Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

uch2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831NucleophileBy similarity
Active sitei159 – 1591Proton donorBy similarity
Sitei174 – 1741Important for enzyme activityBy similarity

GO - Molecular functioni

  • omega peptidase activity Source: InterPro
  • ubiquitin-specific protease activity Source: PomBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Gene namesi
Name:uch2
ORF Names:SPBC409.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC409.06.
PomBaseiSPBC409.06. uch2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nuclear periphery Source: PomBase
  • nucleus Source: PomBase
  • proteasome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Ubiquitin carboxyl-terminal hydrolase 2PRO_0000234561Add
BLAST

Proteomic databases

MaxQBiQ9UUB6.
PaxDbiQ9UUB6.

Interactioni

Subunit structurei

Component of the 26S proteasome. Interacts with rpn10.1 Publication

Protein-protein interaction databases

BioGridi277472. 63 interactions.
IntActiQ9UUB6. 1 interaction.
MINTiMINT-4711794.
STRINGi4896.SPBC409.06.1.

Structurei

3D structure databases

ProteinModelPortaliQ9UUB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiNOG321645.
HOGENOMiHOG000203918.
InParanoidiQ9UUB6.
KOiK05610.
OMAiLIREVHN.
OrthoDBiEOG7FBRWB.
PhylomeDBiQ9UUB6.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.

Sequencei

Sequence statusi: Complete.

Q9UUB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWTTIESDA GVFTDLIENL GVKDVEVDEL YSLDVDSLRQ FPDIYGIIFL
60 70 80 90 100
FKWNSKVDKP DGTMDYDSMD NIFFAKQVIN NACATQALLS VLLNHSDEID
110 120 130 140 150
LGTTLSEFKD FSKTLPPELK GEALGNSEHI RCCHNSFARS DPFISEEVRA
160 170 180 190 200
ATDEDEVYHF IAYTNINNVF YELDGLQAAP INHGSCTKEE FAEKAVSVIQ
210 220 230 240 250
ARIANYDPAE IRFNLMVICK DKKASLLTRE DLTDEEKAAS IAVEDEKRLR
260 270 280 290 300
WKRENQLRRH NFVGLFVELS KLLVKDRIDK NTWNSTLETA KAKYASQKRP
Length:300
Mass (Da):34,196
Last modified:May 1, 2000 - v1
Checksum:i93B13076EF2EDB80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB52608.1.
PIRiT40434.
RefSeqiNP_595456.1. NM_001021366.2.

Genome annotation databases

EnsemblFungiiSPBC409.06.1; SPBC409.06.1:pep; SPBC409.06.
GeneIDi2540956.
KEGGispo:SPBC409.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB52608.1.
PIRiT40434.
RefSeqiNP_595456.1. NM_001021366.2.

3D structure databases

ProteinModelPortaliQ9UUB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277472. 63 interactions.
IntActiQ9UUB6. 1 interaction.
MINTiMINT-4711794.
STRINGi4896.SPBC409.06.1.

Protein family/group databases

MEROPSiC12.009.

Proteomic databases

MaxQBiQ9UUB6.
PaxDbiQ9UUB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC409.06.1; SPBC409.06.1:pep; SPBC409.06.
GeneIDi2540956.
KEGGispo:SPBC409.06.

Organism-specific databases

EuPathDBiFungiDB:SPBC409.06.
PomBaseiSPBC409.06. uch2.

Phylogenomic databases

eggNOGiNOG321645.
HOGENOMiHOG000203918.
InParanoidiQ9UUB6.
KOiK05610.
OMAiLIREVHN.
OrthoDBiEOG7FBRWB.
PhylomeDBiQ9UUB6.

Miscellaneous databases

NextBioi20802071.
PROiQ9UUB6.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast."
    Stone M., Hartmann-Petersen R., Seeger M., Bech-Otschir D., Wallace M., Gordon C.
    J. Mol. Biol. 344:697-706(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RPN10, SUBCELLULAR LOCATION.
  3. "Identification of a 26S proteasome-associated UCH in fission yeast."
    Li T., Naqvi N.I., Yang H., Teo T.S.
    Biochem. Biophys. Res. Commun. 272:270-275(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiUBLH2_SCHPO
AccessioniPrimary (citable) accession number: Q9UUB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 1, 2000
Last modified: June 24, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.