Reviewed, UniProtKB/Swiss-Prot Q9UVC3 (CP51_CUNEL)

Names and origin

Protein names

Recommended name:
    Lanosterol 14-alpha demethylase
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51
    CYPLI
    Cytochrome P450-LIA1
    Sterol 14-alpha demethylase
    Cytochrome P450-14DM

Gene names

Name:

CYP51

Organism

Cunninghamella elegans

Taxonomic identifier

4853 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Cunninghamellaceae › Cunninghamella

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Lanosterol 14-alpha demethylase		PRO_0000052006
Sites
	Metal binding	458	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UVC3-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5E10C9D415CA15B1	FASTA	512	57,677
	10 20 30 40 50 60 MAIVSQISRF ITFTIISMGY SVLAVGVALT IHILSQLIVP KNPNEPPNVF SLIPVLGNAV 70 80 90 100 110 120 QFGMNPVAFL QECQKKYGDV FTFTMVGKRV TVCLGADGNQ FVFNSKQNLS SAAEAYNHMT 130 140 150 160 170 180 KYVFGPDVVY DAPHAVFMEQ KKFIKAGLNS DCFRQHVPMI VQETEEFFKK FNKPTGFIEA 190 200 210 220 230 240 YETFGSLIIY TASRCLMGKE IRASLDGNVA KLYYDLDQGF KPINFIFPNL PLPSYRRRDV 250 260 270 280 290 300 ACKKMADLYS SIIQRRKDEK DNNNADLLQA LMDATYKDGT HIPDHHIAGM MIAVLFGGQH 310 320 330 340 350 360 TSATTSAWTI LELANRPDII KALREEQIEK LGSLKADLTF DNLKDLPLLE AAIRETLRLH 370 380 390 400 410 420 PPIFQMMRRV VADKIVYEKN GMEIPKGNFI CAAPGVTQVD PTYFNEPTTY NPYRWIEKTD 430 440 450 460 470 480 PVHQLEQGDD ANIDYGFGAV GISSKSPFLP FGAGRHRCIG EQFGYLQLKT VISTFIRTFD 490 500 510 FDLDGKSVPK SDYTSMVVVP EHTAKVRYTW RE

References

[1]	Craft D.L., Loper J.C. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 36112 / DSM 8217.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF046863 Genomic DNA. Translation: AAF20263.1.
3D structure databases
SMR	Q9UVC3. Positions 52-511.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.70. 2421.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_CUNEL

Accession

Primary (citable) accession number: Q9UVC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)