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Protein

Probable thymidylate synthase

Gene

SPAC15E1.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for both nuclear and mitochondrial DNA synthesis.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.By similarity

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei350dUMPBy similarity1
Active sitei497NucleophileBy similarity1
Binding sitei5275,10-methylenetetrahydrofolateBy similarity1
Binding sitei535dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi477 – 478dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi524 – 527dUMPBy similarity4
Nucleotide bindingi565 – 567dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-113510. E2F mediated regulation of DNA replication.
R-SPO-500753. Pyrimidine biosynthesis.
R-SPO-69205. G1/S-Specific Transcription.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
ORF Names:SPAC15E1.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC15E1.04.
PomBaseiSPAC15E1.04.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003108251 – 625Probable thymidylate synthaseAdd BLAST625

Proteomic databases

MaxQBiQ9UTI7.
PRIDEiQ9UTI7.

Interactioni

Protein-protein interaction databases

BioGridi279249. 7 interactors.
MINTiMINT-4710571.

Structurei

3D structure databases

ProteinModelPortaliQ9UTI7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi229 – 255Asp-richSequence analysisAdd BLAST27

Sequence similaritiesi

In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.Sequence analysis
In the C-terminal section; belongs to the thymidylate synthase family.Sequence analysis

Phylogenomic databases

InParanoidiQ9UTI7.
KOiK00560.
OrthoDBiEOG092C3QQ6.
PhylomeDBiQ9UTI7.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
3.40.50.1950. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR003382. Flavoprotein.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF02441. Flavoprotein. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF52507. SSF52507. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UTI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPLHARFA TRAVKNPMIL EKERQLTDSK YHILVAATGS VAAIKLTLIV
60 70 80 90 100
KSLLTYKGVD VQVVLTDPAR NFVEKEDLTA LGVNVYNNAD DWKNWDGLEC
110 120 130 140 150
PITHIELRRW AHLLLIAPLS ANTMAKMANG LCDNLLTSLI RAWAPLKPIL
160 170 180 190 200
LAPAMNTLMW TNPITQEHLS AISRIYKNSE FIMPIEKVLA CGDIGMGGMA
210 220 230 240 250
EWRNIVGRVA DKLQLEQKSV LPNAVKNIDG QDDDSSEQTA AFEEYDDDDD
260 270 280 290 300
DDVDDNEQSN SMIETSANAD ITPKASLLPS TTESSISKDH ETSQAPLGSE
310 320 330 340 350
SVDTQASENV TTKPEPPVPF TSSEYRNTEE EQYLNLIRYI LENGQSRPDR
360 370 380 390 400
TGTGTRSVFA PPQLRFSLRN NTLPLLTTKR VFLRGVLEEL LWFIHGDTNA
410 420 430 440 450
NHLSEKGIHI WDGNGSREFL DSRGLTDRKV GDLGPIYGFQ WRHFGAQYVD
460 470 480 490 500
CDTDYTNKGV DQLAQVISTL KLNPYDRRII LSAWNPLAIP EMALPPCHIF
510 520 530 540 550
CQFYVSEPCK PGGKPQLSSM MYQRSADMGL GVPFNIASYS LLTHMIAHMC
560 570 580 590 600
GYEAAEFVHV MGDCHIYNDH LEALQTQLER VPKAFPKLFF KRDAKDIGSI
610 620
DSFSVDDFAV EGYNPYGPIK MKMSV
Length:625
Mass (Da):69,893
Last modified:May 1, 2000 - v1
Checksum:iB3BC6034BF26F61E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB52423.1.
PIRiT37719.
RefSeqiNP_594305.1. NM_001019728.2.

Genome annotation databases

EnsemblFungiiSPAC15E1.04.1; SPAC15E1.04.1:pep; SPAC15E1.04.
GeneIDi2542801.
KEGGispo:SPAC15E1.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB52423.1.
PIRiT37719.
RefSeqiNP_594305.1. NM_001019728.2.

3D structure databases

ProteinModelPortaliQ9UTI7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279249. 7 interactors.
MINTiMINT-4710571.

Proteomic databases

MaxQBiQ9UTI7.
PRIDEiQ9UTI7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC15E1.04.1; SPAC15E1.04.1:pep; SPAC15E1.04.
GeneIDi2542801.
KEGGispo:SPAC15E1.04.

Organism-specific databases

EuPathDBiFungiDB:SPAC15E1.04.
PomBaseiSPAC15E1.04.

Phylogenomic databases

InParanoidiQ9UTI7.
KOiK00560.
OrthoDBiEOG092C3QQ6.
PhylomeDBiQ9UTI7.

Enzyme and pathway databases

UniPathwayiUPA00575.
ReactomeiR-SPO-113510. E2F mediated regulation of DNA replication.
R-SPO-500753. Pyrimidine biosynthesis.
R-SPO-69205. G1/S-Specific Transcription.

Miscellaneous databases

PROiQ9UTI7.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
3.40.50.1950. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR003382. Flavoprotein.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF02441. Flavoprotein. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF52507. SSF52507. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_SCHPO
AccessioniPrimary (citable) accession number: Q9UTI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.