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Protein

Nucleoporin nup189

Gene

nup189

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (PubMed:15116432). Nup189 is autocatalytically cleaved in vivo in 2 polypeptides which assume different functions in the NPC (PubMed:26137436). Nup98 as one of the FG repeat nucleoporins participates in karyopherin interactions and contains part of the autocatalytic cleavage activity. Nup96 as part of the NUP84 complex is involved in nuclear poly(A)+ RNA and tRNA export (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processmRNA transport, Protein transport, Translocation, Transport

Protein family/group databases

MEROPSiS59.A07.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin nup189 (EC:3.4.21.-)
Alternative name(s):
Nuclear pore protein nup189
Cleaved into the following 2 chains:
Gene namesi
Name:nup189
Synonyms:nup96, nup98
ORF Names:SPAC1486.05Imported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1486.05.
PomBaseiSPAC1486.05. nup189.

Subcellular locationi

Nucleoporin nup98 :
Nucleoporin nup96 :

GO - Cellular componenti

  • nuclear membrane Source: PomBase
  • nuclear periphery Source: PomBase
  • nuclear pore Source: PomBase
  • nuclear pore cytoplasmic filaments Source: GO_Central
  • nuclear pore outer ring Source: PomBase

Keywords - Cellular componenti

Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi964S → A: Prevents autocatalytic cleavage and produces a fully functional fusion protein. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048571 – 963Nucleoporin nup98Add BLAST963
ChainiPRO_0000438989964 – 1807Nucleoporin nup96Add BLAST844

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei724Phosphoserine1 Publication1
Modified residuei1051Phosphoserine1 Publication1

Post-translational modificationi

Nup189 is autocatalytically cleaved in nup98 and nup96.1 Publication

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein

Proteomic databases

PRIDEiQ9UTK4.

PTM databases

iPTMnetiQ9UTK4.

Expressioni

Gene expression databases

ExpressionAtlasiQ9UTK4. differential.

Interactioni

Subunit structurei

Nucleoporin nup98: Interacts (via G-L-F-G repeats) with rpn15/dss1 (PubMed:15990877). Nucleoporin nup98: Interacts with raf1 (PubMed:16157682). Nucleoporin nup96: Interacts with ned1 (PubMed:12376568).3 Publications

Protein-protein interaction databases

BioGridi278047. 3 interactors.
IntActiQ9UTK4. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ9UTK4.
SMRiQ9UTK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati26 – 29GLFG 1Curated4
Repeati66 – 69GLFG 2Curated4
Repeati112 – 115GLFG 3Curated4
Repeati152 – 155GLFG 4Curated4
Repeati177 – 180GLFG 5Curated4
Repeati308 – 311GLFG 6Curated4
Repeati335 – 338GLFG 7Curated4
Repeati350 – 353GLFG 8Curated4
Repeati381 – 384GLFG 9Curated4
Repeati399 – 402GLFG 10Curated4
Repeati435 – 438GLFG 11Curated4
Repeati521 – 524GLFG 12Curated4
Repeati585 – 588GLFG 13Curated4
Repeati611 – 614GLFG 14Curated4
Repeati627 – 630GLFG 15Curated4
Repeati646 – 649GLFG 16Curated4
Domaini822 – 963Peptidase S59PROSITE-ProRule annotationAdd BLAST142

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 654Gly-richPROSITE-ProRule annotationAdd BLAST646
Compositional biasi262 – 625Thr-richPROSITE-ProRule annotationAdd BLAST364
Compositional biasi422 – 471Asn-richPROSITE-ProRule annotationAdd BLAST50

Domaini

Contains G-L-F-G repeats.Curated

Sequence similaritiesi

Belongs to the nucleoporin GLFG family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

InParanoidiQ9UTK4.
KOiK14297.
OMAiAWIWVAK.
OrthoDBiEOG092C0BHV.
PhylomeDBiQ9UTK4.

Family and domain databases

Gene3Di3.30.1610.10. 1 hit.
InterProiView protein in InterPro
IPR025574. Nucleoporin_FG_rpt.
IPR021967. Nup96.
IPR007230. Peptidase_S59.
PfamiView protein in Pfam
PF04096. Nucleoporin2. 1 hit.
PF13634. Nucleoporin_FG. 4 hits.
PF12110. Nup96. 1 hit.
SUPFAMiSSF82215. SSF82215. 1 hit.
PROSITEiView protein in PROSITE
PS51434. NUP_C. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UTK4-1) [UniParc]FASTAAdd to basket
Also known as: long1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGQNNSSGF GGGTGAFGQN NQQTGGLFGS NSNTPGNTLF GSQNTSTTGF
60 70 80 90 100
GQNTTQPLFG SNTNGGLFGN RNNTTTTGGT GFGMSSGTGM FGQSNTPAFG
110 120 130 140 150
GTNNATNPSG GGLFGSNTAN NNANTGTSFS FGSNAGSTGF GNTASNTGTG
160 170 180 190 200
GGLFGSQNNA GNTAGNTGFG SQGTGGGLFG SSTTPATTNA FGTSGFVSSN
210 220 230 240 250
ANAVNGTANP PYAVTSEKDP QTNGTSVFQS ITCMPAYRSY SFEELRLQDY
260 270 280 290 300
NQGRRFGNAS STNTTSAFGS TPAFGASTTP FGQNLSGTTN NATPFGTSNA
310 320 330 340 350
TNTTPGSGLF GGGSAFGSNT TNTGFGSGTN NASGGLFGQN NNTTSTPSTG
360 370 380 390 400
LFGGSTFNQQ KPAFSGFGST TNTTNTGTGT GLFGSNNATN TGTGQTTGGL
410 420 430 440 450
FGGAATGTGT GFGSSTGGFG SNTNNQPNSG TMGTGLFGFG ANNNTANNNT
460 470 480 490 500
APTSTFGGNN SSNFSFGANN NAATKPSGFG FGSTTTTPAS GGFSFGQNAN
510 520 530 540 550
NAPKPAFGST ATTAPKPAGT GLFGGLGAGA NTNTATNATG TGGSLFGNAN
560 570 580 590 600
TAGSNMFGSA NSSTPGTGLF GSTQTNNATS NTGTGLFGSN NANTTNTGGS
610 620 630 640 650
LFNKPSTTTG GLFGNTTAQQ PSTTTSGLFG ASNTNNQAQT SNFGTGLFGG
660 670 680 690 700
SQAGQQQQPL QASIDQNPYG NNPLFSSTTS QVAPTSIQEP IASPLTSKPT
710 720 730 740 750
PKKAASLPQF WLSPRSHNTA RLASISSFAK SAVMNSTSAS GKPKSLHLFD
760 770 780 790 800
SLNDDVLLSA DAFTPRQNIK KLVITHKISK DDILQNGVKN GNDAKSDSKV
810 820 830 840 850
QEKAPQNEAD GSLKKDEHVV LSDDYWMKPS IEELSKYPKE KLCSVHQFSV
860 870 880 890 900
GRTGYGQVAF LKPVDLSGFE KLEDIPGKVV VFERKICAVY PVEGSSPPLG
910 920 930 940 950
EGLNVPAIIT LEKTWPLSRE TREPIKDPQN PRYIQHVKRL HRIKDTEFID
960 970 980 990 1000
FNDGKWIFKV QHFSRYGLLD DEEEENDMSS TSNEAGNLKK YDQPNLKVSG
1010 1020 1030 1040 1050
KNDSFVTHHT PGAFPNDSKN KELNRHFLKV DDSAPLDDTF MSKKVKLDFS
1060 1070 1080 1090 1100
SDSNVSERGD YDDNAKKVDE VISIEKVDGY SKENNVPLSE DDLSNSSESS
1110 1120 1130 1140 1150
NESVYSLVEE SDASLAADNM DIEDISEESD REELSSMRFG AQDFHGLVVT
1160 1170 1180 1190 1200
DNWRDQLNLS VQRSALIKAA FPESQSNANL KNSRGIYYNE HDLVTDIFGN
1210 1220 1230 1240 1250
QNLDTDRPWQ SLDKPGAFIP SKFHFTANGS CIYVLKSSDV KIRSIYDFIP
1260 1270 1280 1290 1300
TKDPNGTKLL EYQLDQTEVY LDLSGTHAAS PRSSMTVKPL SLCSSGYESI
1310 1320 1330 1340 1350
VWDLTSILFD PKNYSLPSEL SSEAREVLYQ KLVRESLSEW ITKTLEHETT
1360 1370 1380 1390 1400
TLAKEAETSE ERIYILLTGN LIGQACEEAV QSQNNRLSTL IPLVNSDVDI
1410 1420 1430 1440 1450
QQEVKQQLEE WRKHGDLPFI NKFTRLIFEL LSGNTDIAEG CGTKGDEDYV
1460 1470 1480 1490 1500
QSIPITKNMT WLRAFGLKLW YNTDISIGEA MQLYVESLQK FPEIMQKPIA
1510 1520 1530 1540 1550
TSAVQGIEVY DIIYLLLKAY AMGTSLEELT IPESAKCSPL NYRVVWQLAI
1560 1570 1580 1590 1600
YLSKARSLCD FSDRVVDINM AEDLKPISVH SDQLTLAYAS QLEASGQWLW
1610 1620 1630 1640 1650
SLFVLLHLEN VETRTSTITS CLARNLRGGL GAGAVEMIEK LCIPESWLNE
1660 1670 1680 1690 1700
AKALYARYVG DHLNELYFLQ EAALYEDAHK VLLDTLAPQA VISGNKTQLK
1710 1720 1730 1740 1750
KALEGFNGQT DGLASWRFGG QIYSDYLDLL EGNFDANQEL KLFTLRKISV
1760 1770 1780 1790 1800
ALKELNATNL LQKAALHKIS RFVNALCNEE SLTDAICNLP LPLADSLANL

QNISVQF
Length:1,807
Mass (Da):192,202
Last modified:February 15, 2017 - v2
Checksum:i64F086C7A4C8D9A7
GO
Isoform 2 (identifier: Q9UTK4-2) [UniParc]FASTAAdd to basket
Also known as: short1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     991-996: YDQPNL → CISQRQ
     997-1807: Missing.

Show »
Length:996
Mass (Da):101,318
Checksum:iA8A606521D248CD7
GO

Sequence cautioni

The sequence CAB62415 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058774991 – 996YDQPNL → CISQRQ in isoform 2. 1 Publication6
Alternative sequenceiVSP_058775997 – 1807Missing in isoform 2. 1 PublicationAdd BLAST811

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LC037233 mRNA. Translation: BAR64199.1.
LC043101 mRNA. Translation: BAR64200.1.
CU329670 Genomic DNA. Translation: CAB62415.1. Sequence problems.
D89145 mRNA. Translation: BAA13807.1.
PIRiT42421.
T50074.

Genome annotation databases

KEGGispo:SPAC1486.05.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LC037233 mRNA. Translation: BAR64199.1.
LC043101 mRNA. Translation: BAR64200.1.
CU329670 Genomic DNA. Translation: CAB62415.1. Sequence problems.
D89145 mRNA. Translation: BAA13807.1.
PIRiT42421.
T50074.

3D structure databases

ProteinModelPortaliQ9UTK4.
SMRiQ9UTK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278047. 3 interactors.
IntActiQ9UTK4. 3 interactors.

Protein family/group databases

MEROPSiS59.A07.

PTM databases

iPTMnetiQ9UTK4.

Proteomic databases

PRIDEiQ9UTK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGispo:SPAC1486.05.

Organism-specific databases

EuPathDBiFungiDB:SPAC1486.05.
PomBaseiSPAC1486.05. nup189.

Phylogenomic databases

InParanoidiQ9UTK4.
KOiK14297.
OMAiAWIWVAK.
OrthoDBiEOG092C0BHV.
PhylomeDBiQ9UTK4.

Miscellaneous databases

PROiPR:Q9UTK4.

Gene expression databases

ExpressionAtlasiQ9UTK4. differential.

Family and domain databases

Gene3Di3.30.1610.10. 1 hit.
InterProiView protein in InterPro
IPR025574. Nucleoporin_FG_rpt.
IPR021967. Nup96.
IPR007230. Peptidase_S59.
PfamiView protein in Pfam
PF04096. Nucleoporin2. 1 hit.
PF13634. Nucleoporin_FG. 4 hits.
PF12110. Nup96. 1 hit.
SUPFAMiSSF82215. SSF82215. 1 hit.
PROSITEiView protein in PROSITE
PS51434. NUP_C. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNU189_SCHPO
AccessioniPrimary (citable) accession number: Q9UTK4
Secondary accession number(s): A0A0E3VYD1, A0A0E4FZX7, P78796
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: February 15, 2017
Last modified: May 10, 2017
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.