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Q9UTI7 (TYSY_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
ORF Names:SPAC15E1.04
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length625 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for both nuclear and mitochondrial DNA synthesis By similarity. UniProtKB P06785

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. UniProtKB P06785

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Subcellular location

Cytoplasm Ref.2.

Sequence similarities

In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.

In the C-terminal section; belongs to the thymidylate synthase family.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from sequence or structural similarity. Source: PomBase

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from direct assay Ref.2. Source: PomBase

   Molecular_functionflavin adenine dinucleotide binding

Inferred from sequence model. Source: PomBase

thymidylate synthase activity

Inferred from sequence or structural similarity. Source: PomBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 625625Probable thymidylate synthase
PRO_0000310825

Regions

Compositional bias229 – 25527Asp-rich

Sites

Active site4971 By similarity UniProtKB P13100

Sequences

Sequence LengthMass (Da)Tools
Q9UTI7 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: B3BC6034BF26F61E

FASTA62569,893
        10         20         30         40         50         60 
MSQPLHARFA TRAVKNPMIL EKERQLTDSK YHILVAATGS VAAIKLTLIV KSLLTYKGVD 

        70         80         90        100        110        120 
VQVVLTDPAR NFVEKEDLTA LGVNVYNNAD DWKNWDGLEC PITHIELRRW AHLLLIAPLS 

       130        140        150        160        170        180 
ANTMAKMANG LCDNLLTSLI RAWAPLKPIL LAPAMNTLMW TNPITQEHLS AISRIYKNSE 

       190        200        210        220        230        240 
FIMPIEKVLA CGDIGMGGMA EWRNIVGRVA DKLQLEQKSV LPNAVKNIDG QDDDSSEQTA 

       250        260        270        280        290        300 
AFEEYDDDDD DDVDDNEQSN SMIETSANAD ITPKASLLPS TTESSISKDH ETSQAPLGSE 

       310        320        330        340        350        360 
SVDTQASENV TTKPEPPVPF TSSEYRNTEE EQYLNLIRYI LENGQSRPDR TGTGTRSVFA 

       370        380        390        400        410        420 
PPQLRFSLRN NTLPLLTTKR VFLRGVLEEL LWFIHGDTNA NHLSEKGIHI WDGNGSREFL 

       430        440        450        460        470        480 
DSRGLTDRKV GDLGPIYGFQ WRHFGAQYVD CDTDYTNKGV DQLAQVISTL KLNPYDRRII 

       490        500        510        520        530        540 
LSAWNPLAIP EMALPPCHIF CQFYVSEPCK PGGKPQLSSM MYQRSADMGL GVPFNIASYS 

       550        560        570        580        590        600 
LLTHMIAHMC GYEAAEFVHV MGDCHIYNDH LEALQTQLER VPKAFPKLFF KRDAKDIGSI 

       610        620 
DSFSVDDFAV EGYNPYGPIK MKMSV 

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329670 Genomic DNA. Translation: CAB52423.1.
PIRT37719.
RefSeqNP_594305.1. NM_001019728.2.

3D structure databases

ProteinModelPortalQ9UTI7.
SMRQ9UTI7. Positions 327-625.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid279249. 3 interactions.
MINTMINT-4710571.
STRING4896.SPAC15E1.04-1.

Proteomic databases

MaxQBQ9UTI7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC15E1.04.1; SPAC15E1.04.1:pep; SPAC15E1.04.
GeneID2542801.
KEGGspo:SPAC15E1.04.

Organism-specific databases

PomBaseSPAC15E1.04.

Phylogenomic databases

eggNOGCOG0452.
KOK01598.
OMAITHIELR.
OrthoDBEOG7X6M9G.
PhylomeDBQ9UTI7.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
3.40.50.1950. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR003382. Flavoprotein.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF02441. Flavoprotein. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF52507. SSF52507. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20803844.
PROQ9UTI7.

Entry information

Entry nameTYSY_SCHPO
AccessionPrimary (citable) accession number: Q9UTI7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways