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Protein

6,7-dimethyl-8-ribityllumazine synthase

Gene

MGG_04626

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.

Catalytic activityi

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate.

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (MGG_04626)
  2. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 2515-amino-6-(D-ribitylamino)uracil
Active sitei127 – 1271Proton donorSequence analysis
Binding sitei152 – 15215-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygen
Binding sitei166 – 16611-deoxy-L-glycero-tetrulose 4-phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Riboflavin biosynthesis

Enzyme and pathway databases

BRENDAi2.5.1.78. 3152.
UniPathwayiUPA00275; UER00404.

Names & Taxonomyi

Protein namesi
Recommended name:
6,7-dimethyl-8-ribityllumazine synthase (EC:2.5.1.78)
Short name:
DMRL synthase
Short name:
LS
Short name:
Lumazine synthase
Gene namesi
ORF Names:MGG_04626
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiFungiDB:MGG_04626.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2002006,7-dimethyl-8-ribityllumazine synthasePRO_0000134855Add
BLAST

Interactioni

Subunit structurei

Homopentamer.1 Publication

Structurei

Secondary structure

1
200
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 236Combined sources
Helixi27 – 4317Combined sources
Helixi48 – 503Combined sources
Beta strandi51 – 588Combined sources
Helixi61 – 7515Combined sources
Beta strandi112 – 12110Combined sources
Helixi127 – 14620Combined sources
Beta strandi150 – 15910Combined sources
Helixi160 – 1667Combined sources
Helixi176 – 19520Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C41X-ray3.10A/B/C/D/E/F/G/H/I/J1-200[»]
ProteinModelPortaliQ9UVT8.
SMRiQ9UVT8. Positions 5-199.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UVT8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni59 – 6135-amino-6-(D-ribitylamino)uracil binding
Regioni119 – 12135-amino-6-(D-ribitylamino)uracil binding
Regioni124 – 12521-deoxy-L-glycero-tetrulose 4-phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the DMRL synthase family.Curated

Phylogenomic databases

InParanoidiQ9UVT8.
KOiK00794.
OrthoDBiEOG7Q8D04.

Family and domain databases

Gene3Di3.40.50.960. 1 hit.
HAMAPiMF_00178. Lumazine_synth.
InterProiIPR002180. DMRL_synthase.
[Graphical view]
PANTHERiPTHR21058. PTHR21058. 2 hits.
PfamiPF00885. DMRL_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52121. SSF52121. 1 hit.
TIGRFAMsiTIGR00114. lumazine-synth. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UVT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTKGPTPQQ HDGSALRIGI VHARWNETII EPLLAGTKAK LLACGVKESN
60 70 80 90 100
IVVQSVPGSW ELPIAVQRLY SASQLQTPSS GPSLSAGDLL GSSTTDLTAL
110 120 130 140 150
PTTTASSTGP FDALIAIGVL IKGETMHFEY IADSVSHGLM RVQLDTGVPV
160 170 180 190 200
IFGVLTVLTD DQAKARAGVI EGSHNHGEDW GLAAVEMGVR RRDWAAGKTE
Length:200
Mass (Da):21,072
Last modified:May 1, 2000 - v1
Checksum:iE079F049D32271B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148449 mRNA. Translation: AAD55372.1.
CM001231 Genomic DNA. Translation: EHA58265.1.
RefSeqiXP_003710877.1. XM_003710829.1.

Genome annotation databases

EnsemblFungiiMGG_04626T0; MGG_04626T0; MGG_04626.
GeneIDi2677942.
KEGGimgr:MGG_04626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148449 mRNA. Translation: AAD55372.1.
CM001231 Genomic DNA. Translation: EHA58265.1.
RefSeqiXP_003710877.1. XM_003710829.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C41X-ray3.10A/B/C/D/E/F/G/H/I/J1-200[»]
ProteinModelPortaliQ9UVT8.
SMRiQ9UVT8. Positions 5-199.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiMGG_04626T0; MGG_04626T0; MGG_04626.
GeneIDi2677942.
KEGGimgr:MGG_04626.

Organism-specific databases

EuPathDBiFungiDB:MGG_04626.

Phylogenomic databases

InParanoidiQ9UVT8.
KOiK00794.
OrthoDBiEOG7Q8D04.

Enzyme and pathway databases

UniPathwayiUPA00275; UER00404.
BRENDAi2.5.1.78. 3152.

Miscellaneous databases

EvolutionaryTraceiQ9UVT8.

Family and domain databases

Gene3Di3.40.50.960. 1 hit.
HAMAPiMF_00178. Lumazine_synth.
InterProiIPR002180. DMRL_synthase.
[Graphical view]
PANTHERiPTHR21058. PTHR21058. 2 hits.
PfamiPF00885. DMRL_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52121. SSF52121. 1 hit.
TIGRFAMsiTIGR00114. lumazine-synth. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Crystal structure analysis of a pentameric fungal and an icosahedral plant lumazine synthase reveals the structural basis for differences in assembly."
    Persson K., Schneider G., Jordan D.B., Viitanen P.V., Sandalova T.
    Protein Sci. 8:2355-2365(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG INHIBITOR, SUBUNIT.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.

Entry informationi

Entry nameiRIB4_MAGO7
AccessioniPrimary (citable) accession number: Q9UVT8
Secondary accession number(s): A4QVB0, G4MRQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: May 1, 2000
Last modified: November 11, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.