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Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

uch2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei83NucleophileBy similarity1
Active sitei159Proton donorBy similarity1
Sitei174Important for enzyme activityBy similarity1

GO - Molecular functioni

  • NEDD8-specific protease activity Source: PomBase
  • thiol-dependent ubiquitin-specific protease activity Source: PomBase

GO - Biological processi

  • cytoplasm protein quality control by the ubiquitin-proteasome system Source: PomBase
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: PomBase
  • protein deneddylation Source: PomBase
  • protein deubiquitination Source: PomBase

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-SPO-5689603 UCH proteinases

Protein family/group databases

MEROPSiC12.009

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Gene namesi
Name:uch2
ORF Names:SPBC409.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC409.06
PomBaseiSPBC409.06 uch2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002345611 – 300Ubiquitin carboxyl-terminal hydrolase 2Add BLAST300

Proteomic databases

MaxQBiQ9UUB6
PaxDbiQ9UUB6
PRIDEiQ9UUB6

PTM databases

iPTMnetiQ9UUB6

Interactioni

Subunit structurei

Component of the 26S proteasome. Interacts with rpn10.1 Publication

Protein-protein interaction databases

BioGridi277472, 64 interactors
IntActiQ9UUB6, 1 interactor
STRINGi4896.SPBC409.06.1

Structurei

3D structure databases

ProteinModelPortaliQ9UUB6
SMRiQ9UUB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

HOGENOMiHOG000203918
InParanoidiQ9UUB6
KOiK05610
OMAiTIQNACA
OrthoDBiEOG092C36PI
PhylomeDBiQ9UUB6

Family and domain databases

Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR001578 Peptidase_C12_UCH
IPR036959 Peptidase_C12_UCH_sf
IPR017390 Ubiquitinyl_hydrolase_UCH37
PANTHERiPTHR10589 PTHR10589, 1 hit
PfamiView protein in Pfam
PF01088 Peptidase_C12, 1 hit
PIRSFiPIRSF038120 Ubiquitinyl_hydrolase_UCH37, 1 hit
PRINTSiPR00707 UBCTHYDRLASE

Sequencei

Sequence statusi: Complete.

Q9UUB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWTTIESDA GVFTDLIENL GVKDVEVDEL YSLDVDSLRQ FPDIYGIIFL
60 70 80 90 100
FKWNSKVDKP DGTMDYDSMD NIFFAKQVIN NACATQALLS VLLNHSDEID
110 120 130 140 150
LGTTLSEFKD FSKTLPPELK GEALGNSEHI RCCHNSFARS DPFISEEVRA
160 170 180 190 200
ATDEDEVYHF IAYTNINNVF YELDGLQAAP INHGSCTKEE FAEKAVSVIQ
210 220 230 240 250
ARIANYDPAE IRFNLMVICK DKKASLLTRE DLTDEEKAAS IAVEDEKRLR
260 270 280 290 300
WKRENQLRRH NFVGLFVELS KLLVKDRIDK NTWNSTLETA KAKYASQKRP
Length:300
Mass (Da):34,196
Last modified:May 1, 2000 - v1
Checksum:i93B13076EF2EDB80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAB52608.1
PIRiT40434
RefSeqiNP_595456.1, NM_001021366.2

Genome annotation databases

EnsemblFungiiSPBC409.06.1; SPBC409.06.1:pep; SPBC409.06
GeneIDi2540956
KEGGispo:SPBC409.06

Similar proteinsi

Entry informationi

Entry nameiUBLH2_SCHPO
AccessioniPrimary (citable) accession number: Q9UUB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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