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Protein

Actin-related protein 2

Gene

arp2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament (By similarity). During cytokinesis it colocalizes to the cortical actin patches until spetation is complete. Has a role in the mobility of these patches. Essential for viability.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi156 – 1583ATPBy similarity
Nucleotide bindingi210 – 2145ATPBy similarity
Nucleotide bindingi301 – 3066ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • actin cortical patch assembly Source: PomBase
  • Arp2/3 complex-mediated actin nucleation Source: PomBase
  • regulation of endocytosis Source: PomBase
Complete GO annotation...

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 2
Alternative name(s):
Actin-like protein 2
Gene namesi
Name:arp2
ORF Names:SPAC11H11.06, SPAC22F8.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC11H11.06.
PomBaseiSPAC11H11.06. arp2.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: PomBase
  • Arp2/3 protein complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Actin-related protein 2PRO_0000089076Add
BLAST

Proteomic databases

MaxQBiQ9UUJ1.

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of arp2, act2, arc1/p41-ARC, arc2/p34-ARC, arc3/p21-ARC, arc4/p20-ARC and arc5/p16-ARC.1 Publication

Protein-protein interaction databases

BioGridi858103. 16 interactions.
IntActiQ9UUJ1. 8 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9UUJ1.
SMRiQ9UUJ1. Positions 136-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP2 subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000233340.
InParanoidiQ9UUJ1.
KOiK17260.
OMAiSTYIAIQ.
OrthoDBiEOG7NPG3Q.
PhylomeDBiQ9UUJ1.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF37. PTHR11937:SF37. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UUJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESAPIVLDN GTGFVKVGYA KDNFPRFQFP SIVGRPILRA EEKTGNVQIK
60 70 80 90 100
DVMVGDEAEA VRSLLQVKYP MENGIIRDFE EMNQLWDYTF FEKLKIDPRG
110 120 130 140 150
RKILLTEPPM NPVANREKMC ETMFERYGFG GVYVAIQAVL SLYAQGLSSG
160 170 180 190 200
VVVDSGDGVT HIVPVYESVV LNHLVGRLDV AGRDATRYLI SLLLRKGYAF
210 220 230 240 250
NRTADFETVR EMKEKLCYVS YDLELDHKLS EETTVLMRNY TLPDGRVIKV
260 270 280 290 300
GSERYECPEC LFQPHLVGSE QPGLSEFIFD TIQAADVDIR KYLYRAIVLS
310 320 330 340 350
GGSSMYAGLP SRLEKEIKQL WFERVLHGDP ARLPNFKVKI EDAPRRRHAV
360 370 380 390
FIGGAVLADI MAQNDHMWVS KAEWEEYGVR ALDKLGPRTT
Length:390
Mass (Da):44,231
Last modified:May 1, 2000 - v1
Checksum:iD1439F6B49E4E306
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti358 – 3581A → V in AAF21808 (PubMed:10588653).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095900 Genomic DNA. Translation: AAF21808.1.
CU329670 Genomic DNA. Translation: CAB59802.1.
PIRiT38191.
RefSeqiXP_001713111.1. XM_001713059.2.

Genome annotation databases

EnsemblFungiiSPAC11H11.06.1; SPAC11H11.06.1:pep; SPAC11H11.06.
GeneIDi5802965.
KEGGispo:SPAC11H11.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095900 Genomic DNA. Translation: AAF21808.1.
CU329670 Genomic DNA. Translation: CAB59802.1.
PIRiT38191.
RefSeqiXP_001713111.1. XM_001713059.2.

3D structure databases

ProteinModelPortaliQ9UUJ1.
SMRiQ9UUJ1. Positions 136-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi858103. 16 interactions.
IntActiQ9UUJ1. 8 interactions.

Proteomic databases

MaxQBiQ9UUJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC11H11.06.1; SPAC11H11.06.1:pep; SPAC11H11.06.
GeneIDi5802965.
KEGGispo:SPAC11H11.06.

Organism-specific databases

EuPathDBiFungiDB:SPAC11H11.06.
PomBaseiSPAC11H11.06. arp2.

Phylogenomic databases

HOGENOMiHOG000233340.
InParanoidiQ9UUJ1.
KOiK17260.
OMAiSTYIAIQ.
OrthoDBiEOG7NPG3Q.
PhylomeDBiQ9UUJ1.

Miscellaneous databases

NextBioi20892273.
PROiQ9UUJ1.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF37. PTHR11937:SF37. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mutant of arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast."
    Morrell J.L., Morphew M., Gould K.L.
    Mol. Biol. Cell 10:4201-4215(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE ARP2/3 COMPLEX.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiARP2_SCHPO
AccessioniPrimary (citable) accession number: Q9UUJ1
Secondary accession number(s): Q9US62, Q9UTP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 2000
Last modified: November 11, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.