ID   LCPS_MUCCL              Reviewed;         614 AA.
AC   Q9UUQ6;
DT   19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   13-SEP-2023, entry version 90.
DE   RecName: Full=Bifunctional lycopene cyclase/phytoene synthase {ECO:0000303|PubMed:10951210};
DE   Includes:
DE     RecName: Full=Lycopene beta-cyclase {ECO:0000303|PubMed:10951210};
DE              EC=5.5.1.19 {ECO:0000269|PubMed:10951210};
DE   Includes:
DE     RecName: Full=Phytoene synthase {ECO:0000303|PubMed:10951210};
DE              EC=2.5.1.32 {ECO:0000269|PubMed:10951210};
GN   Name=CARRP {ECO:0000303|PubMed:10951210};
OS   Mucor circinelloides f. lusitanicus (Mucor racemosus var. lusitanicus).
OC   Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC   Mucoromycetes; Mucorales; Mucorineae; Mucoraceae; Mucor.
OX   NCBI_TaxID=29924;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, INDUCTION,
RP   AND VARIANTS LYS-78; ILE-86 AND GLY-409.
RC   STRAIN=ATCC 1216b / BCRC 32522 / CBS 277.49 / NRRL 3631, MS21, MS35,
RC   and MS36;
RX   PubMed=10951210; DOI=10.1046/j.1432-1327.2000.01612.x;
RA   Velayos A., Eslava A.P., Iturriaga E.A.;
RT   "A bifunctional enzyme with lycopene cyclase and phytoene synthase
RT   activities is encoded by the carRP gene of Mucor circinelloides.";
RL   Eur. J. Biochem. 267:5509-5519(2000).
CC   -!- FUNCTION: Bifunctional enzyme that catalyzes the reactions from
CC       prephytoene diphosphate to phytoene and lycopene to beta-carotene via
CC       the intermediate gamma-carotene. {ECO:0000269|PubMed:10951210}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=all-trans-lycopene = gamma-carotene; Xref=Rhea:RHEA:32219,
CC         ChEBI:CHEBI:15948, ChEBI:CHEBI:27740; EC=5.5.1.19;
CC         Evidence={ECO:0000269|PubMed:10951210};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=gamma-carotene = all-trans-beta-carotene;
CC         Xref=Rhea:RHEA:32239, ChEBI:CHEBI:17579, ChEBI:CHEBI:27740;
CC         EC=5.5.1.19; Evidence={ECO:0000269|PubMed:10951210};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC         diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC         Evidence={ECO:0000269|PubMed:10951210};
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.
CC       {ECO:0000269|PubMed:10951210}.
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-
CC       phytoene from geranylgeranyl diphosphate: step 1/1.
CC       {ECO:0000269|PubMed:10951210}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: By blue light. {ECO:0000269|PubMed:10951210}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the lycopene beta-
CC       cyclase family. {ECO:0000305}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the phytoene/squalene
CC       synthase family. {ECO:0000305}.
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DR   EMBL; AJ250827; CAB60272.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9UUQ6; -.
DR   SMR; Q9UUQ6; -.
DR   UniPathway; UPA00799; UER00773.
DR   UniPathway; UPA00802; -.
DR   GO; GO:0016020; C:membrane; NAS:UniProtKB.
DR   GO; GO:0046905; F:15-cis-phytoene synthase activity; IDA:UniProtKB.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016872; F:intramolecular lyase activity; IEA:InterPro.
DR   GO; GO:0045436; F:lycopene beta cyclase activity; IDA:UniProtKB.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR017825; Lycopene_cyclase_dom.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   NCBIfam; TIGR03462; CarR_dom_SF; 2.
DR   PANTHER; PTHR31480; BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE; 1.
DR   PANTHER; PTHR31480:SF2; PHYTOENE SYNTHASE, CHLOROPLASTIC; 1.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR   SUPFAM; SSF48576; Terpenoid synthases; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Isomerase; Membrane; Multifunctional enzyme;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..614
FT                   /note="Bifunctional lycopene cyclase/phytoene synthase"
FT                   /id="PRO_0000067441"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        217..237
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..239
FT                   /note="Lycopene beta-cyclase"
FT                   /evidence="ECO:0000305|PubMed:10951210"
FT   REGION          246..614
FT                   /note="Phytoene synthase"
FT                   /evidence="ECO:0000305|PubMed:10951210"
FT   VARIANT         78
FT                   /note="E -> K (in strain: MS21; loss of lycopene cyclase
FT                   activity)"
FT                   /evidence="ECO:0000269|PubMed:10951210"
FT   VARIANT         86
FT                   /note="T -> I (in strain: MS35; loss of lycopene cyclase
FT                   activity)"
FT                   /evidence="ECO:0000269|PubMed:10951210"
FT   VARIANT         409
FT                   /note="D -> G (in strain: MS36; loss of phytoene cyclase
FT                   activity)"
FT                   /evidence="ECO:0000269|PubMed:10951210"
SQ   SEQUENCE   614 AA;  69841 MW;  599E6BB541087FE5 CRC64;
     MLLTYMEVHL YYTLPVLGVL SWLSRPYYTA TDALKFKFLT LVAFTTASAW DNYIVYHKAW
     SYCPTCVTAV IGYVPLEEYM FFIIMTLLTV AFTNLVMRWH LHSFFIRPET PVMQSVLVRL
     VPITALLITA YKAWHLAVPG KPLFYGSCIL WYACPVLALL WFGAGEYMMR RPLAVLVSIA
     LPTLFLCWVD VVAIGAGTWD ISLATSTGKF VVPHLPVEEF MFFALINTVL VFGTCAIDRT
     MAILHLFKNK SPYQRPYQHS KSFLHQILEM TWAFCLPDQV LHSDTFHDLS VSWDILRKAS
     KSFYTASAVF PGDVRQELGV LYAFCRATDD LCDNEQVPVQ TRKEQLILTH QFVSDLFGQK
     TSAPTAIDWD FYNDQLPASC ISAFKSFTRL RHVLEAGAIK ELLDGYKWDL ERRSIRDQED
     LRYYSACVAS SVGEMCTRII LAHADKPASR QQTQWIIQRA REMGLVLQYT NIARDIVTDS
     EELGRCYLPQ DWLTEKEVAL IQGGLAREIG EERLLSLSHR LIYQADELMV VANKGIDKLP
     SHCQGGVRAA CNVYASIGTK LKSYKHHYPS RAHVGNSKRV EIALLSVYNL YTAPIATSST
     THCRQGKMRN LNTI
//