Q9UW20 (PIK1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-kinase PIK1alpha
Short name=PI4-kinase
Short name=PtdIns-4-kinase
EC=2.7.1.67

Gene names

Name:	PIKALPHA
Synonyms:	PIK1
ORF Names:	CaO19.10711

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	977 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts on phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Subcellular location	Nucleus By similarity.
Miscellaneous	The C.albicans mating-type-like (MTL) locus contains, in addition to the genes for the regulatory proteins (MTLA1, MTLA2, MTLALPHA1 and MTLALPHA2), a and alpha idiomorphs of a phosphatidylinositol kinase (PIKA and PIKALPHA), a poly(A) polymerase (PAPA and PAPALPHA) and an oxysterol binding protein-like protein (OBPA and OBPALPHA).
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 977	977	Phosphatidylinositol 4-kinase PIK1alpha		PRO_0000088832
Regions
	Domain	1 – 125	125	PIK helical
	Domain	703 – 974	272	PI3K/PI4K
Experimental info
	Sequence conflict	87	1	R → G in CAA09718. Ref.2
	Sequence conflict	582	1	S → A in CAA09718. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9UW20 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: C7899B61AC352AA4	FASTA	977	110,699
	10 20 30 40 50 60 MSADITETPN KQLLEQIRSP SFSLFNCIYE LKNHTDSIGI QHELVKKLYS FPYEDLQFFI 70 80 90 100 110 120 PQFVQLLVTY DSESMALEEF IITYSSRYPH FSLIVFWNLQ AYIFELKNEP ESRSFQAVRN 130 140 150 160 170 180 LITKIQNIMF NADQQTVKAP EFRENFLPAL VLCGAVASSV LLPSFKSYCL PMIKAQGKQQ 190 200 210 220 230 240 KSLVFKLVNF QKSLTKNLTL KNQRMSADIP KGSHSDDETA TSSSIKPSLS RSASVPRRNT 250 260 270 280 290 300 KKTSLSFSSD ESEAYTTDDD DNKTSIELEK DFYKIDLDGL SKEKSANFLE PEENLNVNTA 310 320 330 340 350 360 IKSKKRLSTL TSKVMTQPWN GIDGYNVNSQ SLPDLSKAEG RDLIPFISST ESETSLLYHN 370 380 390 400 410 420 NSISNDLQKN IPRQQKFSPG FDNVYLTKLL QVNYAKNETQ FIMALQNISI RLSQVPKEAR 430 440 450 460 470 480 LSALRAELSI INDTLLPSEI DIPQLLPITS NRNKKYHKIL KLNVNEASVL NSAERVPFLL 490 500 510 520 530 540 FIEYLSDEID FNPTTEYNQR IIARKKMNGA TSMTVKKINS FSEVADGNFE KENKIKSSTP 550 560 570 580 590 600 ETVSNIIYNE NTEEADLSEM PLDRKTTVSS DSFSPEMLVT PSITEQSKLS NFPSLNTKEV 610 620 630 640 650 660 STKVLADQMR IAAVMLQQLD SSGKANSEQS FLIKNRIVES MIALQDQFDS FDFEKLSQLQ 670 680 690 700 710 720 SDEPSAGERK LENDFKLGED WNTKKQRIKK SSAYGHLKNW DLCSVIAKNG DDLPQEAFAC 730 740 750 760 770 780 QLISMISNIW KKNNIPVWTK RMKILITSAN TGLVETITNA MSIHSIKKSF TEHSIKSGEN 790 800 810 820 830 840 SKGKIFTLLD YFHSVFGSPN STSFRTAQQN FAKSLAAYSI ICYVLQIKDR HNGNIMVDGD 850 860 870 880 890 900 GHIIHIDFGF LLSNSPGSVG FEAAPFKLTV EYVELLGGVD SEIYSQFVYL CKQCFKSLRD 910 920 930 940 950 960 NSEEIIEIVE LMQKDSTLPC FNNGENTSVL LKQRLQLQLN DEDTDQFVEN FLIGKSLGSM 970 YTRLYDQFQM ITQGIYS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans." Hull C.M., Johnson A.D. Science 285:1271-1275(1999) [PubMed: 10455055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"Cloning and sequencing of a C. albicans phosphatidylinositol 4-kinase." Gamo F.J., Garcia-Bustos J.F. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF167163 Genomic DNA. Translation: AAD51410.1. AJ011588 Genomic DNA. Translation: CAA09718.1. AACQ01000097 Genomic DNA. Translation: EAK95704.1.
RefSeq	XP_714748.1. XM_709655.1.
3D structure databases
ProteinModelPortal	Q9UW20.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3643606.
KEGG	cal:CaO19.10711.
Organism-specific databases
CGD	CAL0006639. PIKALPHA.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR011009. Kinase-like_dom. IPR021601. Phosphatidylinositol_kinase. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit. G3DSA:1.25.40.70. PI3Ka. 1 hit.
KO	K00888.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF11522. Pik1. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. False negative. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIK1_CANAL

Accession

Primary (citable) accession number: Q9UW20
Secondary accession number(s): O74714, Q59YW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program