Q9UUI7 (TPSY_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 100 kDa subunit
EC=2.4.1.15

Alternative name(s):
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase

Gene names

ORF Names:

SPAC22F8.05

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	891 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Sequence similarities	In the N-terminal section; belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to stress Inferred from expression pattern PubMed 12529438. Source: PomBase trehalose biosynthetic process Inferred from sequence orthology. Source: PomBase
Cellular_component	alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) Inferred from sequence orthology. Source: PomBase cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 891	891	Putative alpha,alpha-trehalose-phosphate synthase [UDP-forming] 100 kDa subunit		PRO_0000122513
Regions
	Region	132 – 613	482	Glycosyltransferase
Amino acid modifications
	Modified residue	88	1	Phosphothreonine Ref.2
	Modified residue	108	1	Phosphoserine Ref.2
	Modified residue	109	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9UUI7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7F74688CCBFC45B9	FASTA	891	100,661
	10 20 30 40 50 60 MGRQFICSIY LPYTINFHLD ELEGNHESHP AITHQEKVTQ THRDSVKIDD ILTRLSISKS 70 80 90 100 110 120 ESGQATPVLT PQLEGMNDYF SLGPSKRTGG SMTPGLGAMS PIPGSGRSSP LYTQPRSRAT 130 140 150 160 170 180 SPSRVRQADR FAAPGIGAGA LPIRRKRRDS LAKSVALFES ARWSVERGVV GNSGLFHAVD 190 200 210 220 230 240 AAVRDHGLQN PLWVGLLGMP TESLSEKTKN AISGALLVKH QSLVVYTSDS NFEGHYNHYC 250 260 270 280 290 300 RKILWPSLHY QHNEIFSFFH EESNWDDYVA VNRAFADALI KNYKTGDTIW VNDYHLLLVP 310 320 330 340 350 360 NMVRERIPSA IIGLFIHVSF PSSEVFRCFA RRKELLQGML GSNLIGFQTE EYKRHFLQSC 370 380 390 400 410 420 SRVLYAESTF DRILLDDRYI DVYAHPIGAD PVLVDKWLEN PETLEVKEVL EKRYANLNIF 430 440 450 460 470 480 VGCDKMDPIR GIREKLLAFE QFLYDNPEYQ KNTILIQTST FTEEQKEYGV AISDIVTRIN 490 500 510 520 530 540 SAFGDFSLDH LPVTILSSDL SYPQYLALLS VADAFIVTSL REGMSLTCHE FILTQRQKKS 550 560 570 580 590 600 PLIVSEFIGC ASMFSNGAFI VNPWSTLELS LSMKKALTLS TNERNQRYSN CLDVVLTHSA 610 620 630 640 650 660 SNWVTGFETK LKKSWTSQQK RDFSRLPRFT LNFIGNRYDH AKKRLLILNF DGNAVTWEGR 670 680 690 700 710 720 HEFVDFHYGY MVSILSKLIA DDRNIVYIAS CLEEDELESL FMHVPGVGLI AENGCYVLPH 730 740 750 760 770 780 YAENVHQSWI RLYKKQQMDW REPLHDIIQY YSERTPGSSL IDHGFAMEFN YVKAENRENG 790 800 810 820 830 840 LRSAGELASS INETQHGCRA VPLDGRVLCE PTTISKATAA NYIMTHLIKN PEELDLILVA 850 860 870 880 890 GNNRTDESVF AWANKSKVSS FTVSMGVGNT EAKAYTDGIP SFFNVLNSLC A

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-88; SER-108 AND SER-109, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB52715.1.
PIR	T38195.
RefSeq	NP_594728.1. NM_001020156.2.
3D structure databases
ProteinModelPortal	Q9UUI7.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC22F8.05-1.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC22F8.05.1; SPAC22F8.05.1:pep; SPAC22F8.05.
GeneID	2541903.
KEGG	spo:SPAC22F8.05.
Organism-specific databases
PomBase	SPAC22F8.05.
Phylogenomic databases
eggNOG	COG0380.
HOGENOM	HOG000179930.
KO	K16055.
OMA	TCHEFIL.
OrthoDB	EOG4JQ762.
Family and domain databases
Gene3D	3.40.50.1000. 2 hits.
InterPro	IPR001830. Glyco_trans_20. IPR023214. HAD-like_dom. IPR003337. Trehalose_PPase. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. PF02358. Trehalose_PPase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
ProtoNet	Search...
Other
NextBio	20802990.

Entry information

Entry name

TPSY_SCHPO

Accession

Primary (citable) accession number: Q9UUI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program