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Q9UW26 (PAPA_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase PAPa

EC=2.7.7.19
Alternative name(s):
Polynucleotide adenylyltransferase a
Gene names
Name:PAPA
Synonyms:PAP99
ORF Names:CaO19.3197
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length555 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor By similarity.

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Binds 2 magnesium ions. Also active with manganese By similarity.

Subcellular location

Nucleus By similarity.

Miscellaneous

The C.albicans mating-type-like (MTL) locus contains, in addition to the genes for the regulatory proteins (MTLA1, MTLA2, MTLALPHA1 and MTLALPHA2), a and alpha idiomorphs of a phosphatidylinositol kinase (PIKA and PIKALPHA), a poly(A) polymerase (PAPA and PAPALPHA) and an oxysterol binding protein-like protein (OBPA and OBPALPHA).

Sequence similarities

Belongs to the poly(A) polymerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 555555Poly(A) polymerase PAPa
PRO_0000051619

Regions

Nucleotide binding86 – 883ATP By similarity
Nucleotide binding99 – 1013ATP By similarity
Nucleotide binding232 – 2332ATP By similarity

Sites

Metal binding991Magnesium 1; catalytic By similarity
Metal binding991Magnesium 2; catalytic By similarity
Metal binding1011Magnesium 1; catalytic By similarity
Metal binding1011Magnesium 2; catalytic By similarity
Metal binding1531Magnesium 2; catalytic By similarity
Binding site1531ATP By similarity
Binding site2141ATP By similarity
Binding site2231ATP By similarity
Site1391Interaction with RNA By similarity
Site1441Interaction with RNA By similarity
Site3131Interaction with RNA By similarity
Site3141Interaction with RNA By similarity
Site3861Interaction with RNA By similarity
Site3911Interaction with RNA By similarity
Site4871Interaction with RNA By similarity

Experimental info

Sequence conflict4471N → K in BAA36217. Ref.2
Sequence conflict5061Q → R in BAA36217. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9UW26 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5C76DA500616042A

FASTA55563,289
        10         20         30         40         50         60 
MNNQAYGVTP PISVANSTPK ENELNDSLIK ELKSRGSFES ETATKKRVEV LNILQSMTEE 

        70         80         90        100        110        120 
FVYKVSIKKN ISEGMARDVG GKIFTFGSYR LGVYGPGSDI DTLVVVPKHV SRNDFFEVFY 

       130        140        150        160        170        180 
ELLKGRSELE EIAPVPDAFV PIIKIEFAGI SIDLIFARLD IPRVPRDLTL DDKNLLKNID 

       190        200        210        220        230        240 
EKDLRALNGT RVTDEILQLV PKPTVFKHAL RCIKMWAQQR AVYGNIFGFP GGVAWAMLVA 

       250        260        270        280        290        300 
RICQLYPNAV SAVIVEKFFH IYSQWAWPQP VLLKQIEDGP LQVRVWNPRL YALDRQHRMP 

       310        320        330        340        350        360 
VITPAYPSMC ATHNITSSTQ KVILSEFQRG IELMNDINVG KKSWSDLLER HDFFFRYKFY 

       370        380        390        400        410        420 
LCIVAATRST YAEHLKYSGM VESKLRLLVQ KLELVEGIEL AHPYVKSFEN GYYCDNAEEA 

       430        440        450        460        470        480 
HEIMNLYGTS KGDDRVKGVL HAENNDNNKE NVENKVELHM TKLFIGLKLD LSKEGEKKLD 

       490        500        510        520        530        540 
IQYPCAEFFN ICKGWQDFDS EKHFIQIKNV KLYDLSDDVY VDGETRPIKI AKRKRAVSKN 

       550 
EGKKKPKSVG TVSAA 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans."
Hull C.M., Johnson A.D.
Science 285:1271-1275(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.
[2]"Candida albicans PAP99 gene, a putative homolog of the C.albicans PAP1 gene."
Nagahashi S., Ishii N., Aoki Y., Arisawa M.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10259 / CBS 5796 / DSM 5817 / JCM 2078 / NBRC 1060.
[3]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF167162 Genomic DNA. Translation: AAD51407.1.
AB021667 Genomic DNA. Translation: BAA36217.1.
AACQ01000100 Genomic DNA. Translation: EAK95563.1.
RefSeqXP_714613.1. XM_709520.1.

3D structure databases

ProteinModelPortalQ9UW26.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0002451.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3643763.
KEGGcal:CaO19.3197.

Organism-specific databases

CGDCAL0002451. PAP1.

Phylogenomic databases

eggNOGCOG5186.
KOK14376.
OrthoDBEOG7M6DHG.

Family and domain databases

Gene3D3.30.70.590. 1 hit.
InterProIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMSSF55003. SSF55003. 1 hit.
ProtoNetSearch...

Entry information

Entry namePAPA_CANAL
AccessionPrimary (citable) accession number: Q9UW26
Secondary accession number(s): O93834, Q59YI3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 2000
Last modified: November 13, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Candida albicans

Candida albicans: entries and gene names