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Protein

Probable thymidylate synthase

Gene

SPAC15E1.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for both nuclear and mitochondrial DNA synthesis.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei497 – 4971By similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: PomBase
  2. thymidylate synthase activity Source: PomBase

GO - Biological processi

  1. dTMP biosynthetic process Source: PomBase
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

ReactomeiREACT_306641. E2F mediated regulation of DNA replication.
REACT_327756. Pyrimidine biosynthesis.
REACT_343222. G1/S-Specific Transcription.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
ORF Names:SPAC15E1.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC15E1.04.
PomBaseiSPAC15E1.04.

Subcellular locationi

  1. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 625625Probable thymidylate synthasePRO_0000310825Add
BLAST

Proteomic databases

MaxQBiQ9UTI7.

Interactioni

Protein-protein interaction databases

BioGridi279249. 3 interactions.
MINTiMINT-4710571.
STRINGi4896.SPAC15E1.04-1.

Structurei

3D structure databases

ProteinModelPortaliQ9UTI7.
SMRiQ9UTI7. Positions 327-625.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi229 – 25527Asp-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.Sequence Analysis
In the C-terminal section; belongs to the thymidylate synthase family.Sequence Analysis

Phylogenomic databases

eggNOGiCOG0452.
InParanoidiQ9UTI7.
KOiK00560.
OrthoDBiEOG7X6M9G.
PhylomeDBiQ9UTI7.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.50.1950. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR003382. Flavoprotein.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF02441. Flavoprotein. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF52507. SSF52507. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9UTI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPLHARFA TRAVKNPMIL EKERQLTDSK YHILVAATGS VAAIKLTLIV
60 70 80 90 100
KSLLTYKGVD VQVVLTDPAR NFVEKEDLTA LGVNVYNNAD DWKNWDGLEC
110 120 130 140 150
PITHIELRRW AHLLLIAPLS ANTMAKMANG LCDNLLTSLI RAWAPLKPIL
160 170 180 190 200
LAPAMNTLMW TNPITQEHLS AISRIYKNSE FIMPIEKVLA CGDIGMGGMA
210 220 230 240 250
EWRNIVGRVA DKLQLEQKSV LPNAVKNIDG QDDDSSEQTA AFEEYDDDDD
260 270 280 290 300
DDVDDNEQSN SMIETSANAD ITPKASLLPS TTESSISKDH ETSQAPLGSE
310 320 330 340 350
SVDTQASENV TTKPEPPVPF TSSEYRNTEE EQYLNLIRYI LENGQSRPDR
360 370 380 390 400
TGTGTRSVFA PPQLRFSLRN NTLPLLTTKR VFLRGVLEEL LWFIHGDTNA
410 420 430 440 450
NHLSEKGIHI WDGNGSREFL DSRGLTDRKV GDLGPIYGFQ WRHFGAQYVD
460 470 480 490 500
CDTDYTNKGV DQLAQVISTL KLNPYDRRII LSAWNPLAIP EMALPPCHIF
510 520 530 540 550
CQFYVSEPCK PGGKPQLSSM MYQRSADMGL GVPFNIASYS LLTHMIAHMC
560 570 580 590 600
GYEAAEFVHV MGDCHIYNDH LEALQTQLER VPKAFPKLFF KRDAKDIGSI
610 620
DSFSVDDFAV EGYNPYGPIK MKMSV
Length:625
Mass (Da):69,893
Last modified:May 1, 2000 - v1
Checksum:iB3BC6034BF26F61E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB52423.1.
PIRiT37719.
RefSeqiNP_594305.1. NM_001019728.2.

Genome annotation databases

EnsemblFungiiSPAC15E1.04.1; SPAC15E1.04.1:pep; SPAC15E1.04.
GeneIDi2542801.
KEGGispo:SPAC15E1.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB52423.1.
PIRiT37719.
RefSeqiNP_594305.1. NM_001019728.2.

3D structure databases

ProteinModelPortaliQ9UTI7.
SMRiQ9UTI7. Positions 327-625.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279249. 3 interactions.
MINTiMINT-4710571.
STRINGi4896.SPAC15E1.04-1.

Proteomic databases

MaxQBiQ9UTI7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC15E1.04.1; SPAC15E1.04.1:pep; SPAC15E1.04.
GeneIDi2542801.
KEGGispo:SPAC15E1.04.

Organism-specific databases

EuPathDBiFungiDB:SPAC15E1.04.
PomBaseiSPAC15E1.04.

Phylogenomic databases

eggNOGiCOG0452.
InParanoidiQ9UTI7.
KOiK00560.
OrthoDBiEOG7X6M9G.
PhylomeDBiQ9UTI7.

Enzyme and pathway databases

UniPathwayiUPA00575.
ReactomeiREACT_306641. E2F mediated regulation of DNA replication.
REACT_327756. Pyrimidine biosynthesis.
REACT_343222. G1/S-Specific Transcription.

Miscellaneous databases

NextBioi20803844.
PROiQ9UTI7.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.50.1950. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR003382. Flavoprotein.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF02441. Flavoprotein. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF52507. SSF52507. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTYSY_SCHPO
AccessioniPrimary (citable) accession number: Q9UTI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: May 1, 2000
Last modified: April 29, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.