R in BAA36217 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167162 Genomic DNA Translation: AAD51407.1
AB021667 Genomic DNA Translation: BAA36217.1
CP017627 Genomic DNA Translation: AOW29609.1
RefSeqiXP_714613.1, XM_709520.1

Genome annotation databases

EnsemblFungiiAOW29609; AOW29609; CAALFM_C501780WA
GeneIDi3643763
KEGGical:CAALFM_C501780WA

Similar proteinsi

Entry informationi

Entry nameiPAPA_CANAL
AccessioniPrimary (citable) accession number: Q9UW26
Secondary accession number(s): A0A1D8PN98, O93834, Q59YI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health