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Protein

Alkyltransferase-like protein 1

Gene

atl1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA damage recognition. Binds DNA containing O(6)-methylguanine and larger O(6)-alkylguanine adducts. The DNA is bent, the damaged base is rotated out of the DNA duplex into a hydrophobic binding pocket (nucleotide flipping), with Arg-39 donating a hydrogen bond to the orphaned cytosine to stabilize the extrahelical DNA conformation. This structural change in DNA presents the lesion to the nucleotide excision repair (NER) pathway (PubMed:16679453, PubMed:19516334, PubMed:23112169). The affinity for O(6)-alkylguanine adducts increases with the size of the alkyl group. Low affinity small O(6)-alkylguanines are directed to the global genome repair pathway of NER via rhp7-rhp16 and rhp41-rhp23, while strong binding to bulky O(6)-alkylguanines stalls the transcription machinery and diverts the damage to the transcription-coupled repair pathway of NER via rhp26 (PubMed:22658721).4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei25 – 251Required for phosphate rotation/nucleotide flipping1 Publication
Sitei39 – 391Arg finger, required for nucleotide flipping1 Publication
Sitei69 – 691Critical for recognition of O(6)-alkylguanines, probes the electrostatic potential of the flipped base to distinguish between O(6)-alkylguanine and guanine1 Publication

GO - Molecular functioni

  • catalytic activity Source: InterPro
  • damaged DNA binding Source: PomBase
  • O6-alkylguanine-DNA binding Source: PomBase

GO - Biological processi

  • DNA dealkylation involved in DNA repair Source: PomBase
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alkyltransferase-like protein 11 Publication
Gene namesi
Name:atl11 Publication
ORF Names:SPAC1250.04cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1250.04c.
PomBaseiSPAC1250.04c. atl1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691R → A or F: Reduces discrimination of modified bases 10-100-fold and increases sensitivity toward alkylating agents. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 108108Alkyltransferase-like protein 1PRO_0000249237Add
BLAST

Proteomic databases

MaxQBiQ9UTN9.

Interactioni

Protein-protein interaction databases

BioGridi278199. 18 interactions.
DIPiDIP-60450N.
MINTiMINT-4710996.

Structurei

Secondary structure

1
108
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Beta strandi22 – 243Combined sources
Helixi25 – 317Combined sources
Helixi35 – 373Combined sources
Helixi38 – 469Combined sources
Helixi56 – 583Combined sources
Turni62 – 643Combined sources
Beta strandi70 – 723Combined sources
Helixi73 – 8412Combined sources
Beta strandi90 – 923Combined sources
Beta strandi93 – 953Combined sources
Helixi101 – 1044Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GVAX-ray2.00A/B1-108[»]
3GX4X-ray2.70X1-108[»]
3GYHX-ray2.80X1-108[»]
4ENJX-ray3.10A1-108[»]
4ENKX-ray3.04A1-108[»]
4ENMX-ray2.84A1-108[»]
4ENNX-ray2.84A/B1-108[»]
4HDUX-ray2.85A1-108[»]
4HDVX-ray2.70A1-108[»]
ProteinModelPortaliQ9UTN9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UTN9.

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family. ATL subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000244138.
InParanoidiQ9UTN9.
KOiK07443.
OMAiFKNINEN.
OrthoDBiEOG092C5L5L.
PhylomeDBiQ9UTN9.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UTN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMDEFYTKV YDAVCEIPYG KVSTYGEIAR YVGMPSYARQ VGQAMKHLHP
60 70 80 90 100
ETHVPWHRVI NSRGTISKRD ISAGEQRQKD RLEEEGVEIY QTSLGEYKLN

LPEYMWKP
Length:108
Mass (Da):12,670
Last modified:May 1, 2000 - v1
Checksum:i7F8B3BA12CED07F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB54827.1.
PIRiT37558.
RefSeqiNP_594858.1. NM_001020287.2.

Genome annotation databases

EnsemblFungiiSPAC1250.04c.1; SPAC1250.04c.1:pep; SPAC1250.04c.
GeneIDi2541704.
KEGGispo:SPAC1250.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB54827.1.
PIRiT37558.
RefSeqiNP_594858.1. NM_001020287.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GVAX-ray2.00A/B1-108[»]
3GX4X-ray2.70X1-108[»]
3GYHX-ray2.80X1-108[»]
4ENJX-ray3.10A1-108[»]
4ENKX-ray3.04A1-108[»]
4ENMX-ray2.84A1-108[»]
4ENNX-ray2.84A/B1-108[»]
4HDUX-ray2.85A1-108[»]
4HDVX-ray2.70A1-108[»]
ProteinModelPortaliQ9UTN9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278199. 18 interactions.
DIPiDIP-60450N.
MINTiMINT-4710996.

Proteomic databases

MaxQBiQ9UTN9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1250.04c.1; SPAC1250.04c.1:pep; SPAC1250.04c.
GeneIDi2541704.
KEGGispo:SPAC1250.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1250.04c.
PomBaseiSPAC1250.04c. atl1.

Phylogenomic databases

HOGENOMiHOG000244138.
InParanoidiQ9UTN9.
KOiK07443.
OMAiFKNINEN.
OrthoDBiEOG092C5L5L.
PhylomeDBiQ9UTN9.

Miscellaneous databases

EvolutionaryTraceiQ9UTN9.
PROiQ9UTN9.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01035. DNA_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF46767. SSF46767. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiATL1_SCHPO
AccessioniPrimary (citable) accession number: Q9UTN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Does not have alkyltransferase activity. A tryptophan residue replaces the cysteine at the known active site of MGMT.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.