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Protein

Probable thymidylate synthase

Gene

SPAC15E1.04

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for both nuclear and mitochondrial DNA synthesis.By similarity

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.By similarity

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei350dUMPBy similarity1
Active sitei497NucleophileBy similarity1
Binding sitei5275,10-methylenetetrahydrofolateBy similarity1
Binding sitei535dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi477 – 478dUMP; shared with dimeric partnerBy similarity2
Nucleotide bindingi524 – 527dUMPBy similarity4
Nucleotide bindingi565 – 567dUMPBy similarity3

GO - Molecular functioni

  • FMN binding Source: PomBase
  • phosphatase inhibitor activity Source: PomBase
  • phosphopantothenoylcysteine decarboxylase activity Source: PomBase
  • thymidylate synthase activity Source: PomBase

GO - Biological processi

  • coenzyme A biosynthetic process Source: PomBase
  • dTMP biosynthetic process Source: PomBase
  • dTTP biosynthetic process Source: UniProtKB-UniPathway
  • regulation of signal transduction Source: PomBase

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-499943 Interconversion of nucleotide di- and triphosphates
UniPathwayiUPA00575

Protein family/group databases

MoonProtiQ9UTI7

Names & Taxonomyi

Protein namesi
Recommended name:
Probable thymidylate synthase (EC:2.1.1.45)
Short name:
TS
Short name:
TSase
Gene namesi
ORF Names:SPAC15E1.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC15E1.04
PomBaseiSPAC15E1.04

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003108251 – 625Probable thymidylate synthaseAdd BLAST625

Proteomic databases

MaxQBiQ9UTI7
PaxDbiQ9UTI7
PRIDEiQ9UTI7

PTM databases

iPTMnetiQ9UTI7

Interactioni

Protein-protein interaction databases

BioGridi279249, 7 interactors
STRINGi4896.SPAC15E1.04.1

Structurei

3D structure databases

ProteinModelPortaliQ9UTI7
SMRiQ9UTI7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi229 – 255Asp-richSequence analysisAdd BLAST27

Sequence similaritiesi

In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.Sequence analysis
In the C-terminal section; belongs to the thymidylate synthase family.Sequence analysis

Phylogenomic databases

InParanoidiQ9UTI7
KOiK00560
OrthoDBiEOG092C3QQ6
PhylomeDBiQ9UTI7

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
3.40.50.1950, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR036551 Flavin_trans-like
IPR003382 Flavoprotein
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF02441 Flavoprotein, 1 hit
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF52507 SSF52507, 1 hit
SSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UTI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQPLHARFA TRAVKNPMIL EKERQLTDSK YHILVAATGS VAAIKLTLIV
60 70 80 90 100
KSLLTYKGVD VQVVLTDPAR NFVEKEDLTA LGVNVYNNAD DWKNWDGLEC
110 120 130 140 150
PITHIELRRW AHLLLIAPLS ANTMAKMANG LCDNLLTSLI RAWAPLKPIL
160 170 180 190 200
LAPAMNTLMW TNPITQEHLS AISRIYKNSE FIMPIEKVLA CGDIGMGGMA
210 220 230 240 250
EWRNIVGRVA DKLQLEQKSV LPNAVKNIDG QDDDSSEQTA AFEEYDDDDD
260 270 280 290 300
DDVDDNEQSN SMIETSANAD ITPKASLLPS TTESSISKDH ETSQAPLGSE
310 320 330 340 350
SVDTQASENV TTKPEPPVPF TSSEYRNTEE EQYLNLIRYI LENGQSRPDR
360 370 380 390 400
TGTGTRSVFA PPQLRFSLRN NTLPLLTTKR VFLRGVLEEL LWFIHGDTNA
410 420 430 440 450
NHLSEKGIHI WDGNGSREFL DSRGLTDRKV GDLGPIYGFQ WRHFGAQYVD
460 470 480 490 500
CDTDYTNKGV DQLAQVISTL KLNPYDRRII LSAWNPLAIP EMALPPCHIF
510 520 530 540 550
CQFYVSEPCK PGGKPQLSSM MYQRSADMGL GVPFNIASYS LLTHMIAHMC
560 570 580 590 600
GYEAAEFVHV MGDCHIYNDH LEALQTQLER VPKAFPKLFF KRDAKDIGSI
610 620
DSFSVDDFAV EGYNPYGPIK MKMSV
Length:625
Mass (Da):69,893
Last modified:May 1, 2000 - v1
Checksum:iB3BC6034BF26F61E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB52423.1
PIRiT37719
RefSeqiNP_594305.1, NM_001019728.2

Genome annotation databases

EnsemblFungiiSPAC15E1.04.1; SPAC15E1.04.1:pep; SPAC15E1.04
GeneIDi2542801
KEGGispo:SPAC15E1.04

Similar proteinsi

Entry informationi

Entry nameiTYSY_SCHPO
AccessioniPrimary (citable) accession number: Q9UTI7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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