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Protein

Nucleoporin nup189

Gene

nup189

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Protein family/group databases

MEROPSiS59.A07.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin nup189
Alternative name(s):
Nuclear pore protein nup189
Gene namesi
Name:nup189
ORF Names:SPAC1486.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1486.05.
PomBaseiSPAC1486.05. nup189.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: PomBase
  • nuclear periphery Source: PomBase
  • nuclear pore Source: PomBase
  • nuclear pore outer ring Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17781778Nucleoporin nup189PRO_0000204857Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei695 – 6951Phosphoserine1 Publication
Modified residuei1022 – 10221Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9UTK4.

PTM databases

iPTMnetiQ9UTK4.

Interactioni

Subunit structurei

Monomer. Interacts with ned1 and raf1.2 Publications

Protein-protein interaction databases

BioGridi278047. 3 interactions.
IntActiQ9UTK4. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9UTK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini793 – 934142Peptidase S59PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 625617Gly-richAdd
BLAST
Compositional biasi233 – 596364Thr-richAdd
BLAST
Compositional biasi393 – 44250Asn-richAdd
BLAST

Domaini

Contains G-L-F-G repeats.

Sequence similaritiesi

Belongs to the nucleoporin GLFG family.Curated
Contains 1 peptidase S59 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiQ9UTK4.
KOiK14297.
OMAiAWIWVAK.
OrthoDBiEOG7QRR34.
PhylomeDBiQ9UTK4.

Family and domain databases

Gene3Di3.30.1610.10. 1 hit.
InterProiIPR025574. Nucleoporin_FG_rpt.
IPR021967. Nup96.
IPR007230. Peptidase_S59.
[Graphical view]
PfamiPF04096. Nucleoporin2. 1 hit.
PF13634. Nucleoporin_FG. 9 hits.
PF12110. Nup96. 1 hit.
[Graphical view]
SUPFAMiSSF82215. SSF82215. 1 hit.
PROSITEiPS51434. NUP_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UTK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGQNNSSGF GGGTGAFGQN NQQTGGLFGS NSNTPGNTLF GSQNTSTTGF
60 70 80 90 100
GQNTTQPLFG SNTNGGLFGN RNNTTTTGGT GFGMSSGTGM FGQSNTPAFG
110 120 130 140 150
GTNNATNPSG GGLFGSNTAN NNANTGTSFS FGSNAGSTGF GSQGTGGGLF
160 170 180 190 200
GSSTTPATTN AFGTSGFVSS NANAVNGTAN PPYAVTSEKD PQTNGTSVFQ
210 220 230 240 250
SITCMPAYRS YSFEELRLQD YNQGRRFGNA SSTNTTSAFG STPAFGASTT
260 270 280 290 300
PFGQNLSGTT NNATPFGTSN ATNTTPGSGL FGGGSAFGSN TTNTGFGSGT
310 320 330 340 350
NNASGGLFGQ NNNTTSTPST GLFGGSTFNQ QKPAFSGFGS TTNTTNTGTG
360 370 380 390 400
TGLFGSNNAT NTGTGQTTGG LFGGAATGTG TGFGSSTGGF GSNTNNQPNS
410 420 430 440 450
GTMGTGLFGF GANNNTANNN TAPTSTFGGN NSSNFSFGAN NNAATKPSGF
460 470 480 490 500
GFGSTTTTPA SGGFSFGQNA NNAPKPAFGS TATTAPKPAG TGLFGGLGAG
510 520 530 540 550
ANTNTATNAT GTGGSLFGNA NTAGSNMFGS ANSSTPGTGL FGSTQTNNAT
560 570 580 590 600
SNTGTGLFGS NNANTTNTGG SLFNKPSTTT GGLFGNTTAQ QPSTTTSGLF
610 620 630 640 650
GASNTNNQAQ TSNFGTGLFG GSQAGQQQQP LQASIDQNPY GNNPLFSSTT
660 670 680 690 700
SQVAPTSIQE PIASPLTSKP TPKKAASLPQ FWLSPRSHNT ARLASISSFA
710 720 730 740 750
KSAVMNSTSA SGKPKSLHLF DSLNDDVLLS ADAFTPRQNI KKLVITHKIS
760 770 780 790 800
KDDILQNGVK NGNDAKSDSK VQEKAPQNEA DGSLKKDEHV VLSDDYWMKP
810 820 830 840 850
SIEELSKYPK EKLCSVHQFS VGRTGYGQVA FLKPVDLSGF EKLEDIPGKV
860 870 880 890 900
VVFERKICAV YPVEGSSPPL GEGLNVPAII TLEKTWPLSR ETREPIKDPQ
910 920 930 940 950
NPRYIQHVKR LHRIKDTEFI DFNDGKWIFK VQHFSRYGLL DDEEEENDMS
960 970 980 990 1000
STSNEAGNLK KYDQPNLKVS GKNDSFVTHH TPGAFPNDSK NKELNRHFLK
1010 1020 1030 1040 1050
VDDSAPLDDT FMSKKVKLDF SSDSNVSERG DYDDNAKKVD EVISIEKVDG
1060 1070 1080 1090 1100
YSKENNVPLS EDDLSNSSES SNESVYSLVE ESDASLAADN MDIEDISEES
1110 1120 1130 1140 1150
DREELSSMRF GAQDFHGLVV TDNWRDQLNL SVQRSALIKA AFPESQSNAN
1160 1170 1180 1190 1200
LKNSRGIYYN EHDLVTDIFG NQNLDTDRPW QSLDKPGAFI PSKFHFTANG
1210 1220 1230 1240 1250
SCIYVLKSSD VKIRSIYDFI PTKDPNGTKL LEYQLDQTEV YLDLSGTHAA
1260 1270 1280 1290 1300
SPRSSMTVKP LSLCSSGYES IVWDLTSILF DPKNYSLPSE LSSEAREVLY
1310 1320 1330 1340 1350
QKLVRESLSE WITKTLEHET TTLAKEAETS EERIYILLTG NLIGQACEEA
1360 1370 1380 1390 1400
VQSQNNRLST LIPLVNSDVD IQQEVKQQLE EWRKHGDLPF INKFTRLIFE
1410 1420 1430 1440 1450
LLSGNTDIAE GCGTKGDEDY VQSIPITKNM TWLRAFGLKL WYNTDISIGE
1460 1470 1480 1490 1500
AMQLYVESLQ KFPEIMQKPI ATSAVQGIEV YDIIYLLLKA YAMGTSLEEL
1510 1520 1530 1540 1550
TIPESAKCSP LNYRVVWQLA IYLSKARSLC DFSDRVVDIN MAEDLKPISV
1560 1570 1580 1590 1600
HSDQLTLAYA SQLEASGQWL WSLFVLLHLE NVETRTSTIT SCLARNLRGG
1610 1620 1630 1640 1650
LGAGAVEMIE KLCIPESWLN EAKALYARYV GDHLNELYFL QEAALYEDAH
1660 1670 1680 1690 1700
KVLLDTLAPQ AVISGNKTQL KKALEGFNGQ TDGLASWRFG GQIYSDYLDL
1710 1720 1730 1740 1750
LEGNFDANQE LKLFTLRKIS VALKELNATN LLQKAALHKI SRFVNALCNE
1760 1770
ESLTDAICNL PLPLADSLAN LQNISVQF
Length:1,778
Mass (Da):189,576
Last modified:May 1, 2000 - v1
Checksum:i1D2AED57D927ADC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB62415.1.
D89145 mRNA. Translation: BAA13807.1.
PIRiT42421.
T50074.
RefSeqiNP_594093.1. NM_001019517.2.

Genome annotation databases

GeneIDi2541547.
KEGGispo:SPAC1486.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB62415.1.
D89145 mRNA. Translation: BAA13807.1.
PIRiT42421.
T50074.
RefSeqiNP_594093.1. NM_001019517.2.

3D structure databases

ProteinModelPortaliQ9UTK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278047. 3 interactions.
IntActiQ9UTK4. 3 interactions.

Protein family/group databases

MEROPSiS59.A07.

PTM databases

iPTMnetiQ9UTK4.

Proteomic databases

MaxQBiQ9UTK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2541547.
KEGGispo:SPAC1486.05.

Organism-specific databases

EuPathDBiFungiDB:SPAC1486.05.
PomBaseiSPAC1486.05. nup189.

Phylogenomic databases

InParanoidiQ9UTK4.
KOiK14297.
OMAiAWIWVAK.
OrthoDBiEOG7QRR34.
PhylomeDBiQ9UTK4.

Miscellaneous databases

NextBioi20802644.
PROiQ9UTK4.

Family and domain databases

Gene3Di3.30.1610.10. 1 hit.
InterProiIPR025574. Nucleoporin_FG_rpt.
IPR021967. Nup96.
IPR007230. Peptidase_S59.
[Graphical view]
PfamiPF04096. Nucleoporin2. 1 hit.
PF13634. Nucleoporin_FG. 9 hits.
PF12110. Nup96. 1 hit.
[Graphical view]
SUPFAMiSSF82215. SSF82215. 1 hit.
PROSITEiPS51434. NUP_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Identification of open reading frames in Schizosaccharomyces pombe cDNAs."
    Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.
    DNA Res. 4:363-369(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1428-1778.
    Strain: PR745.
  3. "The fission yeast Nup107-120 complex functionally interacts with the small GTPase Ran/Spi1 and is required for mRNA export, nuclear pore distribution, and proper cell division."
    Bai S.W., Rouquette J., Umeda M., Faigle W., Loew D., Sazer S., Doye V.
    Mol. Cell. Biol. 24:6379-6392(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "An evolutionarily conserved fission yeast protein, Ned1, implicated in normal nuclear morphology and chromosome stability, interacts with Dis3, Pim1/RCC1 and an essential nucleoporin."
    Tange Y., Hirata A., Niwa O.
    J. Cell Sci. 115:4375-4385(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, INTERACTION WITH NED1.
  5. "Identification of genes encoding putative nucleoporins and transport factors in the fission yeast Schizosaccharomyces pombe: a deletion analysis."
    Chen X.Q., Du X., Liu J., Balasubramanian M.K., Balasundaram D.
    Yeast 21:495-509(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "The Clr7 and Clr8 directionality factors and the Pcu4 cullin mediate heterochromatin formation in the fission yeast Schizosaccharomyces pombe."
    Thon G., Hansen K.R., Altes S.P., Sidhu D., Singh G., Verhein-Hansen J., Bonaduce M.J., Klar A.J.
    Genetics 171:1583-1595(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAF1.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-695 AND SER-1022, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiNU189_SCHPO
AccessioniPrimary (citable) accession number: Q9UTK4
Secondary accession number(s): P78796
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.