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Protein

Pyruvate carboxylase

Gene

pyr1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.By similarity

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei150ATPBy similarity1
Binding sitei234ATPBy similarity1
Binding sitei269ATPBy similarity1
Active sitei326By similarity1
Metal bindingi579Divalent metal cationBy similarity1
Binding sitei651SubstrateBy similarity1
Metal bindingi747Divalent metal cation; via carbamate groupBy similarity1
Metal bindingi777Divalent metal cationBy similarity1
Metal bindingi779Divalent metal cationBy similarity1
Binding sitei912SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processGluconeogenesis
LigandATP-binding, Biotin, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-196780 Biotin transport and metabolism
R-SPO-70263 Gluconeogenesis
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylase (EC:6.4.1.1)
Alternative name(s):
Pyruvic carboxylase
Short name:
PCB
Gene namesi
Name:pyr1
ORF Names:SPBC17G9.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC17G9.11c
PomBaseiSPBC17G9.11c pyr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001468231 – 1185Pyruvate carboxylaseAdd BLAST1185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei747N6-carboxylysineBy similarity1
Modified residuei1149N6-biotinyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

MaxQBiQ9UUE1
PaxDbiQ9UUE1
PRIDEiQ9UUE1

PTM databases

iPTMnetiQ9UUE1

Interactioni

Protein-protein interaction databases

BioGridi276216, 1 interactor
STRINGi4896.SPBC17G9.11c.1

Structurei

3D structure databases

ProteinModelPortaliQ9UUE1
SMRiQ9UUE1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 484Biotin carboxylationAdd BLAST453
Domaini154 – 351ATP-graspPROSITE-ProRule annotationAdd BLAST198
Domaini570 – 838Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST269
Domaini1108 – 1183Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni578 – 582Substrate bindingBy similarity5

Phylogenomic databases

HOGENOMiHOG000282801
InParanoidiQ9UUE1
KOiK01958
OMAiGQPHGGF
OrthoDBiEOG092C0BFY
PhylomeDBiQ9UUE1

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR001882 Biotin_BS
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR003379 Carboxylase_cons_dom
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR000891 PYR_CT
IPR005930 Pyruv_COase
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
PF00682 HMGL-like, 1 hit
PF02436 PYC_OADA, 1 hit
PIRSFiPIRSF001594 Pyruv_carbox, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01235 pyruv_carbox, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00867 CPSASE_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UUE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKYDALLH NQSTNTNPFS KLQDRSSLLG EKFTKVLVAN RSEIAIRVFR
60 70 80 90 100
TAHELSMHTV AIYSYEDRLS MHRQKADESY PIGKVGQYSP VGAYLAIDEI
110 120 130 140 150
VSIAKRTGAN LVHPGYGFLS ENAEFARKVN EAGMQFVGPS PEVIDSLGDK
160 170 180 190 200
TKARAIAIRC GVPVVPGTPG PVEHYEEAEA FVKEYGLPVI IKAAMGGGGR
210 220 230 240 250
GMRVVRSADT LKESFERARS EALASFGDGT VFIERFLDKP KHIEIQLMAD
260 270 280 290 300
KAGNVIHLHE RDCSVQRRHQ KVVEIAPAKD LDPKIRQALY DDAIKIAKEV
310 320 330 340 350
KYCNAGTAEF LLDQKGRHYF IEINPRIQVE HTITEEITGV DIVSAQLHVA
360 370 380 390 400
AGFTLPEIGL TQDKISTRGF AIQCRVTTED PNNGFAPDIG KIEVYRSAGG
410 420 430 440 450
NGVRLDGANG FAGSVITPHY DSMLVKCTCH DATYEYTRRK MIRSLIEFRV
460 470 480 490 500
RGVKTNIPFV LRLLMHDTFI QGNCWTTFID DTPELFQLYR SRNRAQKLLA
510 520 530 540 550
YLGDLAVNGS SIKGQNGEPA LKSEIVMPVL LDSTGNQIDV SHPSEKGWRK
560 570 580 590 600
LLLDNGPAAF AKAVRNHKRG LIMDTTWRDA HQSLLATRVR TIDLVNIAPY
610 620 630 640 650
TSHALASAYS LEMWGGATFD VSMRFLHECP WDRLRRLRKL VPNIPFQMLL
660 670 680 690 700
RGANGLCYSS LPDNVIYFFC EQAKKNGIDI FRVFDALNDV NNLSLGIDAA
710 720 730 740 750
KRAGGVVEAT MCYSGDMLNP KKKYNLDYYV NLVDKMVEMG IHILGIKDMA
760 770 780 790 800
GVMKPKAARL LISAIREKHP ELPIHVHTHD SAGTAVASMA AALEAGADVV
810 820 830 840 850
DVATDSMSGL TSQPSFGAVL ASVDGTDKQL EFDNNQLREI DSYWAQMRLL
860 870 880 890 900
YSPFESEIKG TDSDVYNHEI PGGQLTNLKF QATSLGLGTQ WAETKKAYIE
910 920 930 940 950
ANKLLGDIIK VTPTSKVVGD LAQFMVQNKL SAEDVENRAT TLDFPASVLD
960 970 980 990 1000
FFQGLMGQPY GGFPEPLRTN VLKGRRQPLT DRPGKFLPAA DFDAIRKLLS
1010 1020 1030 1040 1050
EKFGVSSDCD IAAYTQFPGV FEEYRQFVDR YGDLTTVPTK FFLSRPEMNE
1060 1070 1080 1090 1100
EMHVEIDQGK TLIVKFVALG PLNPRTGQRE VYFELNGENR HVTVEDKKAA
1110 1120 1130 1140 1150
IETVTRPRAD PGNPGHVAAP MSGTIVEIRV KEGAKVKKGD IIAVLSAMKM
1160 1170 1180
EIVISAPHSG VLKSLAVVQG DSVNGGDLCA VLEHE
Length:1,185
Mass (Da):130,861
Last modified:May 1, 2000 - v1
Checksum:iD39706DCD83915D9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68R → I in BAA11239 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78170 Genomic DNA Translation: BAA11239.1
CU329671 Genomic DNA Translation: CAB52809.1
PIRiT39734
RefSeqiNP_595900.1, NM_001021807.2

Genome annotation databases

EnsemblFungiiSPBC17G9.11c.1; SPBC17G9.11c.1:pep; SPBC17G9.11c
GeneIDi2539661
KEGGispo:SPBC17G9.11c

Similar proteinsi

Entry informationi

Entry nameiPYC_SCHPO
AccessioniPrimary (citable) accession number: Q9UUE1
Secondary accession number(s): P78822
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

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