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Protein

6,7-dimethyl-8-ribityllumazine synthase

Gene

MGG_04626

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.

Catalytic activityi

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate.

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (MGG_04626)
  2. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei255-amino-6-(D-ribitylamino)uracil1
Active sitei127Proton donorSequence analysis1
Binding sitei1525-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygen1
Binding sitei1661-deoxy-L-glycero-tetrulose 4-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processRiboflavin biosynthesis

Enzyme and pathway databases

BRENDAi2.5.1.78 3152
UniPathwayiUPA00275; UER00404

Names & Taxonomyi

Protein namesi
Recommended name:
6,7-dimethyl-8-ribityllumazine synthase (EC:2.5.1.78)
Short name:
DMRL synthase
Short name:
LS
Short name:
Lumazine synthase
Gene namesi
ORF Names:MGG_04626
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiFungiDB:MGG_04626

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001348551 – 2006,7-dimethyl-8-ribityllumazine synthaseAdd BLAST200

Interactioni

Subunit structurei

Homopentamer.1 Publication

Protein-protein interaction databases

STRINGi318829.MGG_04626T0

Structurei

Secondary structure

1200
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi18 – 23Combined sources6
Helixi27 – 43Combined sources17
Helixi48 – 50Combined sources3
Beta strandi51 – 58Combined sources8
Helixi61 – 75Combined sources15
Beta strandi112 – 121Combined sources10
Helixi127 – 146Combined sources20
Beta strandi150 – 159Combined sources10
Helixi160 – 166Combined sources7
Helixi176 – 195Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C41X-ray3.10A/B/C/D/E/F/G/H/I/J1-200[»]
ProteinModelPortaliQ9UVT8
SMRiQ9UVT8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UVT8

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 615-amino-6-(D-ribitylamino)uracil binding3
Regioni119 – 1215-amino-6-(D-ribitylamino)uracil binding3
Regioni124 – 1251-deoxy-L-glycero-tetrulose 4-phosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the DMRL synthase family.Curated

Phylogenomic databases

InParanoidiQ9UVT8
KOiK00794
OrthoDBiEOG092C573E

Family and domain databases

CDDicd09209 Lumazine_synthase-I, 1 hit
Gene3Di3.40.50.960, 1 hit
HAMAPiMF_00178 Lumazine_synth, 1 hit
InterProiView protein in InterPro
IPR034964 LS
IPR002180 LS/RS
IPR036467 LS/RS_sf
PANTHERiPTHR21058 PTHR21058, 1 hit
PfamiView protein in Pfam
PF00885 DMRL_synthase, 1 hit
SUPFAMiSSF52121 SSF52121, 1 hit
TIGRFAMsiTIGR00114 lumazine-synth, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UVT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTKGPTPQQ HDGSALRIGI VHARWNETII EPLLAGTKAK LLACGVKESN
60 70 80 90 100
IVVQSVPGSW ELPIAVQRLY SASQLQTPSS GPSLSAGDLL GSSTTDLTAL
110 120 130 140 150
PTTTASSTGP FDALIAIGVL IKGETMHFEY IADSVSHGLM RVQLDTGVPV
160 170 180 190 200
IFGVLTVLTD DQAKARAGVI EGSHNHGEDW GLAAVEMGVR RRDWAAGKTE
Length:200
Mass (Da):21,072
Last modified:May 1, 2000 - v1
Checksum:iE079F049D32271B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148449 mRNA Translation: AAD55372.1
CM001231 Genomic DNA Translation: EHA58265.1
RefSeqiXP_003710877.1, XM_003710829.1

Genome annotation databases

EnsemblFungiiMGG_04626T0; MGG_04626T0; MGG_04626
GeneIDi2677942
KEGGimgr:MGG_04626

Similar proteinsi

Entry informationi

Entry nameiRIB4_MAGO7
AccessioniPrimary (citable) accession number: Q9UVT8
Secondary accession number(s): A4QVB0, G4MRQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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