Reviewed, UniProtKB/Swiss-Prot Q9UUB4 (PUR7_SCHPO)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole-succinocarboxamide synthase
    EC=6.3.2.6
Alternative name(s):
    SAICAR synthetase

Gene names

Name:	ade7
ORF Names:	SPBC409.10

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	adenine biosynthetic process Inferred from mutant phenotype. Source: GeneDB_SPombe purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Phosphoribosylaminoimidazole-succinocarboxamide synthase		PRO_0000100928

Sequences

	Sequence		Length	Mass (Da)
	Q9UUB4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: BB1BF35D1F48E8A3	FASTA	299	33,914
	10 20 30 40 50 60 MALLKTSLDA PFTKIATGKV RDLYECVEFP DDLLFVATDR ISAYDFIMEN GIPEKGKVLT 70 80 90 100 110 120 KISEFWFDVL KPHVQTHLIT SRWEELPPVI TKHEELKDRS MLVKKYKILP IEAIVRGYIT 130 140 150 160 170 180 GSAWKEYQKQ GTVHGLNVPT GMKEAEAFPE PLFTPSTKAA EGHDENIHPD EVSKIVGPEL 190 200 210 220 230 240 AKQVAETSVK LYKIARDVAL KKGIIIADTK FEFGVDETTN KIVLVDEVLT PDSSRFWLAS 250 260 270 280 290 DYTVGKSPDS FDKQYLRNWL TANNLANKPN VSLPEDVVEA TRRKYVEAYE MITGKKWSY

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAB52612.1.
PIR	T40437.
RefSeq	NP_595460.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UUB4.
Genome annotation databases
GeneID	2541029.
GenomeReviews	Gene locus ade7 in contig CU329671_GR.
KEGG	spo:SPBC409.10.
NMPDR	fig|4896.1.peg.1326.
Organism-specific databases
GeneDB_Spombe	SPBC409.10.
Phylogenomic databases
OMA	SSRIWDG
OrthoDB	EOG9CNS78
Enzyme and pathway databases
BRENDA	6.3.2.6. 653.
Gene expression databases
ArrayExpress	Q9UUB4.
Family and domain databases
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synt. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00081. purC. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_SCHPO

Accession

Primary (citable) accession number: Q9UUB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)