Reviewed, UniProtKB/Swiss-Prot Q9UTK9 (YIVH_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase C144.17c
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

ORF Names:

SPAC144.17c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate By similarity.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. Ref.2
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred by curator. Source: GeneDB_SPombe
Cellular component	cytosol Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Putative 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase C144.17c		PRO_0000318493
Regions
	Nucleotide binding	59 – 66	8	ATP By similarity
	Region	261 – 410	150	Fructose-2,6-bisphosphatase By similarity
Sites
	Active site	142	1	Potential
	Binding site	207	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	25	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9UTK9-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7D4E8FAE2EE223AE	FASTA	432	50,214
	10 20 30 40 50 60 MSRRNSIAVE QAINPNMLRA ANRSSEALLD NEKFGGVQPY STESGMLFHA GKLVIIMSGL 70 80 90 100 110 120 PARGKSNIAV SIDRYLRWLG FNCRFYSLAK YIDERTREMT SSPVKSAASE NHVFSRNDTI 130 140 150 160 170 180 ERCLADLEIF LLKEKGQVAI YDATNGTRRT RRILYDRFKN CGFKILFIES LCNKEDVINA 190 200 210 220 230 240 NIQEAIHVSE EFRNWDLEMA EKEYCRRIDI LKCHYETIDE KDYSFVKMIN FAETIIANKS 250 260 270 280 290 300 NEGYLLSRIL FLLMNMTLAR KRIFLVPKAS MRPLKLREPE DDIENRQFSE YVADRIRKSF 310 320 330 340 350 360 PDVSFKNLHV LSCMEDSVMS PFRELGSVTS SMSSLSPILM DSFGDDLQKL KETYGEEEYN 370 380 390 400 410 420 LYLVDPYRYR VKRKESFYDL AVRLEPLILE LGREQRDVLL IGSKSIIRVF YGYYMNVPAK 430 VRFLTLSIAT YK

References

[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB59697.1.
PIR	T37684.
RefSeq	NP_594678.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2542894.
KEGG	spo:SPAC144.17c.
NMPDR	fig|4896.1.peg.4648.
Organism-specific databases
GeneDB_Spombe	SPAC144.17c.
Phylogenomic databases
OMA	Q9UTK9. SYHDLAV.
Enzyme and pathway databases
BRENDA	2.7.1.105. 653. 3.1.3.46. 653.
Gene expression databases
ArrayExpress	Q9UTK9.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. [Graphical view]
PRINTS	PR00991. 6PFRUCTKNASE.
ProtoNet	Search...

Entry information

Entry name

YIVH_SCHPO

Accession

Primary (citable) accession number: Q9UTK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)