Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

CARRP

Organism
Mucor circinelloides f. lusitanicus (Mucor racemosus var. lusitanicus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional enzyme that catalyzes the reactions from prephytoene diphosphate to phytoene and lycopene to beta-carotene via the intermediate gamma-carotene.1 Publication

Catalytic activityi

Carotenoid psi-end group = carotenoid beta-end group.
2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathwayi

GO - Molecular functioni

  1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB-EC
  2. intramolecular lyase activity Source: InterPro
  3. lycopene beta cyclase activity Source: UniProtKB
  4. phytoene synthase activity Source: UniProtKB

GO - Biological processi

  1. carotene biosynthetic process Source: GOC
  2. carotenoid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lycopene cyclase/phytoene synthase
Including the following 2 domains:
Lycopene beta-cyclase (EC:5.5.1.19)
Phytoene synthase (EC:2.5.1.32)
Gene namesi
Name:CARRP
OrganismiMucor circinelloides f. lusitanicus (Mucor racemosus var. lusitanicus)
Taxonomic identifieri29924 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesMucorineaeMucoraceaeMucor

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221HelicalSequence AnalysisAdd
BLAST
Transmembranei36 – 5621HelicalSequence AnalysisAdd
BLAST
Transmembranei70 – 9021HelicalSequence AnalysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence AnalysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence AnalysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614Bifunctional lycopene cyclase/phytoene synthasePRO_0000067441Add
BLAST

Expressioni

Inductioni

By blue light.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9UUQ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 239239Lycopene beta-cyclaseAdd
BLAST
Regioni246 – 614369Phytoene synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UUQ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLTYMEVHL YYTLPVLGVL SWLSRPYYTA TDALKFKFLT LVAFTTASAW
60 70 80 90 100
DNYIVYHKAW SYCPTCVTAV IGYVPLEEYM FFIIMTLLTV AFTNLVMRWH
110 120 130 140 150
LHSFFIRPET PVMQSVLVRL VPITALLITA YKAWHLAVPG KPLFYGSCIL
160 170 180 190 200
WYACPVLALL WFGAGEYMMR RPLAVLVSIA LPTLFLCWVD VVAIGAGTWD
210 220 230 240 250
ISLATSTGKF VVPHLPVEEF MFFALINTVL VFGTCAIDRT MAILHLFKNK
260 270 280 290 300
SPYQRPYQHS KSFLHQILEM TWAFCLPDQV LHSDTFHDLS VSWDILRKAS
310 320 330 340 350
KSFYTASAVF PGDVRQELGV LYAFCRATDD LCDNEQVPVQ TRKEQLILTH
360 370 380 390 400
QFVSDLFGQK TSAPTAIDWD FYNDQLPASC ISAFKSFTRL RHVLEAGAIK
410 420 430 440 450
ELLDGYKWDL ERRSIRDQED LRYYSACVAS SVGEMCTRII LAHADKPASR
460 470 480 490 500
QQTQWIIQRA REMGLVLQYT NIARDIVTDS EELGRCYLPQ DWLTEKEVAL
510 520 530 540 550
IQGGLAREIG EERLLSLSHR LIYQADELMV VANKGIDKLP SHCQGGVRAA
560 570 580 590 600
CNVYASIGTK LKSYKHHYPS RAHVGNSKRV EIALLSVYNL YTAPIATSST
610
THCRQGKMRN LNTI
Length:614
Mass (Da):69,841
Last modified:May 1, 2000 - v1
Checksum:i599E6BB541087FE5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti78 – 781E → K in strain: MS21; loss of lycopene cyclase activity. 1 Publication
Natural varianti86 – 861T → I in strain: MS35; loss of lycopene cyclase activity. 1 Publication
Natural varianti409 – 4091D → G in strain: MS36; loss of phytoene cyclase activity. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250827 Genomic DNA. Translation: CAB60272.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ250827 Genomic DNA. Translation: CAB60272.1.

3D structure databases

ProteinModelPortaliQ9UUQ6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A bifunctional enzyme with lycopene cyclase and phytoene synthase activities is encoded by the carRP gene of Mucor circinelloides."
    Velayos A., Eslava A.P., Iturriaga E.A.
    Eur. J. Biochem. 267:5509-5519(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, VARIANTS LYS-78; ILE-86 AND GLY-409.
    Strain: ATCC 1216b / CBS 277.49 / NRRL 3631, MS21, MS35 and MS36.

Entry informationi

Entry nameiLCPS_MUCCL
AccessioniPrimary (citable) accession number: Q9UUQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: May 1, 2000
Last modified: April 1, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.