Reviewed, UniProtKB/Swiss-Prot Q9UV50 (HOG1_DEBHA)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase HOG1
      Short name=MAP kinase HOG1
    EC=2.7.11.24

Gene names

Name:	HOG1
Ordered Locus Names:	DEHA2E20944g

Organism

Debaryomyces hansenii (Yeast) (Torulaspora hansenii) [Complete proteome]

Taxonomic identifier

4959 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Ref.1 Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation.
Subcellular location	Cytoplasm. Nucleus. Note: Contrary to other yeasts, the nuclear translocation is delayed under severe osmotic condition although it is activated rapidly. Ref.3
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-174 and Tyr-176, which activates the enzyme By similarity. Phosphorylated in response of osmotic stress, oxidative stress ans UV stress, but not heat stress.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Mitogen-activated protein kinase HOG1		PRO_0000186330
Regions
	Domain	23 – 305	283	Protein kinase
	Nucleotide binding	29 – 37	9	ATP By similarity
	Motif	174 – 176	3	TXY
Sites
	Active site	144	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity
Amino acid modifications
	Modified residue	174	1	Phosphothreonine By similarity
	Modified residue	176	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	272	1	S → T in AAF24231. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9UV50-1 [UniParc]. Last modified December 16, 2008. Version 3. Checksum: 710908EC58AA0828	FASTA	387	44,357
	10 20 30 40 50 60 MVSDGEFTRT QIFGTVFEIT NRYTDLNPVG MGAFGLVCSA IDKLTGQNVA VKKIMKPFST 70 80 90 100 110 120 SVLAKRTYRE LKLLKHLRHE NLITLDDIFL SPLEDIYFVT ELQGTDLHRL LTSRPLEKQF 130 140 150 160 170 180 IQYFTYQILR GLKYVHSAGV IHRDLKPSNI LINENCDLKI CDFGLARIQD PQMTGYVSTR 190 200 210 220 230 240 YYRAPEIMLT WQKYDTEVDL WSVGCILSEM IEGKPLFPGK DHVHQFSIIT ELLGSPPPDV 250 260 270 280 290 300 IETICSENTL RFVQSLPHRD PIPFSERFAQ CSHVEPEAID LLAKMLIFDP KKRISALDAL 310 320 330 340 350 360 SHPYMEPYHD PTDEPVCEVK FDWSFNDADL PVDTWRVMMY SEILDFHQII GAGANGTTQE 370 380 QMAQIQQEGI QAPSSQYQQT NQEQKVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and sequence of the HOG1 homolog from Debaryomyces hansenii by complementation of hog1 Delta strain of Saccharomyces cerevisiae." Bansal P.K., Mondal A.K. Yeast 16:81-88(2000) [PubMed: 10620778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: MTCC 234.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 36239 / CBS 767 / IFO 0083 / IGC 2968 / JCM 1990.
[3]	"Debaryomyces hansenii, a highly osmo-tolerant and halo-tolerant yeast, maintains activated Dhog1p in the cytoplasm during its growth under severe osmotic stress." Sharma P., Meena N., Aggarwal M., Mondal A.K. Curr. Genet. 48:162-170(2005) [PubMed: 16091960] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF185278 Genomic DNA. Translation: AAF24231.2. CR382137 Genomic DNA. Translation: CAG88486.2.
RefSeq	XP_460213.1.
3D structure databases
HSSP	HSSP built from PDB template 1KV1 based on UniProtKB Q16539.
ModBase	Search...
Genome annotation databases
GeneID	2902985.
GenomeReviews	Gene locus DEHA2E20944g in contig CR382137_GR.
KEGG	dha:DEHA0E22110g.
Phylogenomic databases
HOGENOM	Q9UV50.
Enzyme and pathway databases
BRENDA	2.7.11.24. 74267.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_DEBHA

Accession

Primary (citable) accession number: Q9UV50
Secondary accession number(s): Q6BNK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	December 16, 2008
Last modified:	September 22, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)