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Q9UUQ6 (LCPS_MUCCL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

  1. Lycopene beta-cyclase
    EC=5.5.1.19
  2. Phytoene synthase
    EC=2.5.1.32
Gene names
Name:CARRP
OrganismMucor circinelloides f. lusitanicus (Mucor racemosus var. lusitanicus)
Taxonomic identifier29924 [NCBI]
Taxonomic lineageEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesMucorineaeMucoraceaeMucor

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the reactions from prephytoene diphosphate to phytoene and lycopene to beta-carotene via the intermediate gamma-carotene. Ref.1

Catalytic activity

Carotenoid psi-end group = carotenoid beta-end group.

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathway

Carotenoid biosynthesis; beta-carotene biosynthesis. Ref.1

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Induction

By blue light. Ref.1

Sequence similarities

In the N-terminal section; belongs to the lycopene beta-cyclase family.

In the C-terminal section; belongs to the phytoene/squalene synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 614614Bifunctional lycopene cyclase/phytoene synthase
PRO_0000067441

Regions

Transmembrane2 – 2221Helical; Potential
Transmembrane36 – 5621Helical; Potential
Transmembrane70 – 9021Helical; Potential
Transmembrane120 – 14021Helical; Potential
Transmembrane143 – 16321Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane217 – 23721Helical; Potential
Region1 – 239239Lycopene beta-cyclase
Region246 – 614369Phytoene synthase

Natural variations

Natural variant781E → K in strain: MS21; loss of lycopene cyclase activity. Ref.1
Natural variant861T → I in strain: MS35; loss of lycopene cyclase activity. Ref.1
Natural variant4091D → G in strain: MS36; loss of phytoene cyclase activity. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9UUQ6 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 599E6BB541087FE5

FASTA61469,841
        10         20         30         40         50         60 
MLLTYMEVHL YYTLPVLGVL SWLSRPYYTA TDALKFKFLT LVAFTTASAW DNYIVYHKAW 

        70         80         90        100        110        120 
SYCPTCVTAV IGYVPLEEYM FFIIMTLLTV AFTNLVMRWH LHSFFIRPET PVMQSVLVRL 

       130        140        150        160        170        180 
VPITALLITA YKAWHLAVPG KPLFYGSCIL WYACPVLALL WFGAGEYMMR RPLAVLVSIA 

       190        200        210        220        230        240 
LPTLFLCWVD VVAIGAGTWD ISLATSTGKF VVPHLPVEEF MFFALINTVL VFGTCAIDRT 

       250        260        270        280        290        300 
MAILHLFKNK SPYQRPYQHS KSFLHQILEM TWAFCLPDQV LHSDTFHDLS VSWDILRKAS 

       310        320        330        340        350        360 
KSFYTASAVF PGDVRQELGV LYAFCRATDD LCDNEQVPVQ TRKEQLILTH QFVSDLFGQK 

       370        380        390        400        410        420 
TSAPTAIDWD FYNDQLPASC ISAFKSFTRL RHVLEAGAIK ELLDGYKWDL ERRSIRDQED 

       430        440        450        460        470        480 
LRYYSACVAS SVGEMCTRII LAHADKPASR QQTQWIIQRA REMGLVLQYT NIARDIVTDS 

       490        500        510        520        530        540 
EELGRCYLPQ DWLTEKEVAL IQGGLAREIG EERLLSLSHR LIYQADELMV VANKGIDKLP 

       550        560        570        580        590        600 
SHCQGGVRAA CNVYASIGTK LKSYKHHYPS RAHVGNSKRV EIALLSVYNL YTAPIATSST 

       610 
THCRQGKMRN LNTI 

« Hide

References

[1]"A bifunctional enzyme with lycopene cyclase and phytoene synthase activities is encoded by the carRP gene of Mucor circinelloides."
Velayos A., Eslava A.P., Iturriaga E.A.
Eur. J. Biochem. 267:5509-5519(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, VARIANTS LYS-78; ILE-86 AND GLY-409.
Strain: ATCC 1216b / CBS 277.49 / NRRL 3631, MS21, MS35 and MS36.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ250827 Genomic DNA. Translation: CAB60272.1.

3D structure databases

ProteinModelPortalQ9UUQ6.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00799; UER00773.
UPA00802.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
TIGRFAMsTIGR03462. CarR_dom_SF. 2 hits.
PROSITEPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLCPS_MUCCL
AccessionPrimary (citable) accession number: Q9UUQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways