Q9UTS7 (ASPG2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-asparaginase 2
EC=3.5.1.1

Alternative name(s):
L-asparagine amidohydrolase 2

Gene names

ORF Names:

SPAC977.12

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subcellular location	Secreted › cell wall By similarity.
Sequence similarities	Belongs to the asparaginase 1 family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparagine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from direct assay. Source: GeneDB_Spombe cell surface Non-traceable author statement. Source: GeneDB_Spombe cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 356	334	Probable L-asparaginase 2		PRO_0000002364
Regions
	Region	126 – 127	2	Substrate binding By similarity
Sites
	Active site	46	1	O-isoaspartyl threonine intermediate By similarity
	Binding site	93	1	Substrate By similarity
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9UTS7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E7935A592DB2BFDA	FASTA	356	38,115
	10 20 30 40 50 60 MWGFIVTCGI FLVLLCQLRL LSKRKSKSTP YNALLPNVTV FAMGGTIAGC ANSSLEIVNY 70 80 90 100 110 120 IPGSVGIEKL IEAVPAIKAI ANINGVQVTN MGSENLTPAD VLKLAKLILA EVAKPNVHGI 130 140 150 160 170 180 VITHGTDSLE ETAMFLDLTI STAKPIVVVG AMRPSTAIGA DGPMNLLNAV AVASSNQSMG 190 200 210 220 230 240 RGTLVLLNDR IGSAFYTTKT NGNTLDTFKS YEAGSLGIVL NQKPFYFFSP AVPTGKVFFD 250 260 270 280 290 300 IYNIKQLPRV DILYGYQGLN PKLAESAVHL GAKGLVLAAM GATSWTDDGN EVISSLIREH 310 320 330 340 350 NIPVVYSHRT AEGYSSNSCL GIPSYFLNPQ KARYMLMLAI SSGYSIRDIE DLFSIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mechanisms of sod2 gene amplification in Schizosaccharomyces pombe." Albrecht E.B., Hunyady A.B., Stark G.R., Patterson T.E. Mol. Biol. Cell 11:873-886(2000) [PubMed: 10712506] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF192974 Genomic DNA. Translation: AAF13924.1. CU329670 Genomic DNA. Translation: CAB69634.1.
PIR	T50284.
RefSeq	NP_592784.1. NM_001018184.1.
3D structure databases
ProteinModelPortal	Q9UTS7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9UTS7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC977.12.1; SPAC977.12.1:pep; SPAC977.12.
GeneID	2543314.
KEGG	spo:SPAC977.12.
NMPDR	fig|4896.1.peg.2754.
Organism-specific databases
GeneDB_Spombe	SPAC977.12.
Phylogenomic databases
eggNOG	fuNOG07200.
GeneTree	EFGT00050000004559.
HOGENOM	HBG497495.
OMA	GGYLNPQ.
OrthoDB	EOG4P5PJT.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-000529-MONOMER.
Gene expression databases
ArrayExpress	Q9UTS7.
Family and domain databases
InterPro	IPR004550. AsnASE_II. IPR006034. Asparaginase/glutaminase. [Graphical view]
KO	K01424.
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PIRSF	PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTS	PR00139. ASNGLNASE.
SMART	SM00870. Asparaginase. 1 hit. [Graphical view]
SUPFAM	SSF53774. Asp/Glutamnse. 1 hit.
TIGRFAMs	TIGR00520. AsnASE_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPG2_SCHPO

Accession

Primary (citable) accession number: Q9UTS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program