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Protein

tRNA wybutosine-synthesizing protein 3

Gene

tyw3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72 (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-(3-amino-3-carboxypropyl))-4-demethylwyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-(3-amino-3-carboxypropyl))wyosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 3 (EC:2.1.1.282)
Short name:
tRNA-yW-synthesizing protein 3
Alternative name(s):
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase
Gene namesi
Name:tyw3
ORF Names:SPAC25B8.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC25B8.15c.
PomBaseiSPAC25B8.15c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237tRNA wybutosine-synthesizing protein 3PRO_0000281848Add
BLAST

Proteomic databases

MaxQBiQ9UTA5.

Interactioni

Protein-protein interaction databases

BioGridi279180. 1 interaction.
MINTiMINT-4709972.

Family & Domainsi

Sequence similaritiesi

Belongs to the TYW3 family.Curated

Phylogenomic databases

HOGENOMiHOG000247848.
InParanoidiQ9UTA5.
KOiK15450.
OMAiVIMINET.
OrthoDBiEOG73V6WR.
PhylomeDBiQ9UTA5.

Family and domain databases

Gene3Di3.30.1960.10. 1 hit.
InterProiIPR003827. tRNA_yW-synthesising.
[Graphical view]
PfamiPF02676. TYW3. 1 hit.
[Graphical view]
SUPFAMiSSF111278. SSF111278. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9UTA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKGIDVSF DAQKKEILEG LKSSVPDASP KGHPDSPIFP LLDVINSHPD
60 70 80 90 100
WVTTSSCSGR ISVYVQGANS RKGGGYWLFV SHQAHEELPP VLEDEKVEYG
110 120 130 140 150
KVPSSPVEGN REIQYAFEPM ILHVQTRSLA NAQHLQRVAA SCGFRETGIQ
160 170 180 190 200
GSEQKFIVAI RTSLRMDIPI GCLTASEKLQ FYITREYMCF LFKRSVEYFT
210 220 230
ENGNRMARLK EQLERQVEKR MKPRRKLRNM DDYLVQS
Length:237
Mass (Da):26,997
Last modified:May 1, 2000 - v1
Checksum:i29A6CF0D0C720997
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB61781.1.
PIRiT50202.
RefSeqiNP_594475.1. NM_001019904.2.

Genome annotation databases

EnsemblFungiiSPAC25B8.15c.1; SPAC25B8.15c.1:pep; SPAC25B8.15c.
GeneIDi2542730.
KEGGispo:SPAC25B8.15c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB61781.1.
PIRiT50202.
RefSeqiNP_594475.1. NM_001019904.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279180. 1 interaction.
MINTiMINT-4709972.

Proteomic databases

MaxQBiQ9UTA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC25B8.15c.1; SPAC25B8.15c.1:pep; SPAC25B8.15c.
GeneIDi2542730.
KEGGispo:SPAC25B8.15c.

Organism-specific databases

EuPathDBiFungiDB:SPAC25B8.15c.
PomBaseiSPAC25B8.15c.

Phylogenomic databases

HOGENOMiHOG000247848.
InParanoidiQ9UTA5.
KOiK15450.
OMAiVIMINET.
OrthoDBiEOG73V6WR.
PhylomeDBiQ9UTA5.

Enzyme and pathway databases

UniPathwayiUPA00375.

Miscellaneous databases

NextBioi20803775.
PROiQ9UTA5.

Family and domain databases

Gene3Di3.30.1960.10. 1 hit.
InterProiIPR003827. tRNA_yW-synthesising.
[Graphical view]
PfamiPF02676. TYW3. 1 hit.
[Graphical view]
SUPFAMiSSF111278. SSF111278. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiTYW3_SCHPO
AccessioniPrimary (citable) accession number: Q9UTA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 1, 2000
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.