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Q9UUB6

- UBLH2_SCHPO

UniProt

Q9UUB6 - UBLH2_SCHPO

Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

uch2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei83 – 831NucleophileBy similarity
    Active sitei159 – 1591Proton donorBy similarity
    Sitei174 – 1741Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. omega peptidase activity Source: InterPro
    2. ubiquitin-specific protease activity Source: PomBase

    GO - Biological processi

    1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: PomBase
    2. protein deubiquitination Source: PomBase
    3. protein deubiquitination involved in ubiquitin-dependent protein catabolic process Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.009.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
    Gene namesi
    Name:uch2
    ORF Names:SPBC409.06
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC409.06.

    Subcellular locationi

    Nucleus 2 Publications

    GO - Cellular componenti

    1. nuclear periphery Source: PomBase
    2. nucleus Source: PomBase
    3. proteasome complex Source: PomBase

    Keywords - Cellular componenti

    Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 300300Ubiquitin carboxyl-terminal hydrolase 2PRO_0000234561Add
    BLAST

    Proteomic databases

    MaxQBiQ9UUB6.
    PaxDbiQ9UUB6.

    Interactioni

    Subunit structurei

    Component of the 26S proteasome. Interacts with rpn10.1 Publication

    Protein-protein interaction databases

    BioGridi277472. 63 interactions.
    IntActiQ9UUB6. 1 interaction.
    MINTiMINT-4711794.
    STRINGi4896.SPBC409.06-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9UUB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG321645.
    HOGENOMiHOG000203918.
    KOiK05610.
    OMAiERWAFEN.
    OrthoDBiEOG7FBRWB.
    PhylomeDBiQ9UUB6.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    IPR017390. Ubiquitinyl_hydrolase_UCH37.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
    PRINTSiPR00707. UBCTHYDRLASE.

    Sequencei

    Sequence statusi: Complete.

    Q9UUB6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSWTTIESDA GVFTDLIENL GVKDVEVDEL YSLDVDSLRQ FPDIYGIIFL    50
    FKWNSKVDKP DGTMDYDSMD NIFFAKQVIN NACATQALLS VLLNHSDEID 100
    LGTTLSEFKD FSKTLPPELK GEALGNSEHI RCCHNSFARS DPFISEEVRA 150
    ATDEDEVYHF IAYTNINNVF YELDGLQAAP INHGSCTKEE FAEKAVSVIQ 200
    ARIANYDPAE IRFNLMVICK DKKASLLTRE DLTDEEKAAS IAVEDEKRLR 250
    WKRENQLRRH NFVGLFVELS KLLVKDRIDK NTWNSTLETA KAKYASQKRP 300
    Length:300
    Mass (Da):34,196
    Last modified:May 1, 2000 - v1
    Checksum:i93B13076EF2EDB80
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAB52608.1.
    PIRiT40434.
    RefSeqiNP_595456.1. NM_001021366.2.

    Genome annotation databases

    EnsemblFungiiSPBC409.06.1; SPBC409.06.1:pep; SPBC409.06.
    GeneIDi2540956.
    KEGGispo:SPBC409.06.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAB52608.1 .
    PIRi T40434.
    RefSeqi NP_595456.1. NM_001021366.2.

    3D structure databases

    ProteinModelPortali Q9UUB6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277472. 63 interactions.
    IntActi Q9UUB6. 1 interaction.
    MINTi MINT-4711794.
    STRINGi 4896.SPBC409.06-1.

    Protein family/group databases

    MEROPSi C12.009.

    Proteomic databases

    MaxQBi Q9UUB6.
    PaxDbi Q9UUB6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC409.06.1 ; SPBC409.06.1:pep ; SPBC409.06 .
    GeneIDi 2540956.
    KEGGi spo:SPBC409.06.

    Organism-specific databases

    PomBasei SPBC409.06.

    Phylogenomic databases

    eggNOGi NOG321645.
    HOGENOMi HOG000203918.
    KOi K05610.
    OMAi ERWAFEN.
    OrthoDBi EOG7FBRWB.
    PhylomeDBi Q9UUB6.

    Miscellaneous databases

    NextBioi 20802071.
    PROi Q9UUB6.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    IPR017390. Ubiquitinyl_hydrolase_UCH37.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
    PRINTSi PR00707. UBCTHYDRLASE.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast."
      Stone M., Hartmann-Petersen R., Seeger M., Bech-Otschir D., Wallace M., Gordon C.
      J. Mol. Biol. 344:697-706(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH RPN10, SUBCELLULAR LOCATION.
    3. "Identification of a 26S proteasome-associated UCH in fission yeast."
      Li T., Naqvi N.I., Yang H., Teo T.S.
      Biochem. Biophys. Res. Commun. 272:270-275(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiUBLH2_SCHPO
    AccessioniPrimary (citable) accession number: Q9UUB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3