Reviewed, UniProtKB/Swiss-Prot Q9UVX3 (NMT_ASPFU)

Names and origin

Protein names

Recommended name:
    Glycylpeptide N-tetradecanoyltransferase
    EC=2.3.1.97
Alternative name(s):
    Peptide N-myristoyltransferase
    Myristoyl-CoA:protein N-myristoyltransferase
      Short name=NMT

Gene names

Name:	nmt1
ORF Names:	AFUA_4G08070

Organism

Aspergillus fumigatus (Sartorya fumigata) [Complete proteome]

Taxonomic identifier

5085 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Glycylpeptide N-tetradecanoyltransferase		PRO_0000064235
Regions
	Region	82 – 85	4	Myristoyl CoA-binding By similarity
	Region	212 – 248	37	Myristoyl CoA-binding By similarity
Sites
	Active site	492	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UVX3-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 921C0C5FF805F000	FASTA	492	56,255
	10 20 30 40 50 60 MSDSKDRKGK APEGQSSEKK DGAVNITPQM AESLLENNPA LRNETAGMDK DKAAEAMRKM 70 80 90 100 110 120 NIAELLTGLS VSGKNQKDMA SYKFWQTQPV PRFDETSTDT GGPIKIIDPE KVSKEPDALL 130 140 150 160 170 180 EGFEWATLDL TNETELQELW DLLTYHYVED DNAMFRFRYS QSFLHWALMS PGWKKEWHVG 190 200 210 220 230 240 VRATKSRKLV ASICGVPTEI NVRNQKLKVV EINFLCIHKK LRSKRLTPVL IKEITRRCYL 250 260 270 280 290 300 NGIYQAIYTA GVVLPTPVSS CRYYHRPLDW LKLYEVGFSP LPAGSTKARQ ITKNHLPSTT 310 320 330 340 350 360 STPGLRPMEP KDIDTVHDLL QRYLSRFALN QAFTREEVDH WLVHKPETVK EQVVWAYVVE 370 380 390 400 410 420 DPETHKITDF FSFYNLESTV IQNPKHDNVR AAYLYYYATE TAFTNNMKAL KERLLMLMND 430 440 450 460 470 480 ALILAKKAHF DVFNALTLHD NPLFLEQLKF GAGDGQLHFY LYNYRTAPVP GGVNEKNLPD 490 EKRMGGVGIV ML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"N-myristoyl transferase." Sakata K., Hashido K., Aoki Y., Arisawa M. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB035414 mRNA. Translation: BAA87865.1. AAHF01000005 Genomic DNA. Translation: EAL89981.1.
RefSeq	XP_752019.1.
3D structure databases
HSSP	HSSP built from PDB template 1IIC based on UniProtKB P14743.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UVX3.
Genome annotation databases
GeneID	3508953.
GenomeReviews	Gene locus nmt1 in contig CM000172_GR.
KEGG	afm:AFUA_4G08070.
Phylogenomic databases
OMA	VEDDDSM.
Enzyme and pathway databases
BRENDA	2.3.1.97. 18841.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. Myristoyl_trans. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_ASPFU

Accession

Primary (citable) accession number: Q9UVX3
Secondary accession number(s): Q4WP53

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)