Q9UVD6 (MEL_TORDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-galactosidase
EC=3.2.1.22

Alternative name(s):
Alpha-D-galactoside galactohydrolase
MELt
Melibiase

Gene names

Name:

MEL

Organism

Torulaspora delbrueckii (Yeast) (Saccharomyces rosei)

Taxonomic identifier

4950 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Torulaspora

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subunit structure	Homotetramer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 474	452	Alpha-galactosidase		PRO_0000001010
Sites
	Active site	150	1	Nucleophile By similarity
	Active site	210	1	Proton donor By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	74	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	206	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
Amino acid modifications
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	414	1	N-linked (GlcNAc...) Potential
	Glycosylation	423	1	N-linked (GlcNAc...) Potential
	Glycosylation	436	1	N-linked (GlcNAc...) Potential
	Glycosylation	455	1	N-linked (GlcNAc...) Potential
	Disulfide bond	43 ↔ 75		By similarity
	Disulfide bond	122 ↔ 152		By similarity
	Disulfide bond	222 ↔ 238		By similarity
	Disulfide bond	224 ↔ 231		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UVD6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 6F058455B3A3D7B1	FASTA	474	52,364
	10 20 30 40 50 60 MINFSLLTSI VLLASKVVGV SPSYNGLGLT PQMGWNNWNT FACNVSEDLL LSTVDRIAAL 70 80 90 100 110 120 GLRDIGYHYV ILDDCWSDGR DSDGMLVPDS TKFPNGMKHV ADYLHGKDFL FGMYSSAGEY 130 140 150 160 170 180 TCAGYAGSLD HEEDDAAFFA KNEVDYLKYD NCYNRGQFGT PETSFNRYRA MSEALNKTER 190 200 210 220 230 240 PIFYSLCNWG QDLTFYWGSG IANSWRISGD ITAEFDRPDS RCPCDGDEYD CPYAGFHCSI 250 260 270 280 290 300 MNILNKAAPM GQNAGVGGWN DLDCLEVGVG NLTDDEEKAH FSMWAIVKSA MVIGADVRNL 310 320 330 340 350 360 KPSSFSIYSQ ASVLAINQDP AGAPAIRVWR RYVPETDQHG QGEVQLWSGP LDNGDRVVAL 370 380 390 400 410 420 LNGGAKERPM VAYLEDIFID SFVGSEELSS TWNVYDLWAN RIDDSTASQI LVGNRTANGL 430 440 450 460 470 LYNATQLSYA DGLKANDTRL FGEKVGTIEP YGLLNVTVPA HGVGLFRLRR ESRK

References

[1]	"Isolation and characterization of MELt gene from Torulaspora delbrueckii IFO 1255." Oda Y., Fukunaga M. Yeast 15:1797-1801(1999) [PubMed: 10590467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NBRC 1255 / IAM 4952.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB027130 Genomic DNA. Translation: BAA86883.1.
3D structure databases
ProteinModelPortal	Q9UVD6.
ModBase	Search...
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR006215. Glyco_hydro_melibiase. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
Pfam	PF02065. Melibiase. 2 hits. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27. PR00748. MELIBIASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MEL_TORDE

Accession

Primary (citable) accession number: Q9UVD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	May 1, 2000
Last modified:	September 21, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program