Q9UUQ6 (LCPS_MUCCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

1. Lycopene beta-cyclase
   EC=5.5.1.19
2. Phytoene synthase
   EC=2.5.1.32

Gene names

Name:

CARRP

Organism

Mucor circinelloides f. lusitanicus (Mucor racemosus var. lusitanicus)

Taxonomic identifier

29924 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Fungi incertae sedis › Early diverging fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Mucor › Mucor circinelloides

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the reactions from prephytoene diphosphate to phytoene and lycopene to beta-carotene via the intermediate gamma-carotene. Ref.1
Catalytic activity	Carotenoid psi-end group = carotenoid beta-end group. 2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; beta-carotene biosynthesis. Ref.1 Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Induction	By blue light. Ref.1
Sequence similarities	In the N-terminal section; belongs to the lycopene beta-cyclase family. In the C-terminal section; belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Isomerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC intramolecular lyase activity Inferred from electronic annotation. Source: InterPro lycopene beta cyclase activity Inferred from direct assay Ref.1. Source: UniProtKB phytoene synthase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Bifunctional lycopene cyclase/phytoene synthase		PRO_0000067441
Regions
	Transmembrane	2 – 22	21	Helical; Potential
	Transmembrane	36 – 56	21	Helical; Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Transmembrane	120 – 140	21	Helical; Potential
	Transmembrane	143 – 163	21	Helical; Potential
	Transmembrane	174 – 194	21	Helical; Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Region	1 – 239	239	Lycopene beta-cyclase
	Region	246 – 614	369	Phytoene synthase
Natural variations
	Natural variant	78	1	E → K in strain: MS21; loss of lycopene cyclase activity. Ref.1
	Natural variant	86	1	T → I in strain: MS35; loss of lycopene cyclase activity. Ref.1
	Natural variant	409	1	D → G in strain: MS36; loss of phytoene cyclase activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9UUQ6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 599E6BB541087FE5	FASTA	614	69,841
	10 20 30 40 50 60 MLLTYMEVHL YYTLPVLGVL SWLSRPYYTA TDALKFKFLT LVAFTTASAW DNYIVYHKAW 70 80 90 100 110 120 SYCPTCVTAV IGYVPLEEYM FFIIMTLLTV AFTNLVMRWH LHSFFIRPET PVMQSVLVRL 130 140 150 160 170 180 VPITALLITA YKAWHLAVPG KPLFYGSCIL WYACPVLALL WFGAGEYMMR RPLAVLVSIA 190 200 210 220 230 240 LPTLFLCWVD VVAIGAGTWD ISLATSTGKF VVPHLPVEEF MFFALINTVL VFGTCAIDRT 250 260 270 280 290 300 MAILHLFKNK SPYQRPYQHS KSFLHQILEM TWAFCLPDQV LHSDTFHDLS VSWDILRKAS 310 320 330 340 350 360 KSFYTASAVF PGDVRQELGV LYAFCRATDD LCDNEQVPVQ TRKEQLILTH QFVSDLFGQK 370 380 390 400 410 420 TSAPTAIDWD FYNDQLPASC ISAFKSFTRL RHVLEAGAIK ELLDGYKWDL ERRSIRDQED 430 440 450 460 470 480 LRYYSACVAS SVGEMCTRII LAHADKPASR QQTQWIIQRA REMGLVLQYT NIARDIVTDS 490 500 510 520 530 540 EELGRCYLPQ DWLTEKEVAL IQGGLAREIG EERLLSLSHR LIYQADELMV VANKGIDKLP 550 560 570 580 590 600 SHCQGGVRAA CNVYASIGTK LKSYKHHYPS RAHVGNSKRV EIALLSVYNL YTAPIATSST 610 THCRQGKMRN LNTI

References

[1]

"A bifunctional enzyme with lycopene cyclase and phytoene synthase activities is encoded by the carRP gene of Mucor circinelloides." Velayos A., Eslava A.P., Iturriaga E.A. Eur. J. Biochem. 267:5509-5519(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, VARIANTS LYS-78; ILE-86 AND GLY-409. Strain: ATCC 1216b / CBS 277.49 / NRRL 3631, MS21, MS35 and MS36.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ250827 Genomic DNA. Translation: CAB60272.1.
3D structure databases
ProteinModelPortal	Q9UUQ6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00799; UER00773. UPA00802.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR017825. Lycopene_cyclase_dom. IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR03462. CarR_dom_SF. 2 hits.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. False negative. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCPS_MUCCL

Accession

Primary (citable) accession number: Q9UUQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program