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Protein

Cytoplasmic tRNA 2-thiolation protein 2

Gene

ctu2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with ctu1 that ligates sulfur from thiocarboxylated urm1 onto the uridine of tRNAs at wobble position. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.UniRule annotation1 Publication

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

  • protein urmylation Source: PomBase
  • tRNA wobble position uridine thiolation Source: PomBase
Complete GO annotation...

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

UniPathwayiUPA00988.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tRNA 2-thiolation protein 2UniRule annotation
Gene namesi
Name:ctu2
Synonyms:ncs2
ORF Names:SPBC19C2.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC19C2.13c.
PomBaseiSPBC19C2.13c. ctu2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Cytoplasmic tRNA 2-thiolation protein 2PRO_0000359408Add
BLAST

Proteomic databases

MaxQBiQ9UUC7.

Interactioni

Subunit structurei

Interacts with ctu1.1 Publication

Protein-protein interaction databases

BioGridi277412. 24 interactions.
DIPiDIP-29901N.
MINTiMINT-4712072.

Structurei

3D structure databases

ProteinModelPortaliQ9UUC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTU2/NCS2 family.UniRule annotation

Phylogenomic databases

InParanoidiQ9UUC7.
KOiK14169.
OMAiEAICTIC.
OrthoDBiEOG7VB2RN.
PhylomeDBiQ9UUC7.

Family and domain databases

HAMAPiMF_03054. CTU2.
InterProiIPR019407. CTU2.
[Graphical view]
PfamiPF10288. CTU2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UUC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNTALDNSA DSKCSKCDNK ATVLTKSDAV CDSCFVRRIE NKIRRQFELV
60 70 80 90 100
RPNLQGRKSK RAMLAISGGI SSMAMLETAN YLSKYRDDNY RPMFDELLAV
110 120 130 140 150
HFQWGTDSAV AKTIEESISK NYPKCPFKVI GEAELLNRTI ATDSRGNIEI
160 170 180 190 200
NADNEKFNPE VISSLASRQD LLYRIRDKLL VSYARKANCD TIVFGDSGTT
210 220 230 240 250
IAARVLELVA EGRGFAIPWY TSVCSKLPNC DTFLLRPLRE VLSSDLKSYM
260 270 280 290 300
NIKGLAFCDS LIEARPNTIH GVTESYFSSL NDTFPSLVST VVKMSSKLHV
310 320 330 340 350
PSTEAICTIC NLPMQEDAET WLQKTTVEHP DSVEGIKNQN VCYGCSVSLK
360
SLKGTLHIPD IEKEGI
Length:366
Mass (Da):40,742
Last modified:May 1, 2000 - v1
Checksum:i7D59F90208A79E09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB52040.1.
PIRiT39804.
RefSeqiNP_595698.1. NM_001021595.2.

Genome annotation databases

EnsemblFungiiSPBC19C2.13c.1; SPBC19C2.13c.1:pep; SPBC19C2.13c.
GeneIDi2540896.
KEGGispo:SPBC19C2.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB52040.1.
PIRiT39804.
RefSeqiNP_595698.1. NM_001021595.2.

3D structure databases

ProteinModelPortaliQ9UUC7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277412. 24 interactions.
DIPiDIP-29901N.
MINTiMINT-4712072.

Proteomic databases

MaxQBiQ9UUC7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC19C2.13c.1; SPBC19C2.13c.1:pep; SPBC19C2.13c.
GeneIDi2540896.
KEGGispo:SPBC19C2.13c.

Organism-specific databases

EuPathDBiFungiDB:SPBC19C2.13c.
PomBaseiSPBC19C2.13c. ctu2.

Phylogenomic databases

InParanoidiQ9UUC7.
KOiK14169.
OMAiEAICTIC.
OrthoDBiEOG7VB2RN.
PhylomeDBiQ9UUC7.

Enzyme and pathway databases

UniPathwayiUPA00988.

Miscellaneous databases

NextBioi20802011.
PROiQ9UUC7.

Family and domain databases

HAMAPiMF_03054. CTU2.
InterProiIPR019407. CTU2.
[Graphical view]
PfamiPF10288. CTU2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  3. "The conserved wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity."
    Dewez M., Bauer F., Dieu M., Raes M., Vandenhaute J., Hermand D.
    Proc. Natl. Acad. Sci. U.S.A. 105:5459-5464(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CTU1.

Entry informationi

Entry nameiCTU2_SCHPO
AccessioniPrimary (citable) accession number: Q9UUC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: May 1, 2000
Last modified: April 13, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.