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Protein

Poly(A) polymerase PAPa

Gene

PAPA

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's. May acquire specificity through interaction with a cleavage and polyadenylation factor (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Magnesium 1; catalyticBy similarity1
Metal bindingi99Magnesium 2; catalyticBy similarity1
Metal bindingi101Magnesium 1; catalyticBy similarity1
Metal bindingi101Magnesium 2; catalyticBy similarity1
Metal bindingi153Magnesium 2; catalyticBy similarity1
Binding sitei153ATPBy similarity1
Binding sitei214ATPBy similarity1
Binding sitei223ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 88ATPBy similarity3
Nucleotide bindingi99 – 101ATPBy similarity3
Nucleotide bindingi232 – 233ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • polynucleotide adenylyltransferase activity Source: CGD
  • RNA binding Source: CGD

GO - Biological processi

  • mRNA polyadenylation Source: CGD
  • single-species biofilm formation on inanimate substrate Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase PAPa (EC:2.7.7.19)
Alternative name(s):
Polynucleotide adenylyltransferase a
Gene namesi
Name:PAPA
Synonyms:PAP99
ORF Names:CaO19.3197
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0000194576. PAP1.
EuPathDBiFungiDB:C5_01780W_A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516191 – 555Poly(A) polymerase PAPaAdd BLAST555

Proteomic databases

PRIDEiQ9UW26.

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei139Interaction with RNABy similarity1
Sitei144Interaction with RNABy similarity1
Sitei313Interaction with RNABy similarity1
Sitei314Interaction with RNABy similarity1
Sitei386Interaction with RNABy similarity1
Sitei391Interaction with RNABy similarity1
Sitei487Interaction with RNABy similarity1

Structurei

3D structure databases

ProteinModelPortaliQ9UW26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

InParanoidiQ9UW26.
KOiK14376.
OrthoDBiEOG092C1VMQ.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 2 hits.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UW26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNQAYGVTP PISVANSTPK ENELNDSLIK ELKSRGSFES ETATKKRVEV
60 70 80 90 100
LNILQSMTEE FVYKVSIKKN ISEGMARDVG GKIFTFGSYR LGVYGPGSDI
110 120 130 140 150
DTLVVVPKHV SRNDFFEVFY ELLKGRSELE EIAPVPDAFV PIIKIEFAGI
160 170 180 190 200
SIDLIFARLD IPRVPRDLTL DDKNLLKNID EKDLRALNGT RVTDEILQLV
210 220 230 240 250
PKPTVFKHAL RCIKMWAQQR AVYGNIFGFP GGVAWAMLVA RICQLYPNAV
260 270 280 290 300
SAVIVEKFFH IYSQWAWPQP VLLKQIEDGP LQVRVWNPRL YALDRQHRMP
310 320 330 340 350
VITPAYPSMC ATHNITSSTQ KVILSEFQRG IELMNDINVG KKSWSDLLER
360 370 380 390 400
HDFFFRYKFY LCIVAATRST YAEHLKYSGM VESKLRLLVQ KLELVEGIEL
410 420 430 440 450
AHPYVKSFEN GYYCDNAEEA HEIMNLYGTS KGDDRVKGVL HAENNDNNKE
460 470 480 490 500
NVENKVELHM TKLFIGLKLD LSKEGEKKLD IQYPCAEFFN ICKGWQDFDS
510 520 530 540 550
EKHFIQIKNV KLYDLSDDVY VDGETRPIKI AKRKRAVSKN EGKKKPKSVG

TVSAA
Length:555
Mass (Da):63,289
Last modified:May 1, 2000 - v1
Checksum:i5C76DA500616042A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti447N → K in BAA36217 (Ref. 2) Curated1
Sequence conflicti506Q → R in BAA36217 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167162 Genomic DNA. Translation: AAD51407.1.
AB021667 Genomic DNA. Translation: BAA36217.1.
AACQ01000100 Genomic DNA. Translation: EAK95563.1.
RefSeqiXP_714613.1. XM_709520.1.

Genome annotation databases

EnsemblFungiiEAK95563; EAK95563; CaO19.3197.
GeneIDi3643763.
KEGGical:CaO19.3197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167162 Genomic DNA. Translation: AAD51407.1.
AB021667 Genomic DNA. Translation: BAA36217.1.
AACQ01000100 Genomic DNA. Translation: EAK95563.1.
RefSeqiXP_714613.1. XM_709520.1.

3D structure databases

ProteinModelPortaliQ9UW26.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9UW26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAK95563; EAK95563; CaO19.3197.
GeneIDi3643763.
KEGGical:CaO19.3197.

Organism-specific databases

CGDiCAL0000194576. PAP1.
EuPathDBiFungiDB:C5_01780W_A.

Phylogenomic databases

InParanoidiQ9UW26.
KOiK14376.
OrthoDBiEOG092C1VMQ.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
[Graphical view]
PANTHERiPTHR10682. PTHR10682. 2 hits.
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAPA_CANAL
AccessioniPrimary (citable) accession number: Q9UW26
Secondary accession number(s): O93834, Q59YI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The C.albicans mating-type-like (MTL) locus contains, in addition to the genes for the regulatory proteins (MTLA1, MTLA2, MTLALPHA1 and MTLALPHA2), a and alpha idiomorphs of a phosphatidylinositol kinase (PIKA and PIKALPHA), a poly(A) polymerase (PAPA and PAPALPHA) and an oxysterol binding protein-like protein (OBPA and OBPALPHA).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.