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Protein

tRNA wybutosine-synthesizing protein 3

Gene

tyw3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72 (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-(3-amino-3-carboxypropyl))-4-demethylwyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-(3-amino-3-carboxypropyl))wyosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 3 (EC:2.1.1.282)
Short name:
tRNA-yW-synthesizing protein 3
Alternative name(s):
tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase
Gene namesi
Name:tyw3
ORF Names:SPAC25B8.15c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC25B8.15c
PomBaseiSPAC25B8.15c tyw3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002818481 – 237tRNA wybutosine-synthesizing protein 3Add BLAST237

Proteomic databases

MaxQBiQ9UTA5
PaxDbiQ9UTA5
PRIDEiQ9UTA5

PTM databases

iPTMnetiQ9UTA5

Interactioni

Protein-protein interaction databases

BioGridi279180, 1 interactor
STRINGi4896.SPAC25B8.15c.1

Structurei

3D structure databases

SMRiQ9UTA5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TYW3 family.Curated

Phylogenomic databases

HOGENOMiHOG000247848
InParanoidiQ9UTA5
KOiK15450
OMAiLHVKCRD
OrthoDBiEOG092C5EC9
PhylomeDBiQ9UTA5

Family and domain databases

Gene3Di3.30.1960.10, 1 hit
InterProiView protein in InterPro
IPR003827 tRNA_yW-synthesising
IPR036602 tRNA_yW-synthesising-like_sf
PfamiView protein in Pfam
PF02676 TYW3, 1 hit
SUPFAMiSSF111278 SSF111278, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UTA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKGIDVSF DAQKKEILEG LKSSVPDASP KGHPDSPIFP LLDVINSHPD
60 70 80 90 100
WVTTSSCSGR ISVYVQGANS RKGGGYWLFV SHQAHEELPP VLEDEKVEYG
110 120 130 140 150
KVPSSPVEGN REIQYAFEPM ILHVQTRSLA NAQHLQRVAA SCGFRETGIQ
160 170 180 190 200
GSEQKFIVAI RTSLRMDIPI GCLTASEKLQ FYITREYMCF LFKRSVEYFT
210 220 230
ENGNRMARLK EQLERQVEKR MKPRRKLRNM DDYLVQS
Length:237
Mass (Da):26,997
Last modified:May 1, 2000 - v1
Checksum:i29A6CF0D0C720997
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB61781.1
PIRiT50202
RefSeqiNP_594475.1, NM_001019904.2

Genome annotation databases

EnsemblFungiiSPAC25B8.15c.1; SPAC25B8.15c.1:pep; SPAC25B8.15c
GeneIDi2542730
KEGGispo:SPAC25B8.15c

Similar proteinsi

Entry informationi

Entry nameiTYW3_SCHPO
AccessioniPrimary (citable) accession number: Q9UTA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health