Q9UUK7 (PUR3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylglycinamide formyltransferase
EC=2.1.2.2

Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART

Gene names

Name:	ade5
Synonyms:	ade8
ORF Names:	SPCC569.08c, SPCC24E4.01

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Sequence similarities	Belongs to the GART family.
Sequence caution	The sequence BAA87143.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe adenine biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: GeneDB_Spombe nucleus Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: InterPro phosphoribosylglycinamide formyltransferase activity Inferred from sequence or structural similarity. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Phosphoribosylglycinamide formyltransferase		PRO_0000074953
Regions
	Region	13 – 15	3	5'-phosphoribosylglycinamide binding By similarity
	Region	100 – 103	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	122	1	Proton donor By similarity
	Binding site	120	1	10-formyltetrahydrofolate By similarity
	Binding site	162	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	191	1	5'-phosphoribosylglycinamide By similarity
	Site	162	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9UUK7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 0ED9C5257488CA36	FASTA	207	22,252
	10 20 30 40 50 60 MVASLVVLIS GSGSNLQAII DATLNGVLKG EAAVTHVLSN RKNAYGLERA AKAGIPTSLH 70 80 90 100 110 120 TLLPYKKEYG PEIGRKKYDA ELAEKIIKLQ PSLVVCAGWM HILSPEVLIP LETNKIGIIN 130 140 150 160 170 180 LHPALPGAFN GIHAIERAFE AAQQGKITHT GAMVHWVIAA VDEGKPIIVQ EVPILSTDSI 190 200 EALEEKIHAA EHVILVQAIH QIITDNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The fission yeast cDNA complementing the budding yeast ade8 mutation: cloning, sequencing, and application to genetic analysis." Toh-e A., Oguchi T. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: TP4-1D.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library." Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y. Genes Cells 5:169-190(2000) [PubMed: 10759889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-147. Strain: ATCC 38364 / 968.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF171879 mRNA. Translation: AAD46927.1. CU329672 Genomic DNA. Translation: CAB42069.1. AB027839 Genomic DNA. Translation: BAA87143.1. Different initiation.
PIR	T51296.
RefSeq	XP_001713172.1. XM_001713120.1.
3D structure databases
ProteinModelPortal	Q9UUK7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UUK7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPCC569.08c.1; SPCC569.08c.1:pep; SPCC569.08c.
GeneID	2538712.
GenomeReviews	Gene locus ade5 in contig CU329672_GR.
KEGG	spo:SPCC569.08c.
NMPDR	fig|4896.1.peg.903.
Organism-specific databases
GeneDB_Spombe	SPCC569.08c.
Phylogenomic databases
eggNOG	fuNOG10564.
GeneTree	EFGT00050000003453.
HOGENOM	HBG649382.
OMA	SRIINTH.
OrthoDB	EOG46DQC3.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000011-MONOMER.
Gene expression databases
ArrayExpress	Q9UUK7.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K00601.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_SCHPO

Accession

Primary (citable) accession number: Q9UUK7
Secondary accession number(s): Q9UU36, Q9Y7S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program