Reviewed, UniProtKB/Swiss-Prot Q9UUF2 (SYMC_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable methionyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

ORF Names:

SPBC17A3.04c, pi042

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	782 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence BAA21422.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred by curator. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 782	782	Probable methionyl-tRNA synthetase, cytoplasmic		PRO_0000139268
Regions
	Motif	231 – 241	11	"HIGH" region

Sequences

	Sequence		Length	Mass (Da)
	Q9UUF2-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: DAB49CB4758748C2	FASTA	782	88,881
	10 20 30 40 50 60 MATYKVQIPA SFKTSYSFAE SLKVSIAISA FSVKVPCEGA ANCNSVRLVN SKEPEKYVSD 70 80 90 100 110 120 ANAIVSFLYW KQEEDLFNSF ISSKLSILDW EALQFTPKAY TAKTKEDFAY LLSQLETIFK 130 140 150 160 170 180 ENEILNEFTP VEVALASDIY FCVLNGAPVR EYPLLSAWYL KIEKQKPFVQ ALKLTFEKTL 190 200 210 220 230 240 GQPAVTSTEK IPVSETTRNV NSQHLMRERV PGEKILPKSN ERNILITSAL PYVNNVPHLG 250 260 270 280 290 300 NIVGSTLSAD VFARYHRARN HNTLYICGTD EYGTATETKA LEEGVSPKEL CDKYHALHKE 310 320 330 340 350 360 VYDWFEIDFD HFGRTTTPKQ TGIAQHIFTK LYNNDYMAID TMTQLYCEVH QGYLADRYVE 370 380 390 400 410 420 GTCPKCGYDD ARGDQCDGCG GLLNAFELID PKCKLDRATP VKRETKHVFL SLDKLQPAVE 430 440 450 460 470 480 SWAMQSAVEG KWSNNGRSIT ESWLKEGLRP RCITRDLKWG TPVPLEEFKG KVLYVWFDAT 490 500 510 520 530 540 IGYISITANY TDEWEKWWRN PEQVKLYQFM GKDNVPFHTV IFPSSLLGTG EKWTMLHHIN 550 560 570 580 590 600 TTDYLNYETG KFSKSRGVGV FGNTAQDIGL SPSVWRYYLL SSRPETSDTM FTWKEFITRH 610 620 630 640 650 660 NSELLANLGN FVNRTLKFTT AKYNGLVPHY LTDPSVGAGK LKADFVKDVN ALLAKYNAAL 670 680 690 700 710 720 EASKLREGLR LAMEISARGN QYLQDNRIDN KCYLYERQKC ADAIGYALNL IYLLAAIFYP 730 740 750 760 770 780 YMPSTSTSIY KQLNAPAAAI PDTWELCLLP GHRIGEPEYL FTRIDESMEE EWRSKYGGNG SN

References

[1]

"A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment." Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M. Yeast 16:71-80(2000) [PubMed: 10620777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB004537 Genomic DNA. Translation: BAA21422.1. Sequence problems. CU329671 Genomic DNA. Translation: CAB51763.1.
PIR	T39696.
RefSeq	NP_595586.1.
3D structure databases
HSSP	HSSP built from PDB template 1MEA based on UniProtKB P00959.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UUF2.
Genome annotation databases
GeneID	2540052.
KEGG	spo:SPBC17A3.04c.
NMPDR	fig|4896.1.peg.1452.
Organism-specific databases
GeneDB_Spombe	SPBC17A3.04c.
Phylogenomic databases
OMA	YINGIKH.
Enzyme and pathway databases
BRENDA	6.1.1.10. 653.
Gene expression databases
ArrayExpress	Q9UUF2.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYMC_SCHPO

Accession

Primary (citable) accession number: Q9UUF2
Secondary accession number(s): O13634

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)