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Protein

6,7-dimethyl-8-ribityllumazine synthase

Gene

MGG_04626

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.

Catalytic activityi

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate.

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (MGG_04626)
  2. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei255-amino-6-(D-ribitylamino)uracil1
Active sitei127Proton donorSequence analysis1
Binding sitei1525-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygen1
Binding sitei1661-deoxy-L-glycero-tetrulose 4-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Riboflavin biosynthesis

Enzyme and pathway databases

BRENDAi2.5.1.78. 3152.
UniPathwayiUPA00275; UER00404.

Names & Taxonomyi

Protein namesi
Recommended name:
6,7-dimethyl-8-ribityllumazine synthase (EC:2.5.1.78)
Short name:
DMRL synthase
Short name:
LS
Short name:
Lumazine synthase
Gene namesi
ORF Names:MGG_04626
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiFungiDB:MGG_04626.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001348551 – 2006,7-dimethyl-8-ribityllumazine synthaseAdd BLAST200

Interactioni

Subunit structurei

Homopentamer.1 Publication

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 23Combined sources6
Helixi27 – 43Combined sources17
Helixi48 – 50Combined sources3
Beta strandi51 – 58Combined sources8
Helixi61 – 75Combined sources15
Beta strandi112 – 121Combined sources10
Helixi127 – 146Combined sources20
Beta strandi150 – 159Combined sources10
Helixi160 – 166Combined sources7
Helixi176 – 195Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C41X-ray3.10A/B/C/D/E/F/G/H/I/J1-200[»]
ProteinModelPortaliQ9UVT8.
SMRiQ9UVT8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UVT8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 615-amino-6-(D-ribitylamino)uracil binding3
Regioni119 – 1215-amino-6-(D-ribitylamino)uracil binding3
Regioni124 – 1251-deoxy-L-glycero-tetrulose 4-phosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the DMRL synthase family.Curated

Phylogenomic databases

InParanoidiQ9UVT8.
KOiK00794.
OrthoDBiEOG092C573E.

Family and domain databases

Gene3Di3.40.50.960. 1 hit.
HAMAPiMF_00178. Lumazine_synth. 1 hit.
InterProiIPR002180. DMRL_synthase.
[Graphical view]
PANTHERiPTHR21058. PTHR21058. 2 hits.
PfamiPF00885. DMRL_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52121. SSF52121. 1 hit.
TIGRFAMsiTIGR00114. lumazine-synth. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UVT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTKGPTPQQ HDGSALRIGI VHARWNETII EPLLAGTKAK LLACGVKESN
60 70 80 90 100
IVVQSVPGSW ELPIAVQRLY SASQLQTPSS GPSLSAGDLL GSSTTDLTAL
110 120 130 140 150
PTTTASSTGP FDALIAIGVL IKGETMHFEY IADSVSHGLM RVQLDTGVPV
160 170 180 190 200
IFGVLTVLTD DQAKARAGVI EGSHNHGEDW GLAAVEMGVR RRDWAAGKTE
Length:200
Mass (Da):21,072
Last modified:May 1, 2000 - v1
Checksum:iE079F049D32271B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148449 mRNA. Translation: AAD55372.1.
CM001231 Genomic DNA. Translation: EHA58265.1.
RefSeqiXP_003710877.1. XM_003710829.1.

Genome annotation databases

EnsemblFungiiMGG_04626T0; MGG_04626T0; MGG_04626.
GeneIDi2677942.
KEGGimgr:MGG_04626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148449 mRNA. Translation: AAD55372.1.
CM001231 Genomic DNA. Translation: EHA58265.1.
RefSeqiXP_003710877.1. XM_003710829.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C41X-ray3.10A/B/C/D/E/F/G/H/I/J1-200[»]
ProteinModelPortaliQ9UVT8.
SMRiQ9UVT8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiMGG_04626T0; MGG_04626T0; MGG_04626.
GeneIDi2677942.
KEGGimgr:MGG_04626.

Organism-specific databases

EuPathDBiFungiDB:MGG_04626.

Phylogenomic databases

InParanoidiQ9UVT8.
KOiK00794.
OrthoDBiEOG092C573E.

Enzyme and pathway databases

UniPathwayiUPA00275; UER00404.
BRENDAi2.5.1.78. 3152.

Miscellaneous databases

EvolutionaryTraceiQ9UVT8.

Family and domain databases

Gene3Di3.40.50.960. 1 hit.
HAMAPiMF_00178. Lumazine_synth. 1 hit.
InterProiIPR002180. DMRL_synthase.
[Graphical view]
PANTHERiPTHR21058. PTHR21058. 2 hits.
PfamiPF00885. DMRL_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52121. SSF52121. 1 hit.
TIGRFAMsiTIGR00114. lumazine-synth. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRIB4_MAGO7
AccessioniPrimary (citable) accession number: Q9UVT8
Secondary accession number(s): A4QVB0, G4MRQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.