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Protein

Probable dihydrofolate synthetase

Gene

fol3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Glutamate-adding enzyme which catalyzes the binding of the first glutamyl side chain to dihydropteroate. Leads to the de nove synthesis of tetrahydrofolate. de novo (By similarity).By similarity

Catalytic activityi

ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate.

Pathwayi: tetrahydrofolylpolyglutamate biosynthesis

This protein is involved in the pathway tetrahydrofolylpolyglutamate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolylpolyglutamate biosynthesis and in Cofactor biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 367ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00850.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dihydrofolate synthetase (EC:6.3.2.12)
Short name:
DHFS
Gene namesi
Name:fol3
ORF Names:SPAC227.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC227.09.
PomBaseiSPAC227.09. fol3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Probable dihydrofolate synthetasePRO_0000339137Add
BLAST

Proteomic databases

MaxQBiQ9UTD0.
PRIDEiQ9UTD0.

Interactioni

Protein-protein interaction databases

MINTiMINT-4710146.

Structurei

3D structure databases

ProteinModelPortaliQ9UTD0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the folylpolyglutamate synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000019981.
InParanoidiQ9UTD0.
KOiK01930.
OMAiPIANIDR.
OrthoDBiEOG7NKKX2.
PhylomeDBiQ9UTD0.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR001645. Folylpolyglutamate_synth.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
PANTHERiPTHR11136. PTHR11136. 1 hit.
PfamiPF08245. Mur_ligase_M. 1 hit.
[Graphical view]
PIRSFiPIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UTD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIQLGLQRM LQLLKHLGNP QESFCAVQIA GTNGKGSICS YIYTSLLQAA
60 70 80 90 100
IKTGRYTSPH FLEPRDTISI NGQIASEEIF NTCWKQVIEV DRRFRTKATE
110 120 130 140 150
FELLTATAFQ CFHHSGVRVA VIETGMGGRL DATNVFEEPV LSIISRICLD
160 170 180 190 200
HQAFLGNTLE AIAKEKAGIF KKNVPCVVDG LNEVNVLNQL KLSAEETRAH
210 220 230 240 250
PFYLAKGKSG ENKNEWIINT PNWGTNTFST PLKGDYQGQN LACAVTALDI
260 270 280 290 300
LSSSFSIMLP HVQNGVKNTS WPGRLDIRSV PSLGDILFDG AHNKEAAIEL
310 320 330 340 350
AKFVNSQRRE HNKSVSWVVA FTNTKDVTGI MKILLRKGDT VIATNFSSVS
360 370 380 390 400
GMPWIKSMEP EVIKNSISSE SSVECYTADN LTISEILRLA KEKNSSVIVC
410
GSLYLLGDMY RYLKLDV
Length:417
Mass (Da):46,112
Last modified:May 1, 2000 - v1
Checksum:i1816F05D68766EB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB61458.1.
PIRiT50165.
RefSeqiNP_592963.1. NM_001018363.2.

Genome annotation databases

EnsemblFungiiSPAC227.09.1; SPAC227.09.1:pep; SPAC227.09.
GeneIDi2541481.
KEGGispo:SPAC227.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB61458.1.
PIRiT50165.
RefSeqiNP_592963.1. NM_001018363.2.

3D structure databases

ProteinModelPortaliQ9UTD0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4710146.

Proteomic databases

MaxQBiQ9UTD0.
PRIDEiQ9UTD0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC227.09.1; SPAC227.09.1:pep; SPAC227.09.
GeneIDi2541481.
KEGGispo:SPAC227.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC227.09.
PomBaseiSPAC227.09. fol3.

Phylogenomic databases

HOGENOMiHOG000019981.
InParanoidiQ9UTD0.
KOiK01930.
OMAiPIANIDR.
OrthoDBiEOG7NKKX2.
PhylomeDBiQ9UTD0.

Enzyme and pathway databases

UniPathwayiUPA00850.

Miscellaneous databases

NextBioi20802580.
PROiQ9UTD0.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR001645. Folylpolyglutamate_synth.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
PANTHERiPTHR11136. PTHR11136. 1 hit.
PfamiPF08245. Mur_ligase_M. 1 hit.
[Graphical view]
PIRSFiPIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiFOLD_SCHPO
AccessioniPrimary (citable) accession number: Q9UTD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 1, 2000
Last modified: November 11, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.